2019
Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy
Hanlon D, Sobolev O, Han P, Ventura A, Vassall A, Kibbi N, Yurter A, Robinson E, Filler R, Tatsuno K, Edelson RL. Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy. Methods In Molecular Biology 2019, 2097: 173-195. PMID: 31776926, DOI: 10.1007/978-1-0716-0203-4_11.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsAntigen-presenting cellsDendritic cellsTumor-associated antigensBlood monocytesDC populationsExtracorporeal photochemotherapyCytokine-derived DCsSource of DCDendritic antigen-presenting cellsHuman peripheral blood mononuclear cellsFunctional antigen-presenting cellsVivo T cell stimulationBlood mononuclear cellsMurine bone marrow precursorsApoptotic tumor cellsT cell stimulationBone marrow precursorsCancer immunotherapyDC differentiationMononuclear cellsIL-4Clinical vaccinationMonocyte activationSupraphysiologic concentrations
2005
Generation of dendritic cells from rabbit bone marrow mononuclear cell cultures supplemented with hGM-CSF and hIL-4
Cody V, Shen H, Shlyankevich M, Tigelaar RE, Brandsma JL, Hanlon DJ. Generation of dendritic cells from rabbit bone marrow mononuclear cell cultures supplemented with hGM-CSF and hIL-4. Veterinary Immunology And Immunopathology 2005, 103: 163-172. PMID: 15621303, DOI: 10.1016/j.vetimm.2004.08.022.Peer-Reviewed Original ResearchConceptsBone marrow mononuclear cellsDendritic cellsDC vaccinesAllogeneic T cell proliferationCottontail rabbit papillomavirus modelDownregulation of CD14Therapeutic vaccination strategiesUseful preclinical dataMononuclear cell culturesT cell proliferationMHC class IIRabbit bone marrow mononuclear cellsMarrow mononuclear cellsRelevant animal modelsCytokines GM-CSFDC vaccinationCervical cancerLymphocyte reactionHuman papillomavirusMononuclear cellsPreclinical dataIL-4Vaccination strategiesNon-specific esterase activityAnimal models