2020
Complement activated interferon-γ-primed human endothelium transpresents interleukin-15 to CD8+ T cells
Xie CB, Jiang B, Qin L, Tellides G, Kirkiles-Smith NC, Jane-wit D, Pober JS. Complement activated interferon-γ-primed human endothelium transpresents interleukin-15 to CD8+ T cells. Journal Of Clinical Investigation 2020, 130: 3437-3452. PMID: 32191642, PMCID: PMC7324183, DOI: 10.1172/jci135060.Peer-Reviewed Original ResearchConceptsIL-15/IL-15Rα complexesIL-1βHuman endothelial cellsMembrane attack complexEndothelial cellsAcute rejectionT cellsT cell-mediated acute rejectionCell-mediated acute rejectionComplement-mediated pathologiesIL-15Rα expressionGraft endothelial cellsHuman coronary artery graftsEffector memory CD4T cell infiltrationCoronary artery graftsIL-1 receptorActive IL-1βCultured human endothelial cellsNLRP3 inflammasome assemblyNoncanonical NF-κBArtery graftAlloreactive CD8Complement membrane attack complexMemory CD4
2019
Complement Membrane Attack Complexes Assemble NLRP3 Inflammasomes Triggering IL-1 Activation of IFN-γ–Primed Human Endothelium
Xie CB, Qin L, Li G, Fang C, Kirkiles-Smith NC, Tellides G, Pober JS, Jane-Wit D. Complement Membrane Attack Complexes Assemble NLRP3 Inflammasomes Triggering IL-1 Activation of IFN-γ–Primed Human Endothelium. Circulation Research 2019, 124: 1747-1759. PMID: 31170059, PMCID: PMC6557295, DOI: 10.1161/circresaha.119.314845.Peer-Reviewed Original ResearchConceptsMembrane attack complexEndothelial cellsComplement membrane attack complexIL-1βNLRP3 inflammasomeEC immunogenicityComplement activationAntibody-mediated complement activationInflammasome assemblyComplement-mediated pathologiesRenal allograft biopsiesGraft endothelial cellsHuman coronary artery graftsLate allograft failureCoronary artery graftsT cell responsesImmune-mediated pathologyActivate endothelial cellsIL-1 receptorIL-1 synthesisIL-1β secretionNoncanonical NF-κB signalingNF-κB signalingAttack complexImmunodeficient mouse hosts
2018
Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1
Liu R, Merola J, Manes TD, Qin L, Tietjen GT, López-Giráldez F, Broecker V, Fang C, Xie C, Chen PM, Kirkiles-Smith NC, Jane-Wit D, Pober JS. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight 2018, 3: e97881. PMID: 29515027, PMCID: PMC5922286, DOI: 10.1172/jci.insight.97881.Peer-Reviewed Original ResearchMeSH KeywordsAllograftsAnimalsAntigen PresentationCell CommunicationCells, CulturedDisease Models, AnimalEndothelial CellsEndothelium, VascularFemaleGraft RejectionHealthy VolunteersHuman Umbilical Vein Endothelial CellsHumansIndoleamine-Pyrrole 2,3,-DioxygenaseInterferon-gammaIsoantigensMice, SCIDMicrovesselsPericytesPrimary Cell CultureRNA, Small InterferingSkinSkin TransplantationT-Lymphocytes, CytotoxicTransplantation ChimeraTransplantation, HomologousTryptophanConceptsInduction of indoleamineHuman pericytesEndothelial cellsAllograft rejectionTryptophan depletionT cellsAcute T cell-mediated rejectionT cell-mediated rejectionEffector memory T cellsDioxygenase 1Early acute rejectionCell-mediated rejectionSkin allograft rejectionAlloreactive T cellsHuman renal allograftsMemory T cellsRole of ECsContribution of pericytesAcute rejectionRenal allograftsImmunoregulatory effectsImmunosuppressive propertiesHuman allograftsMouse modelMicrovascular pericytes