2019
Id4 Downstream of Notch2 Maintains Neural Stem Cell Quiescence in the Adult Hippocampus
Zhang R, Boareto M, Engler A, Louvi A, Giachino C, Iber D, Taylor V. Id4 Downstream of Notch2 Maintains Neural Stem Cell Quiescence in the Adult Hippocampus. Cell Reports 2019, 28: 1485-1498.e6. PMID: 31390563, DOI: 10.1016/j.celrep.2019.07.014.Peer-Reviewed Original ResearchConceptsNeural stem cellsDentate gyrusNSC quiescenceAdult mouse hippocampal dentate gyrusNSC proliferationMouse hippocampal dentate gyrusAdult dentate gyrusHippocampal dentate gyrusExpense of neurogenesisNeural stem cell quiescenceId4 knockdownAdult hippocampusNeuron generationId4 expressionNeuronal differentiationCell cycle entryNSC activationMajor effectorStem cell quiescenceNotch2NeurogenesisCell quiescenceStem cellsDownstream targetsNSC maintenance
2018
Notch2 Signaling Maintains NSC Quiescence in the Murine Ventricular-Subventricular Zone
Engler A, Rolando C, Giachino C, Saotome I, Erni A, Brien C, Zhang R, Zimber-Strobl U, Radtke F, Artavanis-Tsakonas S, Louvi A, Taylor V. Notch2 Signaling Maintains NSC Quiescence in the Murine Ventricular-Subventricular Zone. Cell Reports 2018, 22: 992-1002. PMID: 29386140, DOI: 10.1016/j.celrep.2017.12.094.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationLateral VentriclesMiceNeural Stem CellsReceptor, Notch2Signal TransductionConceptsV-SVZ neural stem cellsVentricular-subventricular zoneNeural stem cellsQuiescent neural stem cellsRostral migratory streamNew olfactory bulb neuronsNSC quiescenceOlfactory bulb neuronsLoss of Notch2Bulb neuronsNew neuronsAdult forebrainOB lineageAging-like phenotypesMigratory streamNotch2 functionNeuronsNotch2Canonical Notch signalingNeurogenesisStem cellsNotch signalingCell cycleForebrainQuiescence
2017
Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly
Sgourdou P, Mishra-Gorur K, Saotome I, Henagariu O, Tuysuz B, Campos C, Ishigame K, Giannikou K, Quon JL, Sestan N, Caglayan AO, Gunel M, Louvi A. Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly. Scientific Reports 2017, 7: 43708. PMID: 28272472, PMCID: PMC5341122, DOI: 10.1038/srep43708.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAurora Kinase BBrainCell CycleCell Cycle ProteinsCell DifferentiationCell ProliferationCentrosomeConsanguinityDisease Models, AnimalEpistasis, GeneticFluorescent Antibody TechniqueGene ExpressionHumansInheritance PatternsMaleMiceMice, KnockoutMicrocephalyMutationNerve Tissue ProteinsNeural Stem CellsPedigreeWhole Genome SequencingConceptsChromosome passenger complexPatient-derived fibroblastsCentrosome inheritanceNeocortical progenitorsDisease-associated mutant formsSpindle pole localizationAurora kinase BPassenger complexMitotic progressionMouse orthologDiverse functionsMutant formsWD repeat domain 62Key regulatorCPC componentsKinase BPole localizationPrimary microcephalyLate neurogenesisRecessive mutationsNeuronal differentiationWDR62Severe brain malformationsReduced proliferationNeocortical development
2016
Notch1 and Notch2 receptors regulate mouse and human gastric antral epithelial cell homoeostasis
