2024
Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions
Tanaka K, Chen M, Prendergast A, Zhuang Z, Nasiri A, Joshi D, Hintzen J, Chung M, Kumar A, Mani A, Koleske A, Crawford J, Nicoli S, Schwartz M. Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions. The EMBO Journal 2024, 43: 3175-3191. PMID: 38886581, PMCID: PMC11294477, DOI: 10.1038/s44318-024-00142-0.Peer-Reviewed Original ResearchLatrophilin-2Affinity purification methodCell-cell junctionsHuman genetic dataPECAM-1SiRNA screenGenetic dataEndothelial cell response to fluid shear stressGA proteinsDownstream eventsEndothelial-specific knockoutG-proteinActivity assayShear stress mechanotransductionPlexin-D1Endothelial signalingJunctional complexesPurification methodVE-cadherinResponse to fluid shear stressVascular developmentGA residuesEndothelial junctionsGPCRsVEGF receptors
2022
Author Correction: Syndecan-2 selectively regulates VEGF-induced vascular permeability
Corti F, Ristori E, Rivera-Molina F, Toomre D, Zhang J, Mihailovic J, Zhuang Z, Simons M. Author Correction: Syndecan-2 selectively regulates VEGF-induced vascular permeability. Nature Cardiovascular Research 2022, 1: 592-592. PMID: 39195875, DOI: 10.1038/s44161-022-00092-y.Peer-Reviewed Original Research
2015
Elevated follicular helper T Cells and expression of IL-21 in thyroid tissues are involved in the pathogenesis of Graves’ disease
Zhang J, Ren M, Zeng H, Guo Y, Zhuang Z, Feng Z, Yan H, Xu M, Liang W, Yang C, Cheng H, Ding H, Yan L. Elevated follicular helper T Cells and expression of IL-21 in thyroid tissues are involved in the pathogenesis of Graves’ disease. Immunologic Research 2015, 62: 163-174. PMID: 25894310, DOI: 10.1007/s12026-015-8647-z.Peer-Reviewed Original ResearchConceptsGD thyroid tissuesExpression of TfhFollicular helper T cellsThyroid follicular cellsPresence of TFHIL-21Helper T cellsThyroid tissueTSAb stimulationThyroid autoantibodiesIL-21RT cellsCAMP productionIL-21 stimulationPathogenesis of GDPathogenesis of GravesRelated factorsReal-time polymerase chain reactionNormal thyroid follicular cellsNormal thyroid tissueSerum levelsAutoimmune diseasesConfocal microscopyPolymerase chain reactionTfh
2013
Endothelial Cell–Dependent Regulation of Arteriogenesis
Moraes F, Paye J, Gabhann F, Zhuang ZW, Zhang J, Lanahan AA, Simons M. Endothelial Cell–Dependent Regulation of Arteriogenesis. Circulation Research 2013, 113: 1076-1086. PMID: 23897694, PMCID: PMC3865810, DOI: 10.1161/circresaha.113.301340.Peer-Reviewed Original ResearchConceptsAdult arteriogenesisCell-autonomous fashionGrowth factor signalingMouse linesCell-autonomous effectsKnockin mouse lineMorphogenetic defectsArterial morphogenesisCell type-specific deletionFactor signalingCell typesCre-driver mouse linesSynectinAttractive therapeutic strategyOcclusive atherosclerotic diseaseMuscle cellsEndothelial cellsRegulationArterial conduitsAtherosclerotic diseaseTherapeutic strategiesAdult miceClinical importanceArteriogenesisCellsNO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth
Jaba IM, Zhuang ZW, Li N, Jiang Y, Martin KA, Sinusas AJ, Papademetris X, Simons M, Sessa WC, Young LH, Tirziu D. NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth. Journal Of Clinical Investigation 2013, 123: 1718-1731. PMID: 23454748, PMCID: PMC3613910, DOI: 10.1172/jci65112.