2024
Multiple roles for AU-rich RNA binding proteins in the development of haematologic malignancies and their resistance to chemotherapy
Podszywalow-Bartnicka P, Neugebauer K. Multiple roles for AU-rich RNA binding proteins in the development of haematologic malignancies and their resistance to chemotherapy. RNA Biology 2024, 21: 1-17. PMID: 38798162, PMCID: PMC11135835, DOI: 10.1080/15476286.2024.2346688.Peer-Reviewed Original ResearchConceptsARE-binding proteinsRNA-binding proteinsAU-rich elementsStress granulesBinding proteinTranslational regulation of mRNAsImpact alternative splicingCytoplasmic stress granulesProtein-RNA bindingAdaptation to microenvironmentProtein-RNA networksBinding to AU-rich elementsCancer cell proteomePost-transcriptional regulationAU-rich RNA-binding proteinsRegulation of mRNAsChemotherapy resistanceGene expression levelsSequence motifsProtein-RNAMRNA structureMature mRNATranslational regulationAlternative splicingCell proteome
2022
Transcriptome-wide mapping reveals a diverse dihydrouridine landscape including mRNA
Draycott AS, Schaening-Burgos C, Rojas-Duran MF, Wilson L, Schärfen L, Neugebauer KM, Nachtergaele S, Gilbert WV. Transcriptome-wide mapping reveals a diverse dihydrouridine landscape including mRNA. PLOS Biology 2022, 20: e3001622. PMID: 35609439, PMCID: PMC9129914, DOI: 10.1371/journal.pbio.3001622.Peer-Reviewed Original ResearchConceptsTranscriptome-wide mappingSmall nucleolar RNAsFunctional RNA structuresSingle-nucleotide resolutionStem-loop regionEukaryotic ribosomesNucleolar RNAsPre-mRNARNA structureRNA targetsDihydrouridine synthaseHuman diseasesMRNARNANovel classFunctional componentsSplicingTRNARibosomesYeastDependent changesLandscapeOrganismsDihydrouridineSequencing
2019
Uncoupling of nucleo-cytoplasmic RNA export and localization during stress
Hochberg-Laufer H, Schwed-Gross A, Neugebauer KM, Shav-Tal Y. Uncoupling of nucleo-cytoplasmic RNA export and localization during stress. Nucleic Acids Research 2019, 47: 4778-4797. PMID: 30864659, PMCID: PMC6511838, DOI: 10.1093/nar/gkz168.Peer-Reviewed Original ResearchConceptsRNA-binding proteinStress granulesNuclear specklesMRNA exportFormation of SGsCytoplasmic stress granulesSub-cellular compartmentsGene expression pathwaysMRNA export adaptorsComplex recruitmentEukaryotic cellsRNA exportRNA metabolismExport adaptorGranule assemblyNuclear proteinsExpression pathwaysRNAStress inductionMRNACytoplasmProteinPotent inhibitorCellsNucleoporins
2017
Cellular differentiation state modulates the mRNA export activity of SR proteins
Botti V, McNicoll F, Steiner MC, Richter FM, Solovyeva A, Wegener M, Schwich OD, Poser I, Zarnack K, Wittig I, Neugebauer KM, Müller-McNicoll M. Cellular differentiation state modulates the mRNA export activity of SR proteins. Journal Of Cell Biology 2017, 216: 1993-2009. PMID: 28592444, PMCID: PMC5496613, DOI: 10.1083/jcb.201610051.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsArginineCell DifferentiationCell NucleusDNA-Binding ProteinsHeLa CellsHumansImmunoprecipitationMethylationMiceNeurogenesisPhenotypePhosphorylationPluripotent Stem CellsProtein BindingProtein Processing, Post-TranslationalRepressor ProteinsRNA InterferenceRNA, MessengerRNA-Binding ProteinsSerine-Arginine Splicing FactorsTandem Mass SpectrometryTranscription FactorsTransfectionConceptsMRNA export activitySR proteinsP19 cellsMRNA exportSR protein family membersProtein-RNA interactionsMurine P19 cellsCellular differentiation stateProtein family membersLower phosphorylation levelsArginine methylationPluripotency factorsCytoplasmic mRNA levelsMRNA processingPosttranslational modificationsCellular dynamicsDifferentiated cellsNeural differentiationSRSF5Differentiation statePhosphorylation levelsHeLa cellsProteinExport activityMRNA levelsPurification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
Heyn P, Neugebauer KM. Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos. Methods In Molecular Biology 2017, 1605: 121-131. PMID: 28456961, DOI: 10.1007/978-1-4939-6988-3_8.Peer-Reviewed Original Research
2014
The Earliest Transcribed Zygotic Genes Are Short, Newly Evolved, and Different across Species
