2022
Rapid Screen for Antiviral T‐Cell Immunity with Nanowire Electrochemical Biosensors
Nami M, Han P, Hanlon D, Tatsuno K, Wei B, Sobolev O, Pitruzzello M, Vassall A, Yosinski S, Edelson R, Reed M. Rapid Screen for Antiviral T‐Cell Immunity with Nanowire Electrochemical Biosensors. Advanced Materials 2022, 34: e2109661. PMID: 35165959, DOI: 10.1002/adma.202109661.Peer-Reviewed Original ResearchConceptsT cell immunitySARS-CoV-2Immune responseHuman T cell immune responseAntiviral T cell immunityPathogen-specific T cellsT cell immune responsesT cell analysisPatient's immune responseT cell responsesAntibody-based protectionPandemic SARS-CoV-2Protective immunityT cellsVaccine formulationsB cellsVaccine designBroad protectionDisease riskInfectious diseasesCare toolsTranslational platformImmunityEmergent variantsPandemic coronavirus
2019
Ex vivo dendritic cell generation—A critical comparison of current approaches
Han P, Hanlon D, Sobolev O, Chaudhury R, Edelson RL. Ex vivo dendritic cell generation—A critical comparison of current approaches. International Review Of Cytology 2019, 349: 251-307. PMID: 31759433, DOI: 10.1016/bs.ircmb.2019.10.003.Peer-Reviewed Original ResearchConceptsDendritic cellsDiscovery of DCsMemory T cell responsesProfessional antigen-presenting cellsAntigen-specific immune responsesDendritic cell generationAntigen-specific immunityT cell responsesAntigen-presenting cellsEx vivo productionMononuclear cell fractionRalph SteinmanDC therapyAutoimmune disordersImmunologic functionDC functionPoor survivalImmunologic roleImmune responsePeripheral tissuesPhysiologic productionAdaptive immunityClinical utilityTherapeutic modulationImmune system
2016
Quantifying in vivo murine antigen-specific T cell responses without requirement for prior knowledge of antigen identity
Kibbi N, Hong E, Ezaldein H, Hanlon D, Fahmy T, Edelson R. Quantifying in vivo murine antigen-specific T cell responses without requirement for prior knowledge of antigen identity. Transfusion And Apheresis Science 2016, 56: 179-189. PMID: 28007431, DOI: 10.1016/j.transci.2016.11.004.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaExtracorporeal photochemotherapyCalcium fluxT cellsAntigen-specific T cell responsesMalignant cellsPatient-specific tumor antigensOVA-specific T cellsAntigen-specific T cellsAntigen-specific T cell activationControl recipient micePeptide-loaded DCImmune-based therapiesAnti-tumor responseT cell responsesAnti-cancer immunotherapyT-cell lymphomaT cell engagementT cell activationT cell receptor engagementPatient-specific responsesAdoptive transferClinical responseLymph nodesPeripheral blood
2015
Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*
Hong E, Usiskin IM, Bergamaschi C, Hanlon DJ, Edelson RL, Justesen S, Pavlakis GN, Flavell RA, Fahmy TM. Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*. Journal Of Biological Chemistry 2015, 291: 8931-8950. PMID: 26719339, PMCID: PMC4861462, DOI: 10.1074/jbc.m115.695304.Peer-Reviewed Original ResearchConceptsT cell responsesArtificial antigen-presenting cellsDendritic cellsIL-15Antigen-presenting cellsIL-15RαCell responsesAntigen-specific T cell responsesAntigen-processing dendritic cellsMaximal T cell responsesAnti-tumor immunitySame dendritic cellOptimal immune responseIL-15 functionsMechanism of actionIL-2Antigen deliveryImmune responseDC surfaceParacrine fashionTumor progressionMurine melanomaCellular mechanismsAggressive modelEnhanced potency
2011
Enhanced Stimulation of Anti‐Ovarian Cancer CD8+ T Cells by Dendritic Cells Loaded with Nanoparticle Encapsulated Tumor Antigen
