2024
Prior Influenza Infection Mitigates SARS-CoV-2 Disease in Syrian Hamsters
Di Pietro C, Haberman A, Lindenbach B, Smith P, Bruscia E, Allore H, Vander Wyk B, Tyagi A, Zeiss C. Prior Influenza Infection Mitigates SARS-CoV-2 Disease in Syrian Hamsters. Viruses 2024, 16: 246. PMID: 38400021, PMCID: PMC10891789, DOI: 10.3390/v16020246.Peer-Reviewed Original ResearchConceptsTransient gene expressionSARS-CoV-2Viral replication pathwayReplication pathwayAntiviral pathwaysEndemism patternsUpregulation of innateGene expressionQuantitative RT-PCRMitigated weight lossDual-infected animalsSARS-CoV-2 viral loadSARS-CoV-2 infectionSyrian hamstersSeasonal infection ratesSARS-CoV-2 inoculationLungs of animalsIndividual virusesSARS-CoV-2 diseaseUpper respiratory tractH1N1 infectionRT-PCRBronchoalveolar lavageViral loadCytokine levelsIntracellular calcium links milk stasis to lysosome-dependent cell death during early mammary gland involution
Jeong J, Lee J, Talaia G, Kim W, Song J, Hong J, Yoo K, Gonzalez D, Athonvarangkul D, Shin J, Dann P, Haberman A, Kim L, Ferguson S, Choi J, Wysolmerski J. Intracellular calcium links milk stasis to lysosome-dependent cell death during early mammary gland involution. Cellular And Molecular Life Sciences 2024, 81: 29. PMID: 38212474, PMCID: PMC10784359, DOI: 10.1007/s00018-023-05044-8.Peer-Reviewed Original Research
2019
In vivo dynamics of T cells and their interactions with dendritic cells in mouse cutaneous graft-versus-host disease
Morin-Zorman S, Wysocki C, Zhu J, Li H, Zorman S, Matte-Martone C, Kisanga E, McNiff J, Jain D, Gonzalez D, Rothstein DM, Lakkis FG, Haberman A, Shlomchik WD. In vivo dynamics of T cells and their interactions with dendritic cells in mouse cutaneous graft-versus-host disease. Blood Advances 2019, 3: 2082-2092. PMID: 31296496, PMCID: PMC6650737, DOI: 10.1182/bloodadvances.2019000227.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCD11c AntigenCell CommunicationDendritic CellsDisease Models, AnimalFluorescent Antibody TechniqueGene ExpressionGenes, ReporterGraft vs Host DiseaseHematopoietic Stem Cell TransplantationHistocompatibility Antigens Class IImmunophenotypingLymphocyte DepletionMiceMice, TransgenicProtein BindingReceptors, Antigen, T-CellSkin DiseasesT-Lymphocyte SubsetsT-LymphocytesTransplantation, HomologousConceptsDonor dendritic cellsCD8 cellsT cellsGVHD lesionsHost diseaseCD4 cellsDendritic cellsAllogeneic hematopoietic stem cell transplantationHematopoietic stem cell transplantationMajority of CD4Dynamics of CD4Stem cell transplantationCutaneous graftAntibody infusionCell transplantationMyeloid cellsIntravital microscopyAcute deletionMajor causeLesionsTarget tissuesCD103CD4InfusionMHCIIGerminal center B cell initiation, GC maturation, and the coevolution of its stromal cell niches
Haberman AM, Gonzalez DG, Wong P, Zhang T, Kerfoot SM. Germinal center B cell initiation, GC maturation, and the coevolution of its stromal cell niches. Immunological Reviews 2019, 288: 10-27. PMID: 30874342, PMCID: PMC10234181, DOI: 10.1111/imr.12731.Peer-Reviewed Original Research
2018
Nonredundant Roles of IL-21 and IL-4 in the Phased Initiation of Germinal Center B Cells and Subsequent Self-Renewal Transitions.
