2006
p204 Is Required for the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes ITS EXPRESSION IS ACTIVATED BY THE CARDIAC GATA4, NKX2.5, AND TBX5 PROTEINS*
Ding B, Liu CJ, Huang Y, Hickey RP, Yu J, Kong W, Lengyel P. p204 Is Required for the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes ITS EXPRESSION IS ACTIVATED BY THE CARDIAC GATA4, NKX2.5, AND TBX5 PROTEINS*. Journal Of Biological Chemistry 2006, 281: 14882-14892. PMID: 16556595, DOI: 10.1074/jbc.m511747200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCell Line, TumorChromatinGATA4 Transcription FactorGene Expression Regulation, DevelopmentalHomeobox Protein Nkx-2.5Homeodomain ProteinsMiceMolecular Sequence DataMuscle, SkeletalMyocytes, CardiacNuclear ProteinsPhosphoproteinsT-Box Domain ProteinsTranscription FactorsConceptsNuclear export signalExport signalTranscription factorsP19 cellsC2C12 skeletal muscle myoblastsP19 murine embryonal carcinoma cellsEmbryonal carcinoma stem cellsAdult mouse tissuesMurine embryonal carcinoma cellsTBX5 transcription factorSkeletal muscle myoblastsEmbryonal carcinoma cellsCarcinoma stem cellsP204 expressionP204 proteinExpression of GATA4Regulatory regionsTBX5 proteinReporter constructsAntisense RNAMuscle myoblastsCardiac myocytesMouse tissuesStem cellsGATA4An engineered VEGF‐activating zinc finger protein transcription factor improves blood flow and limb salvage in advanced‐age mice
Yu J, Lei L, Liang Y, Hinh L, Hickey RP, Huang Y, Liu D, Yeh JL, Rebar E, Case C, Spratt K, Sessa WC, Giordano FJ. An engineered VEGF‐activating zinc finger protein transcription factor improves blood flow and limb salvage in advanced‐age mice. The FASEB Journal 2006, 20: 479-481. PMID: 16423874, DOI: 10.1096/fj.04-3670fje.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAgingAmino Acid SequenceAnimalsBlood Flow VelocityFeasibility StudiesGene Expression RegulationGenes, SyntheticGenetic TherapyGenetic VectorsHindlimbIschemiaLaser-Doppler FlowmetryMiceMice, Inbred C57BLMolecular Sequence DataNeovascularization, PhysiologicProtein EngineeringRecombinant ProteinsRNA, MessengerStructure-Activity RelationshipTranscription FactorsVascular Endothelial Growth Factor AZinc FingersConceptsLimb salvageBlood flowHindlimb ischemiaC57/BL6 micePeripheral vascular diseaseVascular endothelial growth factorPotential clinical utilityEndothelial growth factorExpression of VEGFABL6 miceIschemic limbsVascular diseaseIschemic hindlimbMurine modelClinical utilityVessel countProtein transcription factorsGrowth factorProtein levelsSalvage
2002
Induction of angiogenesis in a mouse model using engineered transcription factors
Rebar EJ, Huang Y, Hickey R, Nath AK, Meoli D, Nath S, Chen B, Xu L, Liang Y, Jamieson AC, Zhang L, Spratt SK, Case CC, Wolffe A, Giordano FJ. Induction of angiogenesis in a mouse model using engineered transcription factors. Nature Medicine 2002, 8: 1427-1432. PMID: 12415262, DOI: 10.1038/nm1202-795.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAngiogenesis Inducing AgentsAnimalsDrug DesignGene Expression RegulationGenetic TherapyMiceModels, AnimalMolecular Sequence DataNeovascularization, PhysiologicProtein EngineeringRecombinant ProteinsTranscription FactorsVascular Endothelial Growth Factor AZinc FingersConceptsTranscription factorsEndogenous genesZinc finger protein transcription factorsProtein transcription factorsWhole-organism modelDNA sequencesInduced expressionGenesInduction of angiogenesisZFPExpression of VEGFAProtein VEGFExpressionGrowth factorStimulation of angiogenesisTissue cultureVascular endothelial growth factorExperimental wound healingEndothelial growth factorWound healingNatural arraysAngiogenesisVivoCDNAMouse model