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, BiologicalAnimalsCell EnlargementCells, CulturedCoronary VesselsEndothelium, VascularHeart VentriclesMechanistic Target of Rapamycin Complex 1MiceMice, Inbred C57BLMice, TransgenicMultiprotein ComplexesMyocytes, CardiacNeovascularization, PhysiologicNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePlacenta Growth FactorPregnancy ProteinsProteinsProteolysisProto-Oncogene Proteins c-aktRatsRats, Sprague-DawleyRGS ProteinsSignal TransductionTOR Serine-Threonine KinasesConceptsCardiomyocyte growthAkt/mTORC1 signalingNovel NO-dependent mechanismProteasomal degradationCoordination of angiogenesisMTORC1 signalingConditional overexpressionMurine cardiac tissueG proteinsTransgenic expressionAkt/Physiological mechanismsMyocyte growthVessel growthGrowth factorTransgenic miceHypertrophic responseAngiogenesisKnockout miceMyocardial hypertrophyExpressionGrowthCardiac hypertrophyNOS inhibitor L-NAMEInduction
2012
Vascular adaptation to a dysfunctional endothelium as a consequence of Shb deficiency
Christoffersson G, Zang G, Zhuang ZW, Vågesjö E, Simons M, Phillipson M, Welsh M. Vascular adaptation to a dysfunctional endothelium as a consequence of Shb deficiency. Angiogenesis 2012, 15: 469-480. PMID: 22562363, PMCID: PMC4059510, DOI: 10.1007/s10456-012-9275-z.Peer-Reviewed Original ResearchConceptsShb knockout mouseWild-type situationShb adapter proteinPatho-physiological responsesKnockout miceAdapter proteinAdherens junctionsShb deficiencyFunction of VEGFVE-cadherinVivo angiogenesisPhysiological conditionsAltered propertiesReceptor VEGFR-2Growth factorVascular endothelial growth factorVEGFR-2
2011
Macrophage skewing by Phd2 haplodeficiency prevents ischaemia by inducing arteriogenesis
Takeda Y, Costa S, Delamarre E, Roncal C, Leite de Oliveira R, Squadrito ML, Finisguerra V, Deschoemaeker S, Bruyère F, Wenes M, Hamm A, Serneels J, Magat J, Bhattacharyya T, Anisimov A, Jordan BF, Alitalo K, Maxwell P, Gallez B, Zhuang ZW, Saito Y, Simons M, De Palma M, Mazzone M. Macrophage skewing by Phd2 haplodeficiency prevents ischaemia by inducing arteriogenesis. Nature 2011, 479: 122-126. PMID: 21983962, PMCID: PMC4659699, DOI: 10.1038/nature10507.Peer-Reviewed Original ResearchDevelopment and Application of a Multimodal Contrast Agent for SPECT/CT Hybrid Imaging
Criscione JM, Dobrucki LW, Zhuang ZW, Papademetris X, Simons M, Sinusas AJ, Fahmy TM. Development and Application of a Multimodal Contrast Agent for SPECT/CT Hybrid Imaging. Bioconjugate Chemistry 2011, 22: 1784-1792. PMID: 21851119, PMCID: PMC3204385, DOI: 10.1021/bc200162r.Peer-Reviewed Original ResearchFGF-dependent regulation of VEGF receptor 2 expression in mice
Murakami M, Nguyen LT, Hatanaka K, Schachterle W, Chen PY, Zhuang ZW, Black BL, Simons M. FGF-dependent regulation of VEGF receptor 2 expression in mice. Journal Of Clinical Investigation 2011, 121: 2668-2678. PMID: 21633168, PMCID: PMC3223828, DOI: 10.1172/jci44762.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedEndothelial CellsEnzyme ActivationFibroblast Growth FactorsHindlimbHumansIschemiaMiceMice, Inbred C57BLMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Neovascularization, PhysiologicProto-Oncogene Proteins c-etsSignal TransductionVascular Endothelial Growth Factor Receptor-2Vascular Endothelial Growth FactorsConceptsFGF-dependent regulationEts transcription factorsERK1/2-dependent mannerVEGF receptor-2 expressionActivation of ERK1/2Enhancer activationTranscriptional analysisTranscription factorsVEGFR2 expressionVascular morphogenesisFGF stimulationVEGF stimulationFGFVascular formationExpressionVascular integrityReceptor 2 expressionActivationMorphogenesisVEGFERK1/2Numerous studiesPathwayRegulationDownregulationChallenging the Surgical Rodent Hindlimb Ischemia Model with the Miniinterventional Technique
