2001
Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe
Henkel J, Du H, Yang P, Qyang Y, Kansra S, Ko M, Kim H, Marcus S. Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe. Differentiation 2001, 67: 98-106. PMID: 11683500, DOI: 10.1046/j.1432-0436.2001.670402.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCatalysisCell DifferentiationConserved SequenceCytoskeletonHumansMicroscopy, FluorescenceMolecular Sequence DataMutationProtein BindingProtein KinasesRepressor ProteinsReproduction, AsexualSchizosaccharomycesSchizosaccharomyces pombe ProteinsSubstrate SpecificityThiabendazoleTwo-Hybrid System TechniquesConceptsS. pombe cellsFission yeastPombe cellsObvious growth defectTwo-hybrid screenRegulators of actinSexual differentiationInhibition of matingMating deficiencyEukaryotic organismsMAPK moduleNull mutantsS. pombeCytoskeletal controlGrowth defectCytoskeletal defectsMating responseByr1Diverse rolesMutantsMolecular characterizationYeastProteinSchizosaccharomycesOverexpression
1999
Cell-Type-Dependent Activity of the Ubiquitous Transcription Factor USF in Cellular Proliferation and Transcriptional Activation
Qyang Y, Luo X, Lu T, Ismail P, Krylov D, Vinson C, Sawadogo M. Cell-Type-Dependent Activity of the Ubiquitous Transcription Factor USF in Cellular Proliferation and Transcriptional Activation. Molecular And Cellular Biology 1999, 19: 1508-1517. PMID: 9891084, PMCID: PMC116079, DOI: 10.1128/mcb.19.2.1508.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell DivisionCell LineDNADNA-Binding ProteinsGene ExpressionGenes, ReporterHeLa CellsHumansImmediate-Early ProteinsModels, BiologicalMolecular Sequence DataRecombinant Fusion ProteinsSubcellular FractionsTrans-ActivatorsTranscription FactorsTranscriptional ActivationTransfectionUpstream Stimulatory FactorsViral Envelope ProteinsConceptsUSF-specific regionDNA-binding activityUSF proteinsSaos-2 cellsTranscriptional activationAutonomous transcriptional activation domainsTranscriptional activityTranscriptional activation domainTranscription factor USFActivity of USFHelix transcription factorCellular proliferationCell linesActivation domainUSF activityUSF functionSaos-2 osteosarcoma cell lineSubcellular localizationTranscription factorsInitiator elementReporter geneMutational analysisTransient cotransfectionFusion proteinOsteosarcoma cell lines