2020
Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores
Mudumbi KC, Czapiewski R, Ruba A, Junod SL, Li Y, Luo W, Ngo C, Ospina V, Schirmer EC, Yang W. Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores. Nature Communications 2020, 11: 2184. PMID: 32366843, PMCID: PMC7198523, DOI: 10.1038/s41467-020-16033-x.Peer-Reviewed Original ResearchConceptsMembrane proteinsInner nuclear membrane proteinCentral channelEukaryotic transmembrane proteinsNuclear membrane proteinsNuclear localization signalNuclear pore complexCertain membrane proteinsCentral outstanding questionsSingle-molecule microscopyProtein importProtein translocationLocalization signalPore complexNuclear transportBlock translocationTransmembrane proteinNuclear poresSuch proteinsProtein targetsNuclear membraneProteinTranslocationOutstanding questionsChannel transport
2019
Casting a Wider Net: Differentiating between Inner Nuclear Envelope and Outer Nuclear Envelope Transmembrane Proteins
Tingey M, Mudumbi KC, Schirmer EC, Yang W. Casting a Wider Net: Differentiating between Inner Nuclear Envelope and Outer Nuclear Envelope Transmembrane Proteins. International Journal Of Molecular Sciences 2019, 20: 5248. PMID: 31652739, PMCID: PMC6862087, DOI: 10.3390/ijms20215248.Peer-Reviewed Original ResearchConceptsNuclear envelope transmembrane proteinsEnvelope transmembrane proteinsInner nuclear membraneOuter nuclear membraneNuclear envelopeTransmembrane proteinDouble membraneNuclear membraneInner nuclear envelopeGenome architectureNuclear organizationNuclear positioningEukaryotic cellsCellular functionsDNA replicationEndoplasmic reticulumCorrect localizationProteinVital functionsMembraneNuclear structureImportant roleSplicingEpigeneticsNuclear stability
2017
Determination of Membrane Protein Distribution on the Nuclear Envelope by Single‐Point Single‐Molecule FRAP
Mudumbi K, Yang W. Determination of Membrane Protein Distribution on the Nuclear Envelope by Single‐Point Single‐Molecule FRAP. Current Protocols In Cell Biology 2017, 76: 21.11.1-21.11.13. PMID: 28862339, PMCID: PMC5879780, DOI: 10.1002/cpcb.27.Peer-Reviewed Original ResearchConceptsNuclear envelope transmembrane proteinsInner nuclear membraneOuter nuclear membraneNuclear membraneEnvelope transmembrane proteinsEukaryotic cellsTransmembrane proteinEndoplasmic reticulumHuman diseasesFluorescence recoveryTranslocation rateTranslocation dynamicsMembraneAbnormal distributionProteinReticulumMicroscopy techniquesCellsVivoSpatial distribution
2016
Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
Mudumbi K, Schirmer E, Yang W. Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution. Nature Communications 2016, 7: 12562. PMID: 27558844, PMCID: PMC5007294, DOI: 10.1038/ncomms12562.Peer-Reviewed Original ResearchConceptsNuclear envelope transmembrane proteinsInner nuclear membraneOuter nuclear membraneNuclear membraneEnvelope transmembrane proteinsMembrane protein distributionTranslocation rateTransmembrane proteinEndoplasmic reticulumProtein distributionHuman diseasesMembraneProteinReticulumMicroscopy techniquesDetermination of distributionVivoProbing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP
Mudumbi K, Yang W. Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP. Methods In Molecular Biology 2016, 1411: 113-122. PMID: 27147037, PMCID: PMC10099394, DOI: 10.1007/978-1-4939-3530-7_6.Peer-Reviewed Original ResearchConceptsNuclear envelope transmembrane proteinsNuclear membrane proteinsEnvelope transmembrane proteinsInner nuclear membraneNuclear envelope membranesWeeks-long processEnvelope membraneMembrane proteinsTransmembrane proteinNuclear envelopeNuclear membraneProtein distributionFluorescence recoveryStaining of cellsProteinMembraneElectron microscopy