2024
Post-Translational Regulation of a Bidomain Glycerol-3-Phosphate Dehydrogenase Catalyzing Glycerol Synthesis under Salinity Stress in Chlamydomonas reinhardtii
Cruz-Powell I, Subedi B, Kim Y, Morales-Sánchez D, Cerutti H. Post-Translational Regulation of a Bidomain Glycerol-3-Phosphate Dehydrogenase Catalyzing Glycerol Synthesis under Salinity Stress in Chlamydomonas reinhardtii. Phycology 2024, 4: 213-234. DOI: 10.3390/phycology4020012.Peer-Reviewed Original ResearchGlycerol-3-phosphate dehydrogenaseGlycerol-3-phosphate phosphataseGlycerol-3-phosphateGlycerol synthesisWild typeInhibitor of cytoplasmic protein synthesisPlastid-localized enzymeResponse of microalgaePost-translational regulationTransgenic strainsCytoplasmic protein synthesisSynthesis of glycerolHigh salinityExposure to high salinityCore chlorophytesInhibitors of protein kinasesChlamydomonas reinhardtiiGlycerol accumulationNutrient deprivationGPD2Protein kinaseProtein activityAccumulate glycerolSalt stressEnvironmental stress
2018
Identification and functional study of the endoplasmic reticulum stress sensor IRE1 in Chlamydomonas reinhardtii
Yamaoka Y, Choi BY, Kim H, Shin S, Kim Y, Jang S, Song W, Cho CH, Yoon HS, Kohno K, Lee Y. Identification and functional study of the endoplasmic reticulum stress sensor IRE1 in Chlamydomonas reinhardtii. The Plant Journal 2018, 94: 91-104. PMID: 29385296, DOI: 10.1111/tpj.13844.Peer-Reviewed Original ResearchConceptsStress sensor IRE1Unfolded protein responseIre1 mutantsER stress responseMarker gene expressionGene expressionTandem zinc finger domainStress responseER stress sensor IRE1Cytosolic C-terminal regionEndoplasmic reticulum (ER) stress sensor IRE1Zinc finger domainER stress conditionsC-terminal regionN-terminal regionROS scavenger glutathioneER stress inducersStrain CCYeast Ire1pEvolutionary historyER homeostasisEndoplasmic reticulum stressChlamydomonas reinhardtiiProtein responseChimeric protein
2017
A multidomain enzyme, with glycerol‐3‐phosphate dehydrogenase and phosphatase activities, is involved in a chloroplastic pathway for glycerol synthesis in Chlamydomonas reinhardtii
Morales‐Sánchez D, Kim Y, Terng EL, Peterson L, Cerutti H. A multidomain enzyme, with glycerol‐3‐phosphate dehydrogenase and phosphatase activities, is involved in a chloroplastic pathway for glycerol synthesis in Chlamydomonas reinhardtii. The Plant Journal 2017, 90: 1079-1092. PMID: 28273364, DOI: 10.1111/tpj.13530.Peer-Reviewed Original ResearchConceptsMultidomain enzymeNitrogen deprivationPhosphatase activityGreen alga C.Dihydroxyacetone phosphateGlycerol synthesisGlycerol-3-phosphate dehydrogenaseHigh salinityCore chlorophytesPhosphatase motifPlastid pathwayChloroplastic pathwayAlgal speciesDehydrogenase domainChlamydomonas reinhardtiiChlamydomonas cellsMembrane glycerolipidsHyperosmotic stressCompatible solutesTAG accumulationBifunctional enzymeAlgal metabolismHypertonic stressFusion proteinTriacylglycerol accumulation