2024
Heterogeneity of hepatocyte dynamics restores liver architecture after chemical, physical or viral damage
Ruz-Maldonado I, Gonzalez J, Zhang H, Sun J, Bort A, Kabir I, Kibbey R, Suárez Y, Greif D, Fernández-Hernando C. Heterogeneity of hepatocyte dynamics restores liver architecture after chemical, physical or viral damage. Nature Communications 2024, 15: 1247. PMID: 38341404, PMCID: PMC10858916, DOI: 10.1038/s41467-024-45439-0.Peer-Reviewed Original Research
2023
The age of bone marrow dictates the clonality of smooth muscle-derived cells in atherosclerotic plaques
Kabir I, Zhang X, Dave J, Chakraborty R, Qu R, Chandran R, Ntokou A, Gallardo-Vara E, Aryal B, Rotllan N, Garcia-Milian R, Hwa J, Kluger Y, Martin K, Fernández-Hernando C, Greif D. The age of bone marrow dictates the clonality of smooth muscle-derived cells in atherosclerotic plaques. Nature Aging 2023, 3: 64-81. PMID: 36743663, PMCID: PMC9894379, DOI: 10.1038/s43587-022-00342-5.Peer-Reviewed Original ResearchConceptsAtherosclerotic plaquesBone marrowSmooth muscle-derived cellsSMC progenitorsAtherosclerotic plaque cellsSmooth muscle cell progenitorsPredominant risk factorCause of deathNovel therapeutic strategiesTNF receptor 1Muscle-derived cellsAged bone marrowAged BMEffect of agePlaque burdenAged miceRisk factorsTumor necrosisTherapeutic strategiesPlaque cellsMyeloid cellsReceptor 1Integrin β3Cell progenitorsAtherosclerosis
2021
Distinct roles of KLF4 in mesenchymal cell subtypes during lung fibrogenesis
Chandran RR, Xie Y, Gallardo-Vara E, Adams T, Garcia-Milian R, Kabir I, Sheikh AQ, Kaminski N, Martin KA, Herzog EL, Greif DM. Distinct roles of KLF4 in mesenchymal cell subtypes during lung fibrogenesis. Nature Communications 2021, 12: 7179. PMID: 34893592, PMCID: PMC8664937, DOI: 10.1038/s41467-021-27499-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationDisease Models, AnimalDown-RegulationExtracellular MatrixFemaleFibroblastsFibrosisHumansKruppel-Like Factor 4LungLung InjuryMaleMesenchymal Stem CellsMiceMice, Inbred C57BLMyofibroblastsReceptor, Platelet-Derived Growth Factor betaRespiratory Tract DiseasesSignal TransductionTransforming Growth Factor betaConceptsMesenchymal cell typesPlatelet-derived growth factor receptorSmooth muscle actinLung fibrosisKruppel-like factor 4Forkhead box M1Growth factor receptorCell transitionCell typesExtracellular matrixDistinct rolesKLF4Box M1C chemokine ligandMesenchymal cell subtypesFactor receptorPro-fibrotic effectsFactor 4PDGFRMesenchymeCellsMacrophage accumulationKLF4 levelsChemokine ligandLung fibrogenesis
2018
2-Hydroxy-oleic acid does not activate sphingomyelin synthase activity
Lou B, Liu Q, Hou J, Kabir I, Liu P, Ding T, Dong J, Mo M, Ye D, Chen Y, Bui HH, Roth K, Cao Y, Jiang XC. 2-Hydroxy-oleic acid does not activate sphingomyelin synthase activity. Journal Of Biological Chemistry 2018, 293: 18328-18336. PMID: 30305392, PMCID: PMC6254338, DOI: 10.1074/jbc.ra118.005904.Peer-Reviewed Original ResearchConceptsSMS activationAnticancer effectsCancer cell cycle arrestU118 cellsDose-dependent fashionSM activityPlasma phosphatidylcholinePredictive biomarkersTumor responseC57BL/6J miceCell cycle arrestTotal cellular phosphatidylcholinePotent anticancer drugLysophosphatidylcholine acyltransferase activitySphingomyelin levelsCancer cellsPhosphatidylcholine metabolismTissue homogenatesSphingomyelin accumulationCycle arrestSphingomyelin synthase activityMouse tissue homogenatesAnticancer propertiesCancer suppressionAcyltransferase activityIntegrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells
Misra A, Feng Z, Chandran RR, Kabir I, Rotllan N, Aryal B, Sheikh AQ, Ding L, Qin L, Fernández-Hernando C, Tellides G, Greif DM. Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells. Nature Communications 2018, 9: 2073. PMID: 29802249, PMCID: PMC5970166, DOI: 10.1038/s41467-018-04447-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaAtherosclerosisBone Marrow TransplantationCell MovementCell ProliferationCell TransdifferentiationCells, CulturedCholesterolDisease Models, AnimalFemaleHumansIntegrin beta3MacrophagesMaleMiceMice, Inbred C57BLMice, Knockout, ApoEMuscle, Smooth, VascularMyocytes, Smooth MusclePlaque, AtheroscleroticConceptsSmooth muscle cellsPre-existing smooth muscle cellsAtherosclerotic plaquesPlaque cellsToll-like receptor 4 expressionSmooth muscle-derived cellsBone marrow-derived cellsSingle smooth muscle cellsAtherosclerotic plaque cellsReceptor 4 expressionMarrow-derived cellsBone marrow resultsMuscle-derived cellsIntegrin β3 levelsMacrophage-like phenotypeCD36 levelsMarrow resultsSMC proliferationPlaque coresSMC progenitorsMuscle cellsIntegrin β3AtherogenesisPlaquesIntegrin beta3Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function
