2015
Subcellular and Dynamic Coordination between Src Activity and Cell Protrusion in Microenvironment
Zhuo Y, Qian T, Wu Y, Seong J, Gong Y, Ma H, Wang Y, Lu S. Subcellular and Dynamic Coordination between Src Activity and Cell Protrusion in Microenvironment. Scientific Reports 2015, 5: 12963. PMID: 26261043, PMCID: PMC4531316, DOI: 10.1038/srep12963.Peer-Reviewed Original ResearchMeSH KeywordsCell MovementCell PolarityCell Surface ExtensionsCellular MicroenvironmentEndothelial CellsFluorescence Resonance Energy TransferHumansNeovascularization, PhysiologicSrc-Family KinasesWound HealingConceptsSrc kinase activitySrc activationKinase activityFluorescence resonance energy transfer (FRET)-based biosensorsInitiation of cell migrationNon-polarized cellsCoordinated molecular eventsAutomated computer image analysisMembrane protrusionsCell protrusionsCell polarityProtrusive edgesPolarized cellsMigration of endothelial cellsCell migrationSrcEndothelial cellsMolecular eventsSpatiotemporal coordinationSpatiotemporal controlEndothelial migrationCellsComputer image analysisCell edgeTumor micro-environment
2013
The Effect of Differentiation Induction on FAK and Src Activity in Live HMSCs Visualized by FRET
Liao X, Lu S, Wu Y, Xu W, Zhuo Y, Peng Q, Li B, Zhang L, Wang Y. The Effect of Differentiation Induction on FAK and Src Activity in Live HMSCs Visualized by FRET. PLOS ONE 2013, 8: e72233. PMID: 24015220, PMCID: PMC3754985, DOI: 10.1371/journal.pone.0072233.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationCells, CulturedCSK Tyrosine-Protein KinaseEnzyme ActivationFeedback, PhysiologicalFluorescence Resonance Energy TransferFocal Adhesion Kinase 1HumansMesenchymal Stem CellsProto-Oncogene ProteinsProto-Oncogene Proteins p21(ras)Ras ProteinsSignal TransductionSrc-Family KinasesTissue EngineeringConceptsSrc activationCell differentiationStem cell differentiationInhibiting FAK activityStages of stem cell differentiationPlasma membrane of human mesenchymal stem cellsSrc biosensorFAK activationActivated FAKActivated SrcFate decisionsInduction reagentsFAKSrc signalingKinase activityPlasma membraneSrcRegulation mechanismLiving human mesenchymal stem cellsUp-regulatedHuman mesenchymal stem cellsDifferentiation inductionNeural inductionDifferentiationCellsAntagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2
Sun J, Lu S, Ouyang M, Lin L, Zhuo Y, Liu B, Chien S, Neel B, Wang Y. Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2. Nature Communications 2013, 4: 2037. PMID: 23792876, PMCID: PMC3777412, DOI: 10.1038/ncomms3037.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesEmbryo, MammalianExtracellular Signal-Regulated MAP KinasesFibroblastsFluorescence Resonance Energy TransferGenes, ReporterGRB2 Adaptor ProteinHEK293 CellsHumansKineticsMiceMolecular Sequence DataMutant ProteinsMutationPeptidesPhosphorylationPhosphotyrosineProtein BindingProtein ConformationProtein Tyrosine Phosphatase, Non-Receptor Type 11ConceptsCis-interactionsSH2 domainC-terminal phosphotyrosinesProtein tyrosine phosphatase SHP2Amino acid sequenceTyrosine phosphatase SHP2Adaptor proteinModular domainsModular proteinsTrans-regulationAcid sequenceProtein functionPhosphatase SHP2Evolutionary dynamicsBinding site affinityPhosphotyrosineConformational dynamicsProteinGrb2SH2SequenceSHP2Site affinityAntagonist combinationDominant form
2011
Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer
Seong J, Ouyang M, Kim T, Sun J, Wen P, Lu S, Zhuo Y, Llewellyn N, Schlaepfer D, Guan J, Chien S, Wang Y. Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Nature Communications 2011, 2: 406. PMID: 21792185, PMCID: PMC3373894, DOI: 10.1038/ncomms1414.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell MembraneEnzyme ActivationFluorescence Resonance Energy TransferFocal Adhesion Protein-Tyrosine KinasesMiceMice, KnockoutPlatelet-Derived Growth FactorProtein Structure, TertiaryConceptsFocal adhesion kinase activationFocal adhesion kinaseDetergent-resistant membranesMembrane microdomainsSubcellular localization of focal adhesion kinaseLocalization of focal adhesion kinaseSrc activationCell adhesion to extracellular matrix proteinsAdhesion to extracellular matrix proteinsDetergent-resistant membrane microdomainsPlatelet-derived growth factorResponse to different physiological stimuliFluorescence resonance energy transferExtracellular matrix proteinsFAK activationSubcellular localizationCellular processesSubcellular compartmentsResonance energy transferKinase activityPlasma membraneCell adhesionMatrix proteinsFRET responseMicrodomains