Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells
Ali M, Khan SY, Vasanth S, Ahmed MR, Chen R, Na CH, Thomson JJ, Qiu C, Gottsch JD, Riazuddin SA. Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science 2018, 59: 2437-2444. PMID: 29847650, PMCID: PMC5957521, DOI: 10.1167/iovs.17-22927.Peer-Reviewed Original ResearchMeSH KeywordsAgedCell DifferentiationCells, CulturedCryopreservationEmbryonic Stem CellsEndothelium, CornealFlow CytometryGene Expression ProfilingGenetic MarkersHumansImmunohistochemistryInduced Pluripotent Stem CellsLeukocytes, MononuclearMaleMass SpectrometryMicroscopy, Phase-ContrastMiddle AgedNeural CrestProteomeReal-Time Polymerase Chain ReactionConceptsNeural crest cellsProteome sequencingProteome profilingCorneal endothelial cellsExpression of pluripotentQuantitative real-time PCRPluripotent stem cellsMolecular architectureCrest cellsEndothelial cellsProteomeReal-time PCRPluripotency markersHuman corneal endotheliumStem cellsPhase contrast microscopyExpression levelsProteinIPSCsSequencingCellsProfilingFirst reportContrast microscopyHigh levels