2012
Visualization of Src and FAK Activity during the Differentiation Process from HMSCs to Osteoblasts
Liao X, Lu S, Zhuo Y, Winter C, Xu W, Wang Y. Visualization of Src and FAK Activity during the Differentiation Process from HMSCs to Osteoblasts. PLOS ONE 2012, 7: e42709. PMID: 22900044, PMCID: PMC3416797, DOI: 10.1371/journal.pone.0042709.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseCell DifferentiationCells, CulturedEnzyme ActivationFocal Adhesion Protein-Tyrosine KinasesHumansKineticsMesenchymal Stem CellsOsteoblastsOsteogenesisSpectrophotometry, UltravioletSrc-Family KinasesConceptsSrc activationFluorescence resonance energy transferFAK activationDifferentiation processActivation of FAKRegulates cellular adhesionSrc biosensorKinase FAKInduction of differentiationResonance energy transferFAKBiochemical signalsSrcCellular adhesionLiving cellsStem cellsOsteoblast differentiation processDifferentiationCellsBone cellsSrc/FAKInductionMesenchymal stem cellsActivityGenetics
2011
Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer
Seong J, Ouyang M, Kim T, Sun J, Wen P, Lu S, Zhuo Y, Llewellyn N, Schlaepfer D, Guan J, Chien S, Wang Y. Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Nature Communications 2011, 2: 406. PMID: 21792185, PMCID: PMC3373894, DOI: 10.1038/ncomms1414.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell MembraneEnzyme ActivationFluorescence Resonance Energy TransferFocal Adhesion Protein-Tyrosine KinasesMiceMice, KnockoutPlatelet-Derived Growth FactorProtein Structure, TertiaryConceptsFocal adhesion kinase activationFocal adhesion kinaseDetergent-resistant membranesMembrane microdomainsSubcellular localization of focal adhesion kinaseLocalization of focal adhesion kinaseSrc activationCell adhesion to extracellular matrix proteinsAdhesion to extracellular matrix proteinsDetergent-resistant membrane microdomainsPlatelet-derived growth factorResponse to different physiological stimuliFluorescence resonance energy transferExtracellular matrix proteinsFAK activationSubcellular localizationCellular processesSubcellular compartmentsResonance energy transferKinase activityPlasma membraneCell adhesionMatrix proteinsFRET responseMicrodomains