Heyn P, Kircher M, Dahl A, Kelso J, Tomancak P, Kalinka AT, Neugebauer KM. The Earliest Transcribed Zygotic Genes Are Short, Newly Evolved, and Different across Species. Cell Reports 2014, 6: 285-292. PMID: 24440719, DOI: 10.1016/j.celrep.2013.12.030.Peer-Reviewed Original ResearchConceptsZygotic genome activationZygotic genesEarly zygotic transcriptsOne-cell stageSignificant transcriptional activityRapid cell divisionComparison of fishGenome activationMitochondrial genomePurification of RNAZygotic controlEvolutionary plasticityMulticellular organismsNuclear genesZygotic transcriptsEarly embryogenesisShort genesDanio rerioAdaptive potentialCell divisionTranscriptional activityMetabolic labelingCell cycleEarly genesGenes
2011
The In Vivo Kinetics of RNA Polymerase II Elongation during Co-Transcriptional Splicing
Brody Y, Neufeld N, Bieberstein N, Causse SZ, Böhnlein EM, Neugebauer KM, Darzacq X, Shav-Tal Y. The In Vivo Kinetics of RNA Polymerase II Elongation during Co-Transcriptional Splicing. PLOS Biology 2011, 9: e1000573. PMID: 21264352, PMCID: PMC3019111, DOI: 10.1371/journal.pbio.1000573.Peer-Reviewed Original ResearchMeSH KeywordsBeta-GlobinsFluorescence Recovery After PhotobleachingGreen Fluorescent ProteinsHumansIntronsInverted Repeat SequencesLac RepressorsRecombinant Fusion ProteinsRibonucleoproteins, Small NuclearRNA Polymerase IIRNA PrecursorsRNA SplicingRNA, MessengerSpliceosomesTranscription, GeneticTumor Cells, CulturedConceptsNumber of intronsU1 snRNPPol II elongation ratesRNA polymerase II elongationRNA polymerase II enzymeSplicing-independent roleTranscription elongation kineticsIntron-containing genesCo-transcriptional splicingPolymerase II elongationCompletion of splicingTranscribed pre-mRNARNA processing eventsInducible gene constructsPol II elongationElongation ratePolymerase elongation ratesNascent RNATranscriptional elongationIntronless genesSpliceosome componentsTranscription terminationSplicing machineryTranscription sitesGene end
2010
The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells
Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Staněk D. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. Journal Of Cell Biology 2010, 191: 75-86. PMID: 20921136, PMCID: PMC2953428, DOI: 10.1083/jcb.201004030.Peer-Reviewed Original ResearchConceptsSmall nuclear RNP particlesPrecursor messenger RNA splicingMessenger RNA splicingBinding of U1Live-cell imagingRate of splicingNuclear RNP particlesLarge ribonucleoproteinSnRNP componentsRNA splicingSpliceosome assemblyAdditional proteinsRNP particlesHuman cellsSplicingLiving cellsCell nucleiDifferential interactionsEndogenous levelsSpliceosomeMRNARibonucleoproteinCell imagingCore componentCellsBinding properties and dynamic localization of an alternative isoform of the cap-binding complex subunit CBP20
Pabis M, Neufeld N, Shav-Tal Y, Neugebauer KM. Binding properties and dynamic localization of an alternative isoform of the cap-binding complex subunit CBP20. Nucleus 2010, 1: 412-421. PMID: 21326824, PMCID: PMC3037537, DOI: 10.4161/nucl.1.5.12839.Peer-Reviewed Original ResearchConceptsCap-binding complexRNA recognition motifSmall nuclear RNAMessenger RNAsRNA polymerase II transcriptsNuclear cap-binding complexActive transcription sitesPolymerase II transcriptsM7G capAlternative splice variantsGuanosine capRNA processingCBP80 subunitHuman cell linesCBP20Transcription sitesG capAlternative isoformsNuclear RNATranscription inhibitionRecognition motifMammalian speciesCBP80High-affinity bindingFrame deletionGlobal analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells
Änkö M, Morales L, Henry I, Beyer A, Neugebauer KM. Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells. Nature Structural & Molecular Biology 2010, 17: 962-970. PMID: 20639886, DOI: 10.1038/nsmb.1862.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell DifferentiationCell Line, TumorChromosomes, Artificial, BacterialGene Expression Regulation, NeoplasticGene Knockdown TechniquesGreen Fluorescent ProteinsImmunoprecipitationMiceNeuronsOrgan SpecificityProtein BindingRibonucleoproteinsRNA, MessengerRNA-Binding ProteinsSerine-Arginine Splicing Factors
1997
Transcription units as RNA processing units
Neugebauer K, Roth M. Transcription units as RNA processing units. Genes & Development 1997, 11: 3279-3285. PMID: 9407022, DOI: 10.1101/gad.11.24.3279.Peer-Reviewed Original Research