2000
Genetically and epidemiologically related “non‐syncytium‐inducing” isolates of HIV‐1 display heterogeneous growth patterns in macrophages
Jesus M, Anders C, Miller G, Sleasman J, Goodenow M, Andiman W. Genetically and epidemiologically related “non‐syncytium‐inducing” isolates of HIV‐1 display heterogeneous growth patterns in macrophages. Journal Of Medical Virology 2000, 61: 171-180. PMID: 10797371, DOI: 10.1002/(sici)1096-9071(200006)61:2<171::aid-jmv1>3.0.co;2-x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmino Acid SequenceAntigens, ViralCD4 AntigensCell LineCells, CulturedChild, PreschoolFemaleGiant CellsHIV Core Protein p24HIV InfectionsHIV-1HumansInfantLeukocytes, MononuclearMacrophagesMolecular Sequence DataPhenotypeProspective StudiesProtein Structure, TertiaryReceptors, ChemokineTransfectionTropismU937 CellsViral ProteinsVirus ReplicationConceptsNSI virusesDisease stageSurrogate markerPediatric human immunodeficiency virus type 1 (HIV-1) infectionHuman immunodeficiency virus type 1 (HIV-1) infectionVirus type 1 infectionCell linesCo-receptor preferenceM-tropic virusesType 1 infectionT-cell tropicDual tropicInducing (NSI) virusesNSI isolatesAsymptomatic childrenMacrophage-tropicClinical progressionSymptomatic childrenV3 loopNSI phenotypeHIV-1Lymphotropic virusCategory A1Heterogeneous growth patternsGenetic features
1999
Amino Acid Substitutions Reveal Distinct Functions of Serine 186 of the ZEBRA Protein in Activation of Early Lytic Cycle Genes and Synergy with the Epstein-Barr Virus R Transactivator
Francis A, Ragoczy T, Gradoville L, Heston L, El-Guindy A, Endo Y, Miller G. Amino Acid Substitutions Reveal Distinct Functions of Serine 186 of the ZEBRA Protein in Activation of Early Lytic Cycle Genes and Synergy with the Epstein-Barr Virus R Transactivator. Journal Of Virology 1999, 73: 4543-4551. PMID: 10233912, PMCID: PMC112494, DOI: 10.1128/jvi.73.6.4543-4551.1999.Peer-Reviewed Original ResearchConceptsLytic cycle genesLatent virusZEBRA proteinOverexpression of RtaEpstein-Barr virusLatent EBV genomeBRLF1 expressionEarly lytic cycle genesAmino acid substitutionsEBV genomeBRLF1BRLF1 promoterCycle genesAcid substitutionsVirusEpstein-Barr virus R transactivatorSerine 186Distinct phenotypesLytic life cycleR transactivatorActivationDifferent amino acid substitutionsResponse elementS186Expression
1998
High Prevalence of Antibodies to Human Herpesvirus 8 in Relatives of Patients with Classic Kaposi's Sarcoma from Sardinia
Angeloni A, Heston L, Uccini S, Sirianni M, Cottoni F, Masala M, Cerimele D, Lin S, Sun R, Rigsby M, Faggioni A, Miller G. High Prevalence of Antibodies to Human Herpesvirus 8 in Relatives of Patients with Classic Kaposi's Sarcoma from Sardinia. The Journal Of Infectious Diseases 1998, 177: 1715-1718. PMID: 9607855, DOI: 10.1086/517429.Peer-Reviewed Original ResearchConceptsSmall viral capsid antigenPrevalence of antibodiesClassic Kaposi's sarcomaHigh incidence areaKaposi's sarcomaActive HHV-8 infectionHuman herpesvirus type 8HHV-8 infectionViral capsid antigenRelatives of patientsHuman herpesvirus 8Familial risk factorsCapsid antigenRisk factorsHigh prevalenceKS patientsHerpesvirus 8SarcomaIncidence areaPopulation agesPatientsVertical transmissionPrevalenceAntibodiesType 8SEROLOGIC ASSOCIATION OF HUMAN HERPESVIRUS EIGHT WITH POSTTRANSPLANT KAPOSI'S SARCOMA IN SAUDI ARABIA1
