Methods of Cell Purification: A Critical Juncture for Laboratory Research and Translational Science
Amos PJ, Bozkulak E, Qyang Y. Methods of Cell Purification: A Critical Juncture for Laboratory Research and Translational Science. Cells Tissues Organs 2011, 195: 26-40. PMID: 21996576, PMCID: PMC3257814, DOI: 10.1159/000331390.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell SeparationFlow CytometryHumansRecombination, GeneticTranslational Research, BiomedicalVirusesConceptsCell purificationSpecific cell populationsPhenotypic traitsCell biologySurface proteinsGenetic expressionCellular reagentsSpecific cellsCell populationsSorting cellsCell separation strategiesPurificationCellsHeterogeneous mixtureAbility of researchersBiologyTraitsProteinCertain mechanismsExpressionDerivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells
Lee MY, Sun B, Schliffke S, Yue Z, Ye M, Paavola J, Bozkulak EC, Amos PJ, Ren Y, Ju R, Jung YW, Ge X, Yue L, Ehrlich BE, Qyang Y. Derivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells. Stem Cell Research 2011, 8: 49-57. PMID: 22099020, PMCID: PMC3222859, DOI: 10.1016/j.scr.2011.08.004.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdherens JunctionsAnimalsBacterial ProteinsBase SequenceCadherinsCalciumCell Culture TechniquesCell LineCell SeparationConnexin 43Embryonic Stem CellsFlow CytometryGap JunctionsHeart VentriclesImaging, Three-DimensionalIntegrasesLuminescent ProteinsMiceMyocytes, CardiacMyosin Light ChainsPromoter Regions, GeneticProteinsRNA, UntranslatedConceptsVentricular cardiomyocytesCalcium transientsNeonatal mouse ventricular cardiomyocytesFunctional excitation-contraction couplingCardiac contractile performanceDouble transgenic miceCurrent-clamp recordingsIntracellular calcium transientsExcitation-contraction couplingAction potential characteristicsMouse ventricular cardiomyocytesMyosin light chain 2vFluorescence-activated cell sortingAdrenergic signalingIntracellular calciumContractile performanceClamp recordingsTransgenic miceElectrical stimulationCardiac repairInduction of differentiationIsoproterenol stimulationExpression of YFPMouse linesN-cadherin