Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe
Henkel J, Du H, Yang P, Qyang Y, Kansra S, Ko M, Kim H, Marcus S. Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe. Differentiation 2001, 67: 98-106. PMID: 11683500, DOI: 10.1046/j.1432-0436.2001.670402.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCatalysisCell DifferentiationConserved SequenceCytoskeletonHumansMicroscopy, FluorescenceMolecular Sequence DataMutationProtein BindingProtein KinasesRepressor ProteinsReproduction, AsexualSchizosaccharomycesSchizosaccharomyces pombe ProteinsSubstrate SpecificityThiabendazoleTwo-Hybrid System TechniquesConceptsS. pombe cellsFission yeastPombe cellsObvious growth defectTwo-hybrid screenRegulators of actinSexual differentiationInhibition of matingMating deficiencyEukaryotic organismsMAPK moduleNull mutantsS. pombeCytoskeletal controlGrowth defectCytoskeletal defectsMating responseByr1Diverse rolesMutantsMolecular characterizationYeastProteinSchizosaccharomycesOverexpressionGenetic and Molecular Characterization of Skb15, a Highly Conserved Inhibitor of the Fission Yeast PAK, Shk1
Kim H, Yang P, Qyang Y, Lai H, Du H, Henkel J, Kumar K, Bao S, Liu M, Marcus S. Genetic and Molecular Characterization of Skb15, a Highly Conserved Inhibitor of the Fission Yeast PAK, Shk1. Molecular Cell 2001, 7: 1095-1101. PMID: 11389855, DOI: 10.1016/s1097-2765(01)00248-9.Peer-Reviewed Original ResearchConceptsFission yeastP21-activated kinaseCytokinetic functionCytokinetic machineryCell polarityProtein functionSkb15Cytoskeletal remodelingMouse homologMicrotubule biogenesisShk1Mating responseNegative regulationActin polymerizationNull mutationCell cycleMolecular characterizationYeastSubstantial uncouplingProper executionPartial lossSchizosaccharomycesBiogenesisHomologKinase