Wajeh Q, Othman A, Khalid A, Lin3 4, Ren S, Lee H, Ross5 6, Rigsby7 8, Miller3 5. SEROLOGIC ASSOCIATION OF HUMAN HERPESVIRUS EIGHT WITH POSTTRANSPLANT KAPOSI'S SARCOMA IN SAUDI ARABIA1. Transplantation 1998, 65: 583-585. PMID: 9500639, DOI: 10.1097/00007890-199802270-00024.Peer-Reviewed Original ResearchConceptsPosttransplant Kaposi's sarcomaRenal transplant patientsKaposi's sarcomaTransplant patientsSmall viral capsid antigenRenal transplant recipientsTertiary care hospitalViral capsid antigenCross-sectional studyHHV8 antigensSerologic associationsTransplant recipientsRenal failureCare hospitalCapsid antigenCommon cancerControl subjectsSaudi ArabiaSeroreactivity ratesKS patientsSerologic resultsControl groupControl individualsPatientsSarcoma
1990
Inhibition of specific binding of EBNA 1 to DNA by Murine Monoclonal and certain human polyclonal antibodies
Orlowski R, Polvino-Bodnar M, Hearing J, Miller G. Inhibition of specific binding of EBNA 1 to DNA by Murine Monoclonal and certain human polyclonal antibodies. Virology 1990, 176: 638-642. PMID: 2161154, DOI: 10.1016/0042-6822(90)90036-q.Peer-Reviewed Original ResearchConceptsEBNA-1 proteinGel mobility shift experimentsMobility shift experimentsSequence-specific DNARecognition of DNAEBNA-1Binding of DNASpecific bindingLac promoterDNA bindingMonoclonal antibodiesDimerization siteSpecific DNANonfusion proteinBurkitt's lymphoma cellsShift experimentsMolecular massEscherichia coliProteinDNASame monoclonal antibodyBindingPolyclonal antibodiesEB virusLymphoma cellsTopological Effects of EBNA 1 on oriP
Orlowski R, Miller G. Topological Effects of EBNA 1 on oriP. Advances In Experimental Medicine And Biology 1990, 278: 115-124. PMID: 1963028, DOI: 10.1007/978-1-4684-5853-4_12.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, ViralBase SequenceBinding SitesDeoxyribonuclease IV (Phage T4-Induced)DNA, SuperhelicalDNA, ViralEndodeoxyribonucleasesEpstein-Barr Virus Nuclear AntigensEscherichia coliEscherichia coli ProteinsGenes, ViralHerpesvirus 4, HumanMolecular Sequence DataNucleic Acid ConformationPlasmidsRecombinant ProteinsRepetitive Sequences, Nucleic AcidTransformation, Bacterial
1987
Novel nuclear antigens recognized by human sera in lymphocytes latently infected by Epstein-Barr virus
Rowe D, Farrell P, Miller G. Novel nuclear antigens recognized by human sera in lymphocytes latently infected by Epstein-Barr virus. Virology 1987, 156: 153-162. PMID: 3027963, DOI: 10.1016/0042-6822(87)90446-6.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, ViralCell NucleusEpstein-Barr Virus Nuclear AntigensHerpesvirus 4, HumanHumansLymphocytesMolecular WeightConceptsEBNA seraAntigen complexX50-7Immortalization of lymphocytesEpstein-Barr virusEBNA INovel nuclear antigenIV groupNew antigensNuclear antigenLymphocytesCell clonesWestern immunoblottingSerumCell linesEBVAntigenMolecular weight proteinsHuman serumHigh molecular weight proteinsRajiVirusViral genomeGroupWeight proteins
1985
Identification and expression of a nuclear antigen from the genomic region of the Jijoye strain of Epstein-Barr virus that is missing in its nonimmortalizing deletion mutant, P3HR-1.