Zhuang ZW, Shi J, Rhodes JM, Tsapakos MJ, Simons M. Challenging the Surgical Rodent Hindlimb Ischemia Model with the Miniinterventional Technique. Journal Of Vascular And Interventional Radiology 2011, 22: 1437-1446. PMID: 21459613, PMCID: PMC3367666, DOI: 10.1016/j.jvir.2010.12.039.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Flow VelocityBone Marrow TransplantationChemokinesCytokinesDisease Models, AnimalEmbolization, TherapeuticFemoral ArteryHindlimbInflammation MediatorsIschemiaLaser-Doppler FlowmetryLigationMaleMuscle, SkeletalNeovascularization, PhysiologicRatsRats, Inbred LewRecovery of FunctionRegional Blood FlowTime FactorsConceptsMarrow mononuclear cellsLaser Doppler imagingRight femoral arterySurgical ligationFemoral arteryHindlimb ischemia modelSL groupInterventional embolizationIschemia modelBone marrow mononuclear cellsGranulocyte colony-stimulating factorPreserves tissue integrityColony-stimulating factorVivo microscopic imagesHindlimb ischemic modelChemokine assaysMassive scarringInflammatory infiltrationIL-18Lewis ratsNerve degenerationIL-13IL-6Mononuclear cellsIschemic model
2010
Tissue-Engineered Lungs for in Vivo Implantation
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.Peer-Reviewed Original ResearchConceptsLung tissueLung matrixAcellular lung matrixNative lung tissueTissue-engineered lungsLung transplantationPrimary therapyAdult lung tissueAdult ratsPulmonary epitheliumVascular endotheliumFunctional lungLung regenerationVascular compartmentLungSeeded endothelial cellsMechanical characteristicsEndothelial cellsVivo implantationRatsEpitheliumTissueCellular componentsExtracellular matrixGas exchangeVEGF Receptor 2 Endocytic Trafficking Regulates Arterial Morphogenesis
Lanahan AA, Hermans K, Claes F, Kerley-Hamilton JS, Zhuang ZW, Giordano FJ, Carmeliet P, Simons M. VEGF Receptor 2 Endocytic Trafficking Regulates Arterial Morphogenesis. Developmental Cell 2010, 18: 713-724. PMID: 20434959, PMCID: PMC2875289, DOI: 10.1016/j.devcel.2010.02.016.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsArteriesCarrier ProteinsCell MembraneEndocytosisEndothelium, VascularGene SilencingMiceMice, KnockoutMorphogenesisMyosin Heavy ChainsNeuropeptidesPhosphorylationVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ZebrafishConceptsArterial morphogenesisSelective dephosphorylationPlasma membraneIntracellular traffickingKey growth factorsNull phenotypeMyosin VIMolecular eventsMorphogenesisVEGFR2 activationTraffickingIntracellular sitesKey eventsPTP1BNull miceKnockdownImpaired VEGFGrowth factorVEGFR2ActivationDephosphorylationEndosomesVEGFPhosphorylationSitesAging‐induced collateral impairment: role of arterial rarefaction, decreased eNOS expression/signaling, and increased susceptibility of endothelial cells to apoptosis
Epstein S, Wang J, Peng X, Zhuang Z, Simons M, Xue Z, Burnett M. Aging‐induced collateral impairment: role of arterial rarefaction, decreased eNOS expression/signaling, and increased susceptibility of endothelial cells to apoptosis. The FASEB Journal 2010, 24: 294.3-294.3. DOI: 10.1096/fasebj.24.1_supplement.294.3.Peer-Reviewed Original ResearchWild-type miceEndothelial cellsOld miceEndothelial nitric oxide synthase proteinNitric oxide synthase proteinOld wild-type micePositive animal studiesYoung wild-type miceENOS-KO miceENOS knockout miceDisappointing clinical resultsPre-existing arteriolesVessel phenotypeENOS expressionENOS proteinKO miceC57 miceClinical resultsYoung miceArteriolar rarefactionAge-induced changesKnockout miceAnimal studiesTherapeutic interventionsAngiogenesis therapyERK1/2-Akt1 crosstalk regulates arteriogenesis in mice and zebrafish