Hanlon DJ, Aldo PB, Devine L, Alvero AB, Engberg AK, Edelson R, Mor G. Enhanced Stimulation of Anti‐Ovarian Cancer CD8+ T Cells by Dendritic Cells Loaded with Nanoparticle Encapsulated Tumor Antigen. American Journal Of Reproductive Immunology 2011, 65: 597-609. PMID: 21241402, PMCID: PMC3082607, DOI: 10.1111/j.1600-0897.2010.00968.x.Peer-Reviewed Original ResearchMeSH KeywordsAntigen PresentationAntigens, DifferentiationAntigens, NeoplasmCarcinomaCD8-Positive T-LymphocytesCells, CulturedCytokinesDendritic CellsFemaleHumansImmunotherapy, AdoptiveLactic AcidLymphocyte ActivationNanoparticlesOvarian NeoplasmsPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerConceptsTumor-associated antigensT cell responsesT cellsDendritic cellsCytokine productionTumor antigensAnti-tumor T cell responsesCell surface co-stimulatory moleculesCD8 T cell responsesSurface co-stimulatory moleculesAnti-tumor immune responseAntigen-loaded DCsTumor lysate antigensCD8 T cellsCo-stimulatory moleculesT cell expressionHuman DCsActivation markersTumor lysateImmune responseLysate antigenBlood monocytesClinical testingEnhanced stimulationAntigen
2010
Polymer nanoparticles containing tumor lysates as antigen delivery vehicles for dendritic cell–based antitumor immunotherapy
Prasad S, Cody V, Saucier-Sawyer JK, Saltzman WM, Sasaki CT, Edelson RL, Birchall MA, Hanlon DJ. Polymer nanoparticles containing tumor lysates as antigen delivery vehicles for dendritic cell–based antitumor immunotherapy. Nanomedicine Nanotechnology Biology And Medicine 2010, 7: 1-10. PMID: 20692374, PMCID: PMC3073408, DOI: 10.1016/j.nano.2010.07.002.Peer-Reviewed Original ResearchConceptsTumor-associated antigensDendritic cellsTumor lysateAntigen presentationT cellsAntigen deliveryNeck squamous carcinoma cell linesPoly (lactic-co-glycolic acid) (PLGA) NPsAnti-tumor CD8Solid organ malignanciesIL-10 productionT cell responsesAnti-tumor vaccinesSquamous carcinoma cell linesNeck cancer cell linesAntigen delivery vehiclesCell linesAdvanced HNSCCAutologous CD8Cancer cell linesOrgan malignanciesAntitumor immunotherapyCarcinoma cell linesImmune mechanismsMonocyte precursors
2005
Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles
Shen H, Ackerman AL, Cody V, Giodini A, Hinson ER, Cresswell P, Edelson RL, Saltzman WM, Hanlon DJ. Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles. Immunology 2005, 117: 78-88. PMID: 16423043, PMCID: PMC1782199, DOI: 10.1111/j.1365-2567.2005.02268.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationBiocompatible MaterialsBiodegradation, EnvironmentalB-LymphocytesCell LineCross-PrimingDendritic CellsEndosomesHistocompatibility Antigens Class IIHumansLactic AcidLymphocyte ActivationMiceMice, Inbred C57BLNanostructuresOvalbuminPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersSerum Albumin, BovineT-LymphocytesConceptsBone marrow-derived dendritic cellsMHC class I presentationAntigen-presenting cellsClass I presentationMHC class IExogenous antigensDendritic cellsClass IAntigen deliveryPrimary mouse bone marrow-derived dendritic cellsSoluble antigenMouse bone marrow-derived dendritic cellsMarrow-derived dendritic cellsProfessional antigen-presenting cellsMajor histocompatibility complex class IProtein-based vaccinationT cell responsesClassic MHC class IExogenous antigen presentationHistocompatibility complex class IAntigen-coated latex beadsCell-associated antigensInterleukin-2 secretionComplex class IEfficiency of presentation