Rowe D, Heston L, Metlay J, Miller G. Identification and expression of a nuclear antigen from the genomic region of the Jijoye strain of Epstein-Barr virus that is missing in its nonimmortalizing deletion mutant, P3HR-1. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 7429-7433. PMID: 2997790, PMCID: PMC391358, DOI: 10.1073/pnas.82.21.7429.Peer-Reviewed Original ResearchConceptsChronic active EBV infectionActive EBV infectionEpstein-Barr virusJijoye strainEBV infectionEBV DNACertain patientsP3HR-1Human antiseraVirus strainsNuclear antigenHr-1JijoyeVirus deletion mutantsLtk- cellsMouse Ltk- cellsImmortalization processPatientsGene transferNeoantigensLymphocytesInfectionAntigenAntibody Responses to Two Epstein-Barr Virus Nuclear Antigens Defined by Gene Transfer
Miller G, Grogan E, Fischer D, Niederman J, Schooley R, Henle W, Lenoir G, Liu C. Antibody Responses to Two Epstein-Barr Virus Nuclear Antigens Defined by Gene Transfer. New England Journal Of Medicine 1985, 312: 750-755. PMID: 2983211, DOI: 10.1056/nejm198503213121204.Peer-Reviewed Original ResearchConceptsChronic active EBV infectionActive EBV infectionEBV infectionNuclear antigenEpstein-Barr virus nuclear antigenEpstein-Barr virus DNAEBV-seropositive subjectsAbnormal immune responseAssociation of EBVNorth African patientsAbsence of antibodiesSuch patientsAfrican patientsSerologic studiesImmune responseNasopharyngeal carcinomaLymphoid cellsPatientsViral gene productsSpecific viral gene productsViral mutationsSerologic reactionsAntigenSerum samplesM antigenConstitutive expression of Epstein-Barr virus-encoded RNAs and nuclear antigen during latency and after induction of Epstein-Barr virus replication
Weigel R, Fischer D, Heston L, Miller G. Constitutive expression of Epstein-Barr virus-encoded RNAs and nuclear antigen during latency and after induction of Epstein-Barr virus replication. Journal Of Virology 1985, 53: 254-259. PMID: 2981344, PMCID: PMC255024, DOI: 10.1128/jvi.53.1.254-259.1985.Peer-Reviewed Original ResearchConceptsEpstein-Barr virus-encoded RNAVirus-encoded RNAEpstein-Barr virus replicationNuclear antigenEpstein-Barr virusLatent gene productsEpstein-Barr virus gene productsHR-1 cellsVirus gene productsViral variantsVirus replicationAntigen mRNAAbsence of inductionViral DNAAntigenInductionMethod of inductionPhorbol esterViral polypeptidesViral transcriptsGene productsCytoplasmic abundanceExpressionConstitutive expressionConstitutive levels
1984
Epstein-Barr virus with heterogeneous DNA disrupts latency
Miller G, Rabson M, Heston L. Epstein-Barr virus with heterogeneous DNA disrupts latency. Journal Of Virology 1984, 50: 174-182. PMID: 6321789, PMCID: PMC255597, DOI: 10.1128/jvi.50.1.174-182.1984.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusLatent Epstein-Barr virusEarly antigenRaji cellsViral synthesisX50-7 cellsHet DNABurkitt lymphoma linesActivated virusEBV DNANeonatal lymphocytesEBV replicationEBV sequencesX50-7Restriction endonuclease polymorphismAntigenLymphoma linesVirusRare variantsDefective virusLower ratesSuperinfectionCell spreadBiological effectsCellsRegions of the EBV genome involved in latency and lymphocyte immortalization.
Miller G. Regions of the EBV genome involved in latency and lymphocyte immortalization. Medical Virology 1984, 30: 107-28. PMID: 6087408.Peer-Reviewed Original ResearchAnimalsAntigens, ViralB-LymphocytesCell NucleusCell Transformation, ViralChildChild, PreschoolChromosome DeletionDNA, ViralEpstein-Barr Virus Nuclear AntigensFemaleGenes, ViralHerpesvirus 4, HumanHumansLymphomaMaleRepetitive Sequences, Nucleic AcidRNA, ViralTranscription, GeneticTumor Virus InfectionsVirionVirus Replication
1983
Identification of a rare Epstein-Barr virus variant that enhances early antigen expression in Raji cells.
Rabson M, Heston L, Miller G. Identification of a rare Epstein-Barr virus variant that enhances early antigen expression in Raji cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 2762-2766. PMID: 6302703, PMCID: PMC393908, DOI: 10.1073/pnas.80.9.2762.Peer-Reviewed Original Research
1982
Neutralization of lymphocyte immortalization by different strains of Epstein-Barr virus with a murine monoclonal antibody
Miller G, Heston L, Hoffman G. Neutralization of lymphocyte immortalization by different strains of Epstein-Barr virus with a murine monoclonal antibody. Infection And Immunity 1982, 37: 1028-1031. PMID: 6182100, PMCID: PMC347642, DOI: 10.1128/iai.37.3.1028-1031.1982.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusMurine monoclonal antibodiesMonoclonal antibodiesCases of mononucleosisB95-8 strainBurkitt's lymphoma cell linesPolyclonal lymphomaBlood transfusionEBV strainsAmerican patientsChallenge virusLymphoma cell linesBurkitt's lymphomaLymphocyte immortalizationAfrican childrenHybridoma antibodiesAntigenic differencesAntibodiesMarmoset cellsMononucleosisVirusAntigenic sitesCell linesLymphomaAntigen
1981
Epstein-Barr Viral DNA: Infectivity for Human Placental Cells
Miller G, Grogan E, Heston L, Robinson J, Smith D. Epstein-Barr Viral DNA: Infectivity for Human Placental Cells. Science 1981, 212: 452-455. PMID: 6259735, DOI: 10.1126/science.6259735.Peer-Reviewed Original Research
1975
Differences between laboratory strains of Epstein-Barr virus based on immortalization, abortive infection and interference.
