2019
Critical role of Lin28‐TNFR2 signalling in cardiac stem cell activation and differentiation
Xiang Q, Yang B, Li L, Qiu B, Qiu C, Gao X, Zhou H, Min W. Critical role of Lin28‐TNFR2 signalling in cardiac stem cell activation and differentiation. Journal Of Cellular And Molecular Medicine 2019, 23: 0-0. PMID: 30734494, PMCID: PMC6433861, DOI: 10.1111/jcmm.14202.Peer-Reviewed Original ResearchConceptsCardiac stem cell activationStem cell activationHuman inducible pluripotent stem cellsCardiac stem cell differentiationCSC activationStem cell differentiationInducible pluripotent stem cellsPluripotent stem cellsCardiac progenitor cellsCritical roleActivation of TNFR2Factor RNACell activationProtein Lin28Cardiomyocyte proteinsCell differentiationStem cellsProgenitor cellsStem cell-based therapiesCSC differentiationProtein expressionDifferentiationCell-based therapiesExpressionActivation
2018
Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells
Ali M, Khan SY, Vasanth S, Ahmed MR, Chen R, Na CH, Thomson JJ, Qiu C, Gottsch JD, Riazuddin SA. Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science 2018, 59: 2437-2444. PMID: 29847650, PMCID: PMC5957521, DOI: 10.1167/iovs.17-22927.Peer-Reviewed Original ResearchMeSH KeywordsAgedCell DifferentiationCells, CulturedCryopreservationEmbryonic Stem CellsEndothelium, CornealFlow CytometryGene Expression ProfilingGenetic MarkersHumansImmunohistochemistryInduced Pluripotent Stem CellsLeukocytes, MononuclearMaleMass SpectrometryMicroscopy, Phase-ContrastMiddle AgedNeural CrestProteomeReal-Time Polymerase Chain ReactionConceptsNeural crest cellsProteome sequencingProteome profilingCorneal endothelial cellsExpression of pluripotentQuantitative real-time PCRPluripotent stem cellsMolecular architectureCrest cellsEndothelial cellsProteomeReal-time PCRPluripotency markersHuman corneal endotheliumStem cellsPhase contrast microscopyExpression levelsProteinIPSCsSequencingCellsProfilingFirst reportContrast microscopyHigh levels
2016
Tissue-Engineered Vascular Rings from Human iPSC-Derived Smooth Muscle Cells
Dash BC, Levi K, Schwan J, Luo J, Bartulos O, Wu H, Qiu C, Yi T, Ren Y, Campbell S, Rolle MW, Qyang Y. Tissue-Engineered Vascular Rings from Human iPSC-Derived Smooth Muscle Cells. Stem Cell Reports 2016, 7: 19-28. PMID: 27411102, PMCID: PMC4945325, DOI: 10.1016/j.stemcr.2016.05.004.Peer-Reviewed Original ResearchConceptsVascular tissue engineeringFunctional vascular smooth muscle cellsCell-based tissueSelf-assembly approachRenewable sourcesTissue engineeringPluripotent stem cellsPlatform technologyBiomedical applicationsTissue ringsDrug screeningDisease modelingTissue model systemsHuman iPSCStem cellsBroad utilityEfficient approachLarge quantitiesEngineeringMaterials
2015
Characterization of the mammalian miRNA turnover landscape
Guo Y, Liu J, Elfenbein SJ, Ma Y, Zhong M, Qiu C, Ding Y, Lu J. Characterization of the mammalian miRNA turnover landscape. Nucleic Acids Research 2015, 43: 2326-2341. PMID: 25653157, PMCID: PMC4344502, DOI: 10.1093/nar/gkv057.Peer-Reviewed Original ResearchConceptsMiRNA turnoverStable small RNAsMammalian cell typesCultured mammalian cellsSubset of miRNAsTurnover kineticsMiRNA biogenesisMost miRNAsMiR-222-5pNucleotide biasSmall RNAsMiRNA maturationMammalian cellsSame miRNAMiRNA poolExpression profilingHsp90 associationSequence determinantsDeep sequencingHsp90 inhibitionTurnover rateMiRNA isoformsDifferent turnover ratesSequence featuresCell types
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina
2012
High‐Efficiency Transfection and siRNA‐Mediated Gene Knockdown in Human Pluripotent Stem Cells
Ma Y, Lin H, Qiu C. High‐Efficiency Transfection and siRNA‐Mediated Gene Knockdown in Human Pluripotent Stem Cells. Current Protocols In Stem Cell Biology 2012, 21: 5c.2.1-5c.2.9. PMID: 22605647, DOI: 10.1002/9780470151808.sc05c02s21.Peer-Reviewed Original ResearchConceptsHigh transfection efficiencyPluripotent stem cellsTransfection efficiencyTransfection reagent Lipofectamine 2000Human embryonic stem cellsStem cellsHuman pluripotent stem cellsEmbryonic stem cellsPluripotent cell linesLipofectamine 2000SiRNA-mediated gene knockdownPluripotent cellsExpensive equipmentPluripotent genesGene knockdownProtocolEfficiencyPrimary cellsGenesCell linesCarriersCellsApplicationsTransfection