Gifford GB, Demitrack ES, Keeley TM, Tam A, La Cunza N, Dedhia PH, Spence JR, Simeone DM, Saotome I, Louvi A, Siebel CW, Samuelson LC. Notch1 and Notch2 receptors regulate mouse and human gastric antral epithelial cell homoeostasis. Gut 2016, 66: 1001. PMID: 26933171, PMCID: PMC5009003, DOI: 10.1136/gutjnl-2015-310811.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, Monoclonal, HumanizedApoptosisCell DifferentiationCell ProliferationCells, CulturedDibenzazepinesEpithelial CellsFemaleGastric MucosaGene ExpressionHomeostasisHumansMaleMiceMice, Inbred C57BLMice, TransgenicOrganoidsPyloric AntrumReceptor, Notch1Receptor, Notch2Receptors, G-Protein-CoupledSignal TransductionStem CellsConceptsEpithelial cell homeostasisCell homeostasisNotch receptorsNotch inhibitor dibenzazepineGlobal Notch inhibitionStem cellsAntral stem cellsHuman antral glandsAnalysis of miceNotch pathway receptorsLgr5 stem cellsCellular differentiationNotch signalingNotch2 receptorMolecular approachesPathway receptorsNotch pathway inhibitionHuman organoidsEpithelial cell proliferationNotch inhibitionInhibition of Notch1Notch inhibitorsOrganoid growthCell proliferationNotch2
2011
Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock-In Mice
Fre S, Hannezo E, Sale S, Huyghe M, Lafkas D, Kissel H, Louvi A, Greve J, Louvard D, Artavanis-Tsakonas S. Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock-In Mice. PLOS ONE 2011, 6: e25785. PMID: 21991352, PMCID: PMC3185035, DOI: 10.1371/journal.pone.0025785.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsCell DifferentiationCell LineageClone CellsEnterocytesGene Knock-In TechniquesGene TargetingHomeodomain ProteinsIntegrasesIntestinesKineticsMiceMice, Inbred C57BLMice, TransgenicMicrovilliMultipotent Stem CellsReceptors, NotchSequence Homology, Amino AcidSignal TransductionStem CellsTranscription Factor HES-1Transcription, Genetic
2007
The derivatives of the Wnt3a lineage in the central nervous system
Louvi A, Yoshida M, Grove EA. The derivatives of the Wnt3a lineage in the central nervous system. The Journal Of Comparative Neurology 2007, 504: 550-569. PMID: 17701978, DOI: 10.1002/cne.21461.Peer-Reviewed Original ResearchConceptsSpinal cordCervical spinal cordTrigeminal sensory systemRostral spinal cordCentral nervous systemCentral auditory systemDorsal midlineGenetic fate mappingSpecific functional networksAdult brainNervous systemDorsal halfBrain structuresCordLineage cellsAuditory systemMidbrainCell fate specificationVertebrate neural tubeNeural tubeFate mappingFunctional networksTransient gene expressionMidlineFate specification
2004
Presenilin 1 in migration and morphogenesis in the central nervous system
Louvi A, Sisodia SS, Grove EA. Presenilin 1 in migration and morphogenesis in the central nervous system. Development 2004, 131: 3093-3105. PMID: 15163631, DOI: 10.1242/dev.01191.Peer-Reviewed Original ResearchMeSH KeywordsAmyloid Precursor Protein SecretasesAnimalsAspartic Acid EndopeptidasesBrainBrain StemBromodeoxyuridineCell DifferentiationCell DivisionCell MovementCentral Nervous SystemCerebellumColoring AgentsCyclin-Dependent Kinase 5Cyclin-Dependent KinasesCytoskeletonDopamine AgentsEndopeptidasesGene Expression Regulation, DevelopmentalHomozygoteImmunohistochemistryIn Situ HybridizationLightMembrane ProteinsMiceMutationNeuronsPresenilin-1Time FactorsConceptsCentral nervous systemNervous systemPresenilin 1Premature neuronal differentiationCNS morphogenesisCerebral cortexCortical dysplasiaCortical laminationExternal granule layerPontine nucleiPresenilin-1 functionCerebellar granule cell precursorsFacial branchiomotor nucleusTangential migratory pathwayCaudal midbrainGranule cell precursorsNeuronal cellsBrain developmentNeuronal migrationTangential migrationBranchiomotor nucleiCell precursorsNeuronal differentiationGranule layerMidline fusion