2024
Upper respiratory Streptococcus pneumoniae colonization among working-age adults with prevalent exposure to overcrowding
Parker A, Jackson N, Awasthi S, Kim H, Alwan T, Wyllie A, Kogut K, Holland N, Mora A, Eskenazi B, Riley L, Lewnard J. Upper respiratory Streptococcus pneumoniae colonization among working-age adults with prevalent exposure to overcrowding. Microbiology Spectrum 2024, 12: e00879-24. PMID: 39012111, PMCID: PMC11302326, DOI: 10.1128/spectrum.00879-24.Peer-Reviewed Original ResearchPneumococcal carriageStreptococcus pneumoniae</i>Pneumococcal diseaseLt;5 yearsPredictors of carriagePrecursor to pneumococcal diseaseAssociated with increased riskRisk of carriageExposure to childrenYoung childrenExposure to young childrenCycle threshold valuesPneumococcal densityVulnerable age groupRisk factorsPneumococcal transmissionIncreased prevalenceNon-carriersPneumococciAge groupsCarriageSaliva samplesAdult populationMilitary recruitsQuantitative PCR assay
2023
649. Expansion Of A Low-Cost, Open-Source, Saliva-Based PCR Test For The Detection Of Mpox Virus
Thomas R, Yolda-Carr D, Steel S, Tobik E, Zepeda T, Brownlee M, Saladi S, Parkin J, Fajardo K, Allicock O, Wyllie A. 649. Expansion Of A Low-Cost, Open-Source, Saliva-Based PCR Test For The Detection Of Mpox Virus. Open Forum Infectious Diseases 2023, 10: ofad500.712. PMCID: PMC10677374, DOI: 10.1093/ofid/ofad500.712.Peer-Reviewed Original Research
2020
Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
Kudo E, Israelow B, Vogels CBF, Lu P, Wyllie AL, Tokuyama M, Venkataraman A, Brackney DE, Ott IM, Petrone ME, Earnest R, Lapidus S, Muenker MC, Moore AJ, Casanovas-Massana A, Team Y, Omer SB, Dela Cruz CS, Farhadian SF, Ko AI, Grubaugh ND, Iwasaki A. Detection of SARS-CoV-2 RNA by multiplex RT-qPCR. PLOS Biology 2020, 18: e3000867. PMID: 33027248, PMCID: PMC7571696, DOI: 10.1371/journal.pbio.3000867.Peer-Reviewed Original ResearchMeSH KeywordsBetacoronavirusCase-Control StudiesClinical Laboratory TechniquesCoronavirus InfectionsCOVID-19COVID-19 TestingDNA PrimersHEK293 CellsHumansLimit of DetectionMultiplex Polymerase Chain ReactionNasopharynxPandemicsPneumonia, ViralReagent Kits, DiagnosticReverse Transcriptase Polymerase Chain ReactionRNA, ViralSARS-CoV-2United StatesConceptsSARS-CoV-2 RNAMultiplex RT-qPCRRT-qPCRSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testingSARS-CoV-2Quantitative reverse transcription PCRCycle threshold valuesReverse transcription-PCRRT-qPCR assaysDisease controlMultiplex RT-qPCR assayTranscription-PCRAssaysSingle assayLow copy number