2011
Derivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells
Lee MY, Sun B, Schliffke S, Yue Z, Ye M, Paavola J, Bozkulak EC, Amos PJ, Ren Y, Ju R, Jung YW, Ge X, Yue L, Ehrlich BE, Qyang Y. Derivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells. Stem Cell Research 2011, 8: 49-57. PMID: 22099020, PMCID: PMC3222859, DOI: 10.1016/j.scr.2011.08.004.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdherens JunctionsAnimalsBacterial ProteinsBase SequenceCadherinsCalciumCell Culture TechniquesCell LineCell SeparationConnexin 43Embryonic Stem CellsFlow CytometryGap JunctionsHeart VentriclesImaging, Three-DimensionalIntegrasesLuminescent ProteinsMiceMyocytes, CardiacMyosin Light ChainsPromoter Regions, GeneticProteinsRNA, UntranslatedConceptsVentricular cardiomyocytesCalcium transientsNeonatal mouse ventricular cardiomyocytesFunctional excitation-contraction couplingCardiac contractile performanceDouble transgenic miceCurrent-clamp recordingsIntracellular calcium transientsExcitation-contraction couplingAction potential characteristicsMouse ventricular cardiomyocytesMyosin light chain 2vFluorescence-activated cell sortingAdrenergic signalingIntracellular calciumContractile performanceClamp recordingsTransgenic miceElectrical stimulationCardiac repairInduction of differentiationIsoproterenol stimulationExpression of YFPMouse linesN-cadherin
2001
Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe
Henkel J, Du H, Yang P, Qyang Y, Kansra S, Ko M, Kim H, Marcus S. Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe. Differentiation 2001, 67: 98-106. PMID: 11683500, DOI: 10.1046/j.1432-0436.2001.670402.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCatalysisCell DifferentiationConserved SequenceCytoskeletonHumansMicroscopy, FluorescenceMolecular Sequence DataMutationProtein BindingProtein KinasesRepressor ProteinsReproduction, AsexualSchizosaccharomycesSchizosaccharomyces pombe ProteinsSubstrate SpecificityThiabendazoleTwo-Hybrid System TechniquesConceptsS. pombe cellsFission yeastPombe cellsObvious growth defectTwo-hybrid screenRegulators of actinSexual differentiationInhibition of matingMating deficiencyEukaryotic organismsMAPK moduleNull mutantsS. pombeCytoskeletal controlGrowth defectCytoskeletal defectsMating responseByr1Diverse rolesMutantsMolecular characterizationYeastProteinSchizosaccharomycesOverexpression