2022
The role of FYCO1-dependent autophagy in lens fiber cell differentiation
Khan SY, Ali M, Kabir F, Na CH, Delannoy M, Ma Y, Qiu C, Costello MJ, Hejtmancik JF, Riazuddin SA. The role of FYCO1-dependent autophagy in lens fiber cell differentiation. Autophagy 2022, 18: 2198-2215. PMID: 35343376, PMCID: PMC9397473, DOI: 10.1080/15548627.2022.2025570.Peer-Reviewed Original ResearchConceptsAutophagic vesiclesLens fiber cell differentiationMouse lensesAutophagic fluxFiber cell differentiationHuman embryonic stem cellsCoiled-coil domainOrganelle-free zoneEmbryonic stem cellsHuman lens epithelial cellsAutophagy-associated genesSingle guide RNAsQuantitative real-time PCRLens epithelial cellsAdaptor proteinRNA-seqGuide RNARNA sequencingCellular organellesLens morphogenesisCataract phenotypeWild typeFYCO1Cell differentiationEndoplasmic reticulum
2019
Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies
Ali M, Kabir F, Thomson JJ, Ma Y, Qiu C, Delannoy M, Khan SY, Riazuddin SA. Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies. Scientific Reports 2019, 9: 18552. PMID: 31811247, PMCID: PMC6898283, DOI: 10.1038/s41598-019-54258-z.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsComparative transcriptome analysisTranscriptome analysisLentoid bodiesPluripotent stem cellsBody transcriptomeRNA sequencingStem cellsNext-generation RNA sequencingEmbryonic stem cellsFiber-like cellsSimilar expression profilesTranscriptome datasetsTranscriptome profilingCell transcriptomeLens morphogenesisExcellent systemMouse lensExpression profilesTranscriptomeMechanism of cataractogenesisLens-like structuresUltrastructure analysisGenesOcular lens
2010
Dynamic transcriptomes during neural differentiation of human embryonic stem cells revealed by short, long, and paired-end sequencing
Wu JQ, Habegger L, Noisa P, Szekely A, Qiu C, Hutchison S, Raha D, Egholm M, Lin H, Weissman S, Cui W, Gerstein M, Snyder M. Dynamic transcriptomes during neural differentiation of human embryonic stem cells revealed by short, long, and paired-end sequencing. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 5254-5259. PMID: 20194744, PMCID: PMC2841935, DOI: 10.1073/pnas.0914114107.Peer-Reviewed Original ResearchConceptsNeural differentiationUndifferentiated hESCsNeural fate specificationCell identity maintenanceStage-specific regulationHuman embryonic stem cellsTypes of genesPaired-end sequencingDifferentiation of hESCsEmbryonic stem cellsPaired-end readsNeural cell differentiationSplicing dynamicsFate specificationDynamic transcriptomeIsoform diversityTranscriptome changesUnannotated transcriptsGene transcriptionRNA sequencingStages of differentiationNeural lineagesCell differentiationDifferential expressionGliogenic potential