Miller G, Robinson J, Heston L, Lipman M. Differences between laboratory strains of Epstein-Barr virus based on immortalization, abortive infection and interference. IARC Scientific Publications 1975, 395-408. PMID: 190145.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusEarly antigenH virusInitiation of immortalizationAntibody-negative human serumPretreatment of lymphocytesNon-producer cell linesCell linesHuman umbilical cordHuman serumInfectious mononucleosisCarrier cell linesB95-8Umbilical cordBurkitt's lymphomaLymphocyte immortalizationDNA synthesisLaboratory strainsStimulation of DNAHuman lymphocytesLymphocytesBiological behaviorPrimary culturesAbortive infectionAntigen
1974
Differences Between Laboratory Strains of Epstein-Barr Virus Based on Immortalization, Abortive Infection, and Interference
Miller G, Robinson J, Heston L, Lipman M. Differences Between Laboratory Strains of Epstein-Barr Virus Based on Immortalization, Abortive Infection, and Interference. Proceedings Of The National Academy Of Sciences Of The United States Of America 1974, 71: 4006-4010. PMID: 4372601, PMCID: PMC434316, DOI: 10.1073/pnas.71.10.4006.Peer-Reviewed Original ResearchConceptsEB virusEarly antigenH virusInitiation of immortalizationAntibody-negative human serumEpstein-Barr virusPretreatment of lymphocytesCell linesHuman umbilical cordDNA synthesisHuman serumInfectious mononucleosisCarrier cell linesBiologic behaviorB95-8Umbilical cordBurkitt's lymphomaLymphocyte immortalizationNonproducer cell linesHuman lymphocytesLaboratory strainsLymphocytesBiologic activityPrimary culturesAbortive infectionImmortalizing and nonimmortalizing laboratory strains of Epstein-Barr Virus.
Miller G, Robinson J, Heston L. Immortalizing and nonimmortalizing laboratory strains of Epstein-Barr Virus. Cold Spring Harbor Symposia On Quantitative Biology 1974, 39 Pt 2: 773-81. PMID: 169031, DOI: 10.1101/sqb.1974.039.01.089.Peer-Reviewed Original Research
1973
Malignant Lymphoma in Cottontop Marmosets after Inoculation with Epstein-Barr Virus
Shope T, Dechairo D, Miller G. Malignant Lymphoma in Cottontop Marmosets after Inoculation with Epstein-Barr Virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1973, 70: 2487-2491. PMID: 4354852, PMCID: PMC427039, DOI: 10.1073/pnas.70.9.2487.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusMalignant lymphomaImmunosuppressive drugsCottontop marmosetsCell-free Epstein-Barr virusCourse of diseaseCell-free virusHuman malignant lymphomasSarcoma typesVirus antigenAddition of virusLymphomaAutologous cellsTumor formationLatent periodOncogenic capacityOrgan cultureMarmosetsVirusTumorsAntibodiesDrugsAdditional evidenceCellsNeoplasiaProlonged Oropharyngeal Excretion of Epstein–Barr Virus after Infectious Mononucleosis
Miller G, Niederman J, Andrews L. Prolonged Oropharyngeal Excretion of Epstein–Barr Virus after Infectious Mononucleosis. New England Journal Of Medicine 1973, 288: 229-232. PMID: 4345064, DOI: 10.1056/nejm197302012880503.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusThroat washesInfectious mononucleosisEpstein-Barr viral antigensEpstein-Barr virus antibodiesInfectious mononucleosis syndromeUmbilical cord leukocytesOropharyngeal excretionClinical symptomsControl subjectsSerum antibodiesVirus antibodiesViral antigensBlood leukocytesSuch antigensComplement fixationContinuous cell linesFactor presentMononucleosisAntibodiesReference seraPatientsCell linesSyndromeLeukocytes