2010
Construction and maintenance of randomized retroviral expression libraries for transmembrane protein engineering
Marlatt SA, Kong Y, Cammett TJ, Korbel G, Noonan JP, DiMaio D. Construction and maintenance of randomized retroviral expression libraries for transmembrane protein engineering. Protein Engineering Design And Selection 2010, 24: 311-320. PMID: 21149273, PMCID: PMC3038463, DOI: 10.1093/protein/gzq112.Peer-Reviewed Original ResearchConceptsSignificant genetic bottleneckRetroviral expression libraryDeep DNA sequencingAmino acid segmentNovel biological activitiesGenetic bottleneckTransmembrane domainMammalian cellsLibrary sequencesRandom mutagenesisProtein engineeringExpression libraryDeep sequencingDifferent proteinsSmall proteinsGenetic selectionAcid segmentRetroviral libraryDNA sequencingSequencing resultsRandomized libraryProteinPowerful approachSequencingDiversity
2003
A Protein Interaction Map of Drosophila melanogaster
Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, Vijayadamodar G, Pochart P, Machineni H, Welsh M, Kong Y, Zerhusen B, Malcolm R, Varrone Z, Collis A, Minto M, Burgess S, McDaniel L, Stimpson E, Spriggs F, Williams J, Neurath K, Ioime N, Agee M, Voss E, Furtak K, Renzulli R, Aanensen N, Carrolla S, Bickelhaupt E, Lazovatsky Y, DaSilva A, Zhong J, Stanyon CA, Finley RL, White KP, Braverman M, Jarvie T, Gold S, Leach M, Knight J, Shimkets RA, McKenna MP, Chant J, Rothberg JM. A Protein Interaction Map of Drosophila melanogaster. Science 2003, 302: 1727-1736. PMID: 14605208, DOI: 10.1126/science.1090289.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCell CycleCell DifferentiationCloning, MolecularComputational BiologyDNA, ComplementaryDrosophila melanogasterDrosophila ProteinsErbB ReceptorsGenes, InsectImmunity, InnateMathematicsModels, StatisticalPhotoreceptor Cells, InvertebrateProtein BindingProtein Interaction MappingProteomeRNA SplicingRNA, MessengerSignal TransductionTranscription, GeneticTwo-Hybrid System TechniquesConceptsProtein interaction mapsDrosophila melanogasterDraft mapComplementary DNA librarySystems biology modelingHigh-confidence mapMulticellular organismsFly proteomeMultiprotein complexesDNA libraryInteraction mapPathway componentsLevels of organizationKnown pathwaysExtended pathwaysHuman biologyMelanogasterModel systemProteinPathwayProteomeComputational methodsTranscriptsOrganismsBiology