2013
Photonic crystal enhanced microscopy for imaging of live cell adhesion
Chen W, Long K, Lu M, Chaudhery V, Yu H, Choi J, Polans J, Zhuo Y, Harley B, Cunningham B. Photonic crystal enhanced microscopy for imaging of live cell adhesion. Analyst 2013, 138: 5886-5894. PMID: 23971078, DOI: 10.1039/c3an01541f.Peer-Reviewed Original ResearchConceptsTime-resolved imagingPhotonic crystalsRelevant time-scalesTime-scalesDrug-induced apoptosisSubmicron resolutionSpatial resolutionMorphological signaturesStem cell differentiationCell-surface interactionsFilopodial extensionsPhotonsCell adhesionCell differentiationDynamic informationCell attachmentLiving cell adhesionMonitor cell behaviorCell behaviorBiological phenomenaResolutionApoptosisEvolutionChemotaxisLabel-freeAntagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2
Sun J, Lu S, Ouyang M, Lin L, Zhuo Y, Liu B, Chien S, Neel B, Wang Y. Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2. Nature Communications 2013, 4: 2037. PMID: 23792876, PMCID: PMC3777412, DOI: 10.1038/ncomms3037.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesEmbryo, MammalianExtracellular Signal-Regulated MAP KinasesFibroblastsFluorescence Resonance Energy TransferGenes, ReporterGRB2 Adaptor ProteinHEK293 CellsHumansKineticsMiceMolecular Sequence DataMutant ProteinsMutationPeptidesPhosphorylationPhosphotyrosineProtein BindingProtein ConformationProtein Tyrosine Phosphatase, Non-Receptor Type 11ConceptsCis-interactionsSH2 domainC-terminal phosphotyrosinesProtein tyrosine phosphatase SHP2Amino acid sequenceTyrosine phosphatase SHP2Adaptor proteinModular domainsModular proteinsTrans-regulationAcid sequenceProtein functionPhosphatase SHP2Evolutionary dynamicsBinding site affinityPhosphotyrosineConformational dynamicsProteinGrb2SH2SequenceSHP2Site affinityAntagonist combinationDominant form
2011
Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer
Seong J, Ouyang M, Kim T, Sun J, Wen P, Lu S, Zhuo Y, Llewellyn N, Schlaepfer D, Guan J, Chien S, Wang Y. Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Nature Communications 2011, 2: 406. PMID: 21792185, PMCID: PMC3373894, DOI: 10.1038/ncomms1414.Peer-Reviewed Original ResearchConceptsFocal adhesion kinase activationFocal adhesion kinaseDetergent-resistant membranesMembrane microdomainsSubcellular localization of focal adhesion kinaseLocalization of focal adhesion kinaseSrc activationCell adhesion to extracellular matrix proteinsAdhesion to extracellular matrix proteinsDetergent-resistant membrane microdomainsPlatelet-derived growth factorResponse to different physiological stimuliFluorescence resonance energy transferExtracellular matrix proteinsFAK activationSubcellular localizationCellular processesSubcellular compartmentsResonance energy transferKinase activityPlasma membraneCell adhesionMatrix proteinsFRET responseMicrodomains