Discovery Highlights
Drosophila hemibrain generated by two Enhanced FIB-SEM systems through 13 hot-knife tabs
A connectome of the adult Drosophila central brain
Xu CS, Januszewski M, Lu Z, Takemura S, Hayworth K, et al. bioRxiv 2020.01.21.911859; DOI: 10.1101/2020.0
The largest and most detailed brain connectome to date—Drosophila “hemi-brain” connectome
A connectome and analysis of the adult Drosophila central brain
Scheffer LK†*, Xu CS†, Januszewski M†, Lu Z†, Takemura S†, Hayworth KJ†, Huang GB†, Shinomiya K†, et al. eLife 2020; 9: e57443. DOI: 10.7554/eLife.57443.
3D whole cell imaging at the finest isotropic resolution
An open-access volume electron microscopy atlas of whole cells and tissues.
Xu CS*, Pang S, Shtengel G, Müller A, et al. Nature 2021; 599 147-151. DOI: 10.1038/s41586-021-03992-4.
Segmentation pipeline: from FIB-SEM raw data to training data to predictions
Whole-cell organelle segmentation in volume electron microscopy
Heinrich L, Bennett D, Ackerman D, et al. Nature 2021; 599 141-146. DOI: 10.1038/s41586-021-03977-3.
The first 3D reconstruction of a hepatic endoplasmic reticulum and mitochondria in liver from lean to obese mice
Regulation of liver subcelluar architecture controls metabolic homeostasis.
Parlakgül G, Arruda AP, Pang S, et al. Nature 2022; 603, 736-742. DOI: 10.1038/s41586-022-04488-5
The first comprehensive 3D representation of microtube networks and their interaction with other organelles in a mouse pancreatic islet beta cell
3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cell.
Müller A*, Schmidt D, Xu CS, Pang S, et al. JCB 2021; 220(2): e202010039. DOI: 10.1083/jcb.202010039.
The first comprehensive 3D visualization of a T cell attaching a cancer cell
ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack
Ritter AT*, Shtengel G, Xu CS, et al. Science 2022; Apr 21, 376(6591). DOI: 10.1126/science.abl3855
Correlative cryogenic 3D super-resolution and 3D FIB-SEM of whole vitreously frozen cells
Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells.
Hoffman DP†, Shtengel G†, Xu CS, et al. Science 2020; Jan 17, 367(6475). DOI: 10.1126/science.aaz5357.
Organization of the ER in dendrites
Contacts between the endoplasmic reticulum and other membranes in neurons.
Wu Y, Whiteus C, Xu CS, et al. PNAS 2017; 114(24), E4859–E4867. DOI: 10.1073/pnas.1701078114.
Drosophila optic lobe FIB-SEM 3-months run
Enhanced FIB-SEM systems for large-volume 3D imaging
Xu CS*, Hayworth KJ, Lu Z, et al. eLife 2017; 6: e25916. DOI: 10.7554/eLife.25916.
Myelinated axons in mouse hippocampus
Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution
Gao R†, Asano SM†, Upadhyayula S†, et al. Science 2019; Jan 18; 363(6424). DOI: 10.1126/science.aau8302.
Convoluted ER matrix
Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER
Nixon-Abell J†, Obara CJ†, Weigel AV†, et al. Science 2016; 354(6311):433-446. DOI: 10.1126/science.aaf3928