2013
Forward genetics identifies Kdf1/1810019J16Rik as an essential regulator of the proliferation–differentiation decision in epidermal progenitor cells
Lee S, Kong Y, Weatherbee SD. Forward genetics identifies Kdf1/1810019J16Rik as an essential regulator of the proliferation–differentiation decision in epidermal progenitor cells. Developmental Biology 2013, 383: 201-213. PMID: 24075906, PMCID: PMC3841015, DOI: 10.1016/j.ydbio.2013.09.022.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAllelesAnimalsBase SequenceCell DifferentiationCell ProliferationCleft PalateEmbryo LossEpidermisFemaleGene Expression Regulation, DevelopmentalGenes, RecessiveGenetic Complementation TestHeterozygoteKeratinocytesMaleMiceMice, Inbred C57BLMolecular Sequence DataPhenotypePhosphoproteinsPoint MutationProtein BindingProteinsRNA SplicingStem CellsTrans-ActivatorsConceptsEpidermal progenitor cellsEssential regulatorProgenitor cellsProliferation-differentiation balanceUnbiased genetic screenCell fate decisionsProliferation-differentiation decisionsCell proliferationUncontrolled cell proliferationFactor 1 geneProgenitor cell proliferationGenetic screenFate decisionsRepressive signalsTerminal fateInductive signalsPremature differentiationGenetic mechanismsInhibition of p63Terminal differentiationCell differentiationEpidermal cellsKDF1Rapid turnoverRate of proliferation
2012
Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencing
del Viso F, Bhattacharya D, Kong Y, Gilchrist MJ, Khokha MK. Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencing. BMC Genomics 2012, 13: 649. PMID: 23171430, PMCID: PMC3526394, DOI: 10.1186/1471-2164-13-649.Peer-Reviewed Original ResearchConceptsBulk segregant analysisForward genetic screenSegregant analysisGenetic screenGenome assemblyExon captureCausative mutationsVertebrate model systemHigh-throughput sequencingHuman genetic analysisThousands of SNPsAssembly of scaffoldsModel systemGenomic resourcesVertebrate modelXenopus tropicalisFine mappingGenetic analysisCapture sequencingSequence variantsSequencingMutationsRapid discoveryMutantsExome sequencing