2002
The p21‐activated kinase, Shk1, is required for proper regulation of microtubule dynamics in the fission yeast, Schizosaccharomyces pombe
Qyang Y, Yang P, Du H, Lai H, Kim H, Marcus S. The p21‐activated kinase, Shk1, is required for proper regulation of microtubule dynamics in the fission yeast, Schizosaccharomyces pombe. Molecular Microbiology 2002, 44: 325-334. PMID: 11972773, DOI: 10.1046/j.1365-2958.2002.02882.x.Peer-Reviewed Original ResearchConceptsFission yeastP21-activated kinaseMicrotubule dynamicsMicrotubule spindleProper regulationCell endsFission yeast cellsMicrotubule-destabilizing drug thiabendazoleGreen fluorescent proteinShk1 functionCell polarityKinase functionMutant cellsSpindle defectsShk1Microtubule defectsCold sensitiveDrug thiabendazoleKinase activityInterphase microtubulesFusion proteinYeast cellsPotential regulatorFluorescent proteinRelease of cells
1999
Cell-Type-Dependent Activity of the Ubiquitous Transcription Factor USF in Cellular Proliferation and Transcriptional Activation
Qyang Y, Luo X, Lu T, Ismail P, Krylov D, Vinson C, Sawadogo M. Cell-Type-Dependent Activity of the Ubiquitous Transcription Factor USF in Cellular Proliferation and Transcriptional Activation. Molecular And Cellular Biology 1999, 19: 1508-1517. PMID: 9891084, PMCID: PMC116079, DOI: 10.1128/mcb.19.2.1508.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell DivisionCell LineDNADNA-Binding ProteinsGene ExpressionGenes, ReporterHeLa CellsHumansImmediate-Early ProteinsModels, BiologicalMolecular Sequence DataRecombinant Fusion ProteinsSubcellular FractionsTrans-ActivatorsTranscription FactorsTranscriptional ActivationTransfectionUpstream Stimulatory FactorsViral Envelope ProteinsConceptsUSF-specific regionDNA-binding activityUSF proteinsSaos-2 cellsTranscriptional activationAutonomous transcriptional activation domainsTranscriptional activityTranscriptional activation domainTranscription factor USFActivity of USFHelix transcription factorCellular proliferationCell linesActivation domainUSF activityUSF functionSaos-2 osteosarcoma cell lineSubcellular localizationTranscription factorsInitiator elementReporter geneMutational analysisTransient cotransfectionFusion proteinOsteosarcoma cell lines