Ren B, Deng Y, Mukhopadhyay A, Lanahan AA, Zhuang ZW, Moodie KL, Mulligan-Kehoe MJ, Byzova TV, Peterson RT, Simons M. ERK1/2-Akt1 crosstalk regulates arteriogenesis in mice and zebrafish. Journal Of Clinical Investigation 2010, 120: 1217-1228. PMID: 20237411, PMCID: PMC2846043, DOI: 10.1172/jci39837.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsArteriesCarrier ProteinsMaleMiceMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorphogenesisNeovascularization, PhysiologicNeuropeptidesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ZebrafishZebrafish Proteins
2008
FGF mediates maintenance of vascular integrity in a Csk dependent manner
Murakami M, Zhuang Z, Moodie K, Stan R, Simons M. FGF mediates maintenance of vascular integrity in a Csk dependent manner. The FASEB Journal 2008, 22: 329.5-329.5. DOI: 10.1096/fasebj.22.1_supplement.329.5.Peer-Reviewed Original ResearchInhibition of FGFVE-cadherinVE-cadherin mutantCo-immunoprecipitation experimentsDominant-negative formCell-cell contactVE-cadherin complexVE-cadherin internalizationVE-cadherin stabilizationTotal expression levelsVascular integrityCSK associationFGF signalingP120-cateninEC monolayer permeabilitySrc activationChimera proteinTransgenic expressionFGF inhibitionFGFAdult vasculatureExpression levelsFGF-R1ProteinTie2 promoter
2006
Arteriogenesis: Noninvasive Quantification with Multi–Detector Row CT Angiography and Three-dimensional Volume Rendering in Rodents
Zhuang ZW, Gao L, Murakami M, Pearlman JD, Sackett TJ, Simons M, de Muinck ED. Arteriogenesis: Noninvasive Quantification with Multi–Detector Row CT Angiography and Three-dimensional Volume Rendering in Rodents. Radiology 2006, 240: 698-707. PMID: 16926325, DOI: 10.1148/radiol.2403050976.Peer-Reviewed Original ResearchConceptsBone marrow-derived mononuclear cellsTotal arterial areaBlood vessel areaCT angiographyNonischemic legsBMC groupArterial areaMarrow-derived mononuclear cellsTotal blood vessel areaMulti-detector row CT angiographyVessel areaRight femoral arteryUnpaired Student's t-testTotal blood vessel volumeBlood vessel densityMulti-detector rowArterial growthBlood vessel volumeStudent's t-testTwo-way analysisRow CT angiographyBMC injectionBarium sulfate suspensionLewis ratsMore arteries
2005
An interactive 3D visualization and manipulation tool for effective assessment of angiogenesis and arteriogenesis using computed tomographic angiography
Shen L, Gao L, Zhuang Z, DeMuinck E, Huang H, Makedon F, Pearlman J. An interactive 3D visualization and manipulation tool for effective assessment of angiogenesis and arteriogenesis using computed tomographic angiography. Proceedings Of SPIE--the International Society For Optical Engineering 2005, 5744: 848-858. DOI: 10.1117/12.596138.Peer-Reviewed Original Research
2004
1121-52 Inhibition of in-stent restenosis in porcine coronary arteries by copper chelation
Mandinov L, Moodie K, Mandinova A, Zhuang Z, Maciag T, Simons M, de Muinck E. 1121-52 Inhibition of in-stent restenosis in porcine coronary arteries by copper chelation. Journal Of The American College Of Cardiology 2004, 43: a78. DOI: 10.1016/s0735-1097(04)90323-3.Peer-Reviewed Original ResearchAdventitial Angiogenesis Drives Neointima Formation
Khurana R, Martin J, Zachary I, Zhuang Z, De Muinck E, Simons M, Bhardwaj S, Yla-Herttuala S, Ferrara N. Adventitial Angiogenesis Drives Neointima Formation. Clinical Science 2004, 106: 1p-1p. DOI: 10.1042/cs106001p.Peer-Reviewed Original Research