Li Z, Kabir I, Tietelman G, Huan C, Fan J, Worgall T, Jiang XC. Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function. Cell Death & Disease 2018, 9: 173. PMID: 29415989, PMCID: PMC5833386, DOI: 10.1038/s41419-017-0214-1.Peer-Reviewed Original ResearchConceptsTamoxifen treatmentBarrier functionInflammatory bowel diseaseGoblet cell numbersCell survivalConcurrent diarrheaSPTLC2 expressionIntestinal cell survivalRectal bleedingBowel diseaseRate-limiting enzymeBody weightColon specimensGoblet cellsSerine palmitoyltransferaseSmall intestineDay 6MiceEarly lifeIntestineReduced lethalitySPT activityCell numberSPTLC2Survival
2016
Liver serine palmitoyltransferase activity deficiency in early life impairs adherens junctions and promotes tumorigenesis
Li Z, Kabir I, Jiang H, Zhou H, Libien J, Zeng J, Stanek A, Ou P, Li KR, Zhang S, Bui HH, Kuo M, Park T, Kim B, Worgall TS, Huan C, Jiang X. Liver serine palmitoyltransferase activity deficiency in early life impairs adherens junctions and promotes tumorigenesis. Hepatology 2016, 64: 2089-2102. PMID: 27642075, PMCID: PMC5115983, DOI: 10.1002/hep.28845.Peer-Reviewed Original ResearchConceptsAdherens junctionsSerine palmitoyltransferaseHepatocyte polaritySubunit 2 geneCanonical Wnt pathwaySphingolipid biosynthesisPlasma membraneKey enzymeMajor proteinsWnt pathwayRecombination approachHepatocyte plasma membraneCellular distributionΒ-cateninTumorigenesisCadherin phosphorylationSphingomyelin levelsActivity deficiencyCentral componentPalmitoyltransferaseMembraneCadherinBiosynthesisLiver regenerationGenesSmall Intestine but Not Liver Lysophosphatidylcholine Acyltransferase 3 (Lpcat3) Deficiency Has a Dominant Effect on Plasma Lipid Metabolism*
Kabir I, Li Z, Bui HH, Kuo MS, Gao G, Jiang XC. Small Intestine but Not Liver Lysophosphatidylcholine Acyltransferase 3 (Lpcat3) Deficiency Has a Dominant Effect on Plasma Lipid Metabolism*. Journal Of Biological Chemistry 2016, 291: 7651-7660. PMID: 26828064, PMCID: PMC4817191, DOI: 10.1074/jbc.m115.697011.Peer-Reviewed Original ResearchMeSH Keywords1-Acylglycerophosphocholine O-AcyltransferaseAnimalsATP Binding Cassette Transporter 1ATP Binding Cassette Transporter, Subfamily G, Member 8ATP-Binding Cassette TransportersCD36 AntigensCell MembraneEnterocytesIntestine, SmallLipid MetabolismLipoproteinsLiverMembrane Transport ProteinsMiceMice, KnockoutOrgan SpecificityConceptsLpcat3 deficiencySmall intestineLipid levelsSignificant lipid accumulationLipid metabolismLiver deficiencyLipid accumulationPlasma lipid levelsPlasma lipid metabolismCassette transporter 1Plasma triglyceride levelsNiemann-Pick C1Lysophosphatidylcholine acyltransferase 3ABCG8 levelsCholesterol secretionTriglyceride levelsPlasma triglyceridesBody weightLipid absorptionPhospholipid levelsDay 9Transporter 1IntestineLiverEnterocyte plasma membrane
2015
All members in the sphingomyelin synthase gene family have ceramide phosphoethanolamine synthase activity[S]
Ding T, Kabir I, Li Y, Lou C, Yazdanyar A, Xu J, Dong J, Zhou H, Park T, Boutjdir M, Li Z, Jiang XC. All members in the sphingomyelin synthase gene family have ceramide phosphoethanolamine synthase activity[S]. Journal Of Lipid Research 2015, 56: 537-545. PMID: 25605874, PMCID: PMC4340302, DOI: 10.1194/jlr.m054627.Peer-Reviewed Original ResearchConceptsCeramide phosphoethanolamineCPE levelsSynthase activityObvious phenotypic alterationsSynthase gene familySf9 insect cellsQuantitative MS analysisGene familyCeramide homeostasisInsect cellsCritical regulatorNBD-ceramideSMS2 activityPhenotypic alterationsSMS1SMS1 deficiencySphingolipid levelsSMS2Ceramide levelsCell functionKO miceCell studiesMS analysisFamily membersCells