SECAT: Quantifying Protein Complex Dynamics across Cell States by Network-Centric Analysis of SEC-SWATH-MS Profiles
Rosenberger G, Heusel M, Bludau I, Collins BC, Martelli C, Williams EG, Xue P, Liu Y, Aebersold R, Califano A. SECAT: Quantifying Protein Complex Dynamics across Cell States by Network-Centric Analysis of SEC-SWATH-MS Profiles. Cell Systems 2020, 11: 589-607.e8. PMID: 33333029, PMCID: PMC8034988, DOI: 10.1016/j.cels.2020.11.006.Peer-Reviewed Original ResearchConceptsProtein-protein interactionsProtein complexesCell statesProtein complex dynamicsNative protein complexesMacromolecular complex formationPaper's transparent peer review processProtein interaction networksSEC-SWATHMultiple cell statesNetwork-centric analysisCellular processesInteraction networksMass spectrometric data analysisProteome OrganizationMolecular mechanismsRegulatory roleMass spectrometric analysisNetwork-based studyMultiplexed characterizationComplex formationSpectrometric data analysisSpectrometric analysisAlgorithmic toolkitState-specific changesA Global Screen for Assembly State Changes of the Mitotic Proteome by SEC-SWATH-MS
Heusel M, Frank M, Köhler M, Amon S, Frommelt F, Rosenberger G, Bludau I, Aulakh S, Linder MI, Liu Y, Collins BC, Gstaiger M, Kutay U, Aebersold R. A Global Screen for Assembly State Changes of the Mitotic Proteome by SEC-SWATH-MS. Cell Systems 2020, 10: 133-155.e6. PMID: 32027860, PMCID: PMC7042714, DOI: 10.1016/j.cels.2020.01.001.Peer-Reviewed Original ResearchConceptsSEC-SWATHHundreds of complexesThousands of proteinsAssembly state changesMitotic proteomeProtein complexesProteomic studiesGlobal screenHuman cellsComplex remodelingHigher-level organizationBiological researchKey hallmarksBiochemical reactionsInteractive exploration toolProteinFunctional stateSpecific changesComplexesProteomeCellsMitosisHallmarkRemodelingInterphase