Gonzalez DG, Cote CM, Patel JR, Smith CB, Zhang Y, Nickerson KM, Zhang T, Kerfoot SM, Haberman AM. Nonredundant Roles of IL-21 and IL-4 in the Phased Initiation of Germinal Center B Cells and Subsequent Self-Renewal Transitions. The Journal Of Immunology 2018, 201: 3569-3579. PMID: 30446568, PMCID: PMC6289626, DOI: 10.4049/jimmunol.1500497.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisB-LymphocytesCell DifferentiationCell Self RenewalCells, CulturedGerminal CenterInterleukin-4InterleukinsLymphocyte ActivationMiceMice, KnockoutParacrine CommunicationProto-Oncogene Proteins c-bcl-6Receptors, Interleukin-21Signal TransductionSTAT6 Transcription FactorT-Lymphocytes, Helper-InducerConceptsIL-21IL-4B cellsIL-21RCytokine IL-21Costimulatory molecule expressionB cell folliclesIL-21 signalingGC B cell developmentNonredundant roleGerminal center B cellsMurine model systemGC B cellsMolecule expressionPhenotypic maturationB cell developmentCell expressionCytokine signalsB cell transitionCombined absenceSubsequent maturationCell migrationCell developmentHistone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis
Hatzi K, Geng H, Doane AS, Meydan C, LaRiviere R, Cardenas M, Duy C, Shen H, Vidal MNC, Baslan T, Mohammad HP, Kruger RG, Shaknovich R, Haberman AM, Inghirami G, Lowe SW, Melnick AM. Histone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis. Nature Immunology 2018, 20: 86-96. PMID: 30538335, PMCID: PMC6294324, DOI: 10.1038/s41590-018-0273-1.Peer-Reviewed Original Research
2017
Germinal center B cell development has distinctly regulated stages completed by disengagement from T cell help
Zhang TT, Gonzalez DG, Cote CM, Kerfoot SM, Deng S, Cheng Y, Magari M, Haberman AM. Germinal center B cell development has distinctly regulated stages completed by disengagement from T cell help. ELife 2017, 6: e19552. PMID: 28498098, PMCID: PMC5429091, DOI: 10.7554/elife.19552.Peer-Reviewed Original ResearchTissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice
Park S, Gonzalez DG, Guirao B, Boucher JD, Cockburn K, Marsh ED, Mesa KR, Brown S, Rompolas P, Haberman AM, Bellaïche Y, Greco V. Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice. Nature Cell Biology 2017, 19: 155-163. PMID: 28248302, PMCID: PMC5581297, DOI: 10.1038/ncb3472.Peer-Reviewed Original ResearchTissue-scale organizationEpidermal wound repairRegional coexistenceCellular behaviorDifferentiated cellsEpithelial behaviorUndamaged cellsProliferation functionTissue architectureHomeostatic functionsDifferentiation changesLive mammalsLive miceWound repairTissue repairProliferative zoneHomeostatic behaviorCellsSkin repairSpatiotemporal dynamicsMammalsWoundsMiceRepairMigration
2016
Preclinical Advances with Multiphoton Microscopy in Live Imaging of Skin Cancers
Sun TY, Haberman AM, Greco V. Preclinical Advances with Multiphoton Microscopy in Live Imaging of Skin Cancers. Journal Of Investigative Dermatology 2016, 137: 282-287. PMID: 27847119, PMCID: PMC5258832, DOI: 10.1016/j.jid.2016.08.033.Peer-Reviewed Original Research
2015
Intravital imaging of hair follicle regeneration in the mouse
Pineda CM, Park S, Mesa KR, Wolfel M, Gonzalez DG, Haberman AM, Rompolas P, Greco V. Intravital imaging of hair follicle regeneration in the mouse. Nature Protocols 2015, 10: 1116-1130. PMID: 26110716, PMCID: PMC4632978, DOI: 10.1038/nprot.2015.070.Peer-Reviewed Original ResearchNiche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool
Mesa KR, Rompolas P, Zito G, Myung P, Sun TY, Brown S, Gonzalez DG, Blagoev KB, Haberman AM, Greco V. Niche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool. Nature 2015, 522: 94-97. PMID: 25849774, PMCID: PMC4457634, DOI: 10.1038/nature14306.Peer-Reviewed Original Research
2014
The BCL6 RD2 Domain Governs Commitment of Activated B Cells to Form Germinal Centers
