Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer
Seong J, Ouyang M, Kim T, Sun J, Wen P, Lu S, Zhuo Y, Llewellyn N, Schlaepfer D, Guan J, Chien S, Wang Y. Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Nature Communications 2011, 2: 406. PMID: 21792185, PMCID: PMC3373894, DOI: 10.1038/ncomms1414.Peer-Reviewed Original ResearchConceptsFocal adhesion kinase activationFocal adhesion kinaseDetergent-resistant membranesMembrane microdomainsSubcellular localization of focal adhesion kinaseLocalization of focal adhesion kinaseSrc activationCell adhesion to extracellular matrix proteinsAdhesion to extracellular matrix proteinsDetergent-resistant membrane microdomainsPlatelet-derived growth factorResponse to different physiological stimuliFluorescence resonance energy transferExtracellular matrix proteinsFAK activationSubcellular localizationCellular processesSubcellular compartmentsResonance energy transferKinase activityPlasma membraneCell adhesionMatrix proteinsFRET responseMicrodomains