2010
High-efficiency siRNA-based gene knockdown in human embryonic stem cells
Ma Y, Jin J, Dong C, Cheng EC, Lin H, Huang Y, Qiu C. High-efficiency siRNA-based gene knockdown in human embryonic stem cells. RNA 2010, 16: 2564-2569. PMID: 20978109, PMCID: PMC2995416, DOI: 10.1261/rna.2350710.Peer-Reviewed Original ResearchDynamic transcriptomes during neural differentiation of human embryonic stem cells revealed by short, long, and paired-end sequencing
Wu JQ, Habegger L, Noisa P, Szekely A, Qiu C, Hutchison S, Raha D, Egholm M, Lin H, Weissman S, Cui W, Gerstein M, Snyder M. Dynamic transcriptomes during neural differentiation of human embryonic stem cells revealed by short, long, and paired-end sequencing. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 5254-5259. PMID: 20194744, PMCID: PMC2841935, DOI: 10.1073/pnas.0914114107.Peer-Reviewed Original ResearchConceptsNeural differentiationUndifferentiated hESCsNeural fate specificationCell identity maintenanceStage-specific regulationHuman embryonic stem cellsTypes of genesPaired-end sequencingDifferentiation of hESCsEmbryonic stem cellsPaired-end readsNeural cell differentiationSplicing dynamicsFate specificationDynamic transcriptomeIsoform diversityTranscriptome changesUnannotated transcriptsGene transcriptionRNA sequencingStages of differentiationNeural lineagesCell differentiationDifferential expressionGliogenic potential
2009
Complex developmental patterns of histone modifications associated with the human β-globin switch in primary cells
Hsu M, Richardson CA, Olivier E, Qiu C, Bouhassira EE, Lowrey CH, Fiering S. Complex developmental patterns of histone modifications associated with the human β-globin switch in primary cells. Experimental Hematology 2009, 37: 799-806.e4. PMID: 19460472, PMCID: PMC2748252, DOI: 10.1016/j.exphem.2009.04.006.Peer-Reviewed Original ResearchMeSH KeywordsBeta-GlobinsCells, CulturedChromatin ImmunoprecipitationHistonesHumansPolymerase Chain ReactionConceptsHistone modificationsGene expressionBeta-globin switchDimethyl lysine 9Beta-globin locusΒ-globin switchGamma geneImportant histone modificationsBone marrow cellsGamma gene expressionSuch histonesComplex developmental patternLysine 9Chromatin immunoprecipitationHistone H3Gene suppressionPrimary fetalMarrow cellsUnexpressed genesGamma-globinGenesHistonesDevelopmental stagesBone marrow erythroblastsPrimary cellsRole for MKL1 in megakaryocytic maturation
Cheng EC, Luo Q, Bruscia EM, Renda MJ, Troy JA, Massaro SA, Tuck D, Schulz V, Mane SM, Berliner N, Sun Y, Morris SW, Qiu C, Krause DS. Role for MKL1 in megakaryocytic maturation. Blood 2009, 113: 2826-2834. PMID: 19136660, PMCID: PMC2661865, DOI: 10.1182/blood-2008-09-180596.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Cell CountBone MarrowCell DifferentiationCell Line, TumorCells, CulturedDNA-Binding ProteinsGene Expression ProfilingGene Expression RegulationHumansLeukemia, Erythroblastic, AcuteMegakaryocytesMiceMice, Inbred C57BLMice, KnockoutOligonucleotide Array Sequence AnalysisOncogene Proteins, FusionPloidiesRecombinant Fusion ProteinsRNA InterferenceRNA, Small InterferingSerum Response FactorThrombocytopeniaThrombopoiesisThrombopoietinTrans-ActivatorsConceptsMegakaryoblastic leukemia 1Reduced platelet countsSerum response factorMegakaryocytic differentiationPeripheral bloodPlatelet countMKL1 expressionMegakaryoblastic leukemiaBone marrow megakaryocytesMuscle cellsPresence of thrombopoietinPhysiologic maturationHuman erythroleukemia cell lineIncreased numberMarrow megakaryocytesCell linesErythroleukemia cell lineMegakaryocytesMegakaryocytic maturationDifferentiated muscle cellsOverexpressionConcurrent increaseMuscle differentiationCellsMaturation