Huang C, Gonzalez DG, Cote CM, Jiang Y, Hatzi K, Teater M, Dai K, Hla T, Haberman AM, Melnick A. The BCL6 RD2 Domain Governs Commitment of Activated B Cells to Form Germinal Centers. Cell Reports 2014, 8: 1497-1508. PMID: 25176650, PMCID: PMC4163070, DOI: 10.1016/j.celrep.2014.07.059.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB-LymphocytesCell MovementCytokinesDNA-Binding ProteinsGerminal CenterHistone Deacetylase 2Lymphocyte ActivationMiceMutationProtein BindingProtein Structure, TertiaryProto-Oncogene Proteins c-bcl-6Receptors, G-Protein-CoupledReceptors, LysosphingolipidSphingosine-1-Phosphate ReceptorsT-Lymphocytes, Helper-InducerConceptsInnate immune cell phenotypesGC Tfh cellsImmune cell phenotypesT helper cellsGerminal center formationB cells migrateActivated B cellsB cell migrationHelper cellsInflammatory diseasesInterfollicular zonesTranscriptional repressor functionExtrafollicular responseGerminal centersB cellsImmune systemReceptor S1PR1MiceRepression domainRD2 domainRepressor functionCell migrationCells migrateCenter formationBiochemical specificityDynamic Expression of BCL6 in Murine Conventional Dendritic Cells during In Vivo Development and Activation
Zhang TT, Liu D, Calabro S, Eisenbarth SC, Cattoretti G, Haberman AM. Dynamic Expression of BCL6 in Murine Conventional Dendritic Cells during In Vivo Development and Activation. PLOS ONE 2014, 9: e101208. PMID: 24979752, PMCID: PMC4076320, DOI: 10.1371/journal.pone.0101208.Peer-Reviewed Original ResearchConceptsProtein levelsBCL6 levelsMurine conventional dendritic cellsFollicular helper T cellsCommon DC precursorsFunction of Bcl6BCL6 protein levelsConventional dendritic cellsHelper T cellsSecondary lymphoid organsGerminal center B cellsElevated protein levelsBone marrow hematopoietic stem cellsTranscriptional repressor BCL6Marrow hematopoietic stem cellsAdjuvant inoculationCDC subsetsSplenic cDCsDendritic cellsLPS injectionDC precursorsLymphoid organsMicrobial stimuliT cellsKi-67What ticks do under your skin: two-photon intravital imaging of Ixodes scapularis feeding in the presence of the lyme disease spirochete.
Bockenstedt LK, Gonzalez D, Mao J, Li M, Belperron AA, Haberman A. What ticks do under your skin: two-photon intravital imaging of Ixodes scapularis feeding in the presence of the lyme disease spirochete. The Yale Journal Of Biology And Medicine 2014, 87: 3-13. PMID: 24600332, PMCID: PMC3941458.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArachnid VectorsBorrelia burgdorferiFeeding BehaviorGreen Fluorescent ProteinsHost-Parasite InteractionsHost-Pathogen InteractionsIxodesLyme DiseaseMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalMicroscopy, Electron, ScanningMicroscopy, Fluorescence, MultiphotonNymphSkinTick InfestationsTime Factors
2012
In vivo imaging of virological synapses
Sewald X, Gonzalez DG, Haberman AM, Mothes W. In vivo imaging of virological synapses. Nature Communications 2012, 3: 1320. PMID: 23271654, PMCID: PMC3784984, DOI: 10.1038/ncomms2338.Peer-Reviewed Original ResearchConceptsVirological synapsesHuman T-cell lymphotropic virusMurine leukemia virusHuman immunodeficiency virusLeukemia virusViral envelope glycoproteinsLymph nodesImmunodeficiency virusFriend murine leukemia virusLymphotropic virusB cellsIntravital microscopyCell-cell contactEnvelope glycoproteinSynapsesUninfected cellsIntravital imagingVirusMiceVivoCellsLeucocytesCombination delivery of TGF-β inhibitor and IL-2 by nanoscale liposomal polymeric gels enhances tumour immunotherapy
Park J, Wrzesinski SH, Stern E, Look M, Criscione J, Ragheb R, Jay SM, Demento SL, Agawu A, Licona Limon P, Ferrandino AF, Gonzalez D, Habermann A, Flavell RA, Fahmy TM. Combination delivery of TGF-β inhibitor and IL-2 by nanoscale liposomal polymeric gels enhances tumour immunotherapy. Nature Materials 2012, 11: 895-905. PMID: 22797827, PMCID: PMC3601683, DOI: 10.1038/nmat3355.Peer-Reviewed Original ResearchConceptsInterleukin-2TGF-β inhibitorTumor microenvironmentImmune responseLocal tumor immune responseMultiple immunologic mechanismsT cell infiltrationNatural killer cellsTumor immune responseAdaptive immune responsesTumor-bearing miceImmunotherapy resultsConventional immunotherapyImmunologic mechanismsKiller cellsTumor immunotherapyMetastatic melanomaConventional cytokinesProtein cytokinesHigh dosesTumor growthGrowth factorMolecular inhibitorsCombination deliveryImmunotherapyLive imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration
Rompolas P, Deschene ER, Zito G, Gonzalez DG, Saotome I, Haberman AM, Greco V. Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration. Nature 2012, 487: 496-499. PMID: 22763436, PMCID: PMC3772651, DOI: 10.1038/nature11218.Peer-Reviewed Original ResearchSpirochete antigens persist near cartilage after murine Lyme borreliosis therapy
Bockenstedt LK, Gonzalez DG, Haberman AM, Belperron AA. Spirochete antigens persist near cartilage after murine Lyme borreliosis therapy. Journal Of Clinical Investigation 2012, 122: 2652-2660. PMID: 22728937, PMCID: PMC3386809, DOI: 10.1172/jci58813.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntigens, BacterialArthritis, InfectiousBacterial LoadBorrelia burgdorferiCartilageCeftriaxoneDoxycyclineEar, ExternalFemaleFluorescence Recovery After PhotobleachingGreen Fluorescent ProteinsJoint CapsuleLyme DiseaseMiceMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMicroscopy, Fluorescence, MultiphotonMyeloid Differentiation Factor 88PatellaRecombinant ProteinsConceptsNaive miceAntibiotic treatmentIntravital microscopyAntibiotic-refractory Lyme arthritisLyme diseaseTNF-α productionBorrelia burgdorferi antigensB. burgdorferi antigensSpirochete antigenTLR responsivenessInflammatory arthritisAntibiotic therapyLyme arthritisWT miceMusculoskeletal symptomsAntigens persistSlow resolutionImmunodeficient miceMouse modelTissue transplantsPathogen burdenSpirochete DNAInfectious spirochetesLyme borreliosisMiceProxTom Lymphatic Vessel Reporter Mice Reveal Prox1 Expression in the Adrenal Medulla, Megakaryocytes, and Platelets
Truman LA, Bentley KL, Smith EC, Massaro SA, Gonzalez DG, Haberman AM, Hill M, Jones D, Min W, Krause DS, Ruddle NH. ProxTom Lymphatic Vessel Reporter Mice Reveal Prox1 Expression in the Adrenal Medulla, Megakaryocytes, and Platelets. American Journal Of Pathology 2012, 180: 1715-1725. PMID: 22310467, PMCID: PMC3349900, DOI: 10.1016/j.ajpath.2011.12.026.Peer-Reviewed Original ResearchMeSH KeywordsAdrenal MedullaAnimalsBlood PlateletsCells, CulturedCytoplasmEndothelial CellsGene Expression RegulationGenotypeGlycoproteinsHomeodomain ProteinsLuminescent ProteinsLymph NodesLymphatic VesselsMegakaryocytesMembrane Transport ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, FluorescenceTumor Cells, CulturedTumor Suppressor ProteinsConceptsLymph nodesLymphatic vesselsAdrenal medullaExpression of Prox1Tumor metastasisHigh endothelial venulesProx1 expressionTwo-photon laser scanning microscopyTransplant rejectionDentate gyrusEndothelial venulesAntigen presentationC57BL/6 backgroundTransgenic miceLipid metabolismMiceNeuroendocrine cellsAdult liverNovel siteMetastasisMedullaStudy of diseasesLiving mouseUnknown rolePotential utility
2011
Invariant natural killer T cells direct B cell responses to cognate lipid antigen in an IL-21-dependent manner
King IL, Fortier A, Tighe M, Dibble J, Watts GF, Veerapen N, Haberman AM, Besra GS, Mohrs M, Brenner MB, Leadbetter EA. Invariant natural killer T cells direct B cell responses to cognate lipid antigen in an IL-21-dependent manner. Nature Immunology 2011, 13: 44-50. PMID: 22120118, PMCID: PMC3833037, DOI: 10.1038/ni.2172.Peer-Reviewed Original Research