2019
Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells
Dong MB, Wang G, Chow RD, Ye L, Zhu L, Dai X, Park JJ, Kim HR, Errami Y, Guzman CD, Zhou X, Chen KY, Renauer PA, Du Y, Shen J, Lam SZ, Zhou JJ, Lannin DR, Herbst RS, Chen S. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell 2019, 178: 1189-1204.e23. PMID: 31442407, PMCID: PMC6719679, DOI: 10.1016/j.cell.2019.07.044.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBreast NeoplasmsCD8-Positive T-LymphocytesCell Line, TumorClustered Regularly Interspaced Short Palindromic RepeatsCytokinesFemaleHumansImmunologic MemoryImmunotherapyMaleMiceMice, KnockoutNF-kappa BProgrammed Cell Death 1 ReceptorRNA HelicasesRNA, Guide, CRISPR-Cas SystemsTranscriptomeConceptsCRISPR screensTarget discoveryGenome-scale CRISPR screensCD8 TRNA helicase DHX37Vivo CRISPR screensGenetic screenGenome scaleTranscriptomic profilingBiochemical interrogationAntigen-specific CD8 TAnti-tumor immune responseFunctional regulatorTriple-negative breast cancerDHX37Essential roleTim-3PD-1Cytokine productionTumor infiltrationImmunotherapy targetImmunotherapy settingsRegulatorBreast cancerT cells
2015
Herpes Simplex Virus 1 Suppresses the Function of Lung Dendritic Cells via Caveolin-1
Wu B, Geng S, Bi Y, Liu H, Hu Y, Li X, Zhang Y, Zhou X, Zheng G, He B, Wang B. Herpes Simplex Virus 1 Suppresses the Function of Lung Dendritic Cells via Caveolin-1. MSphere 2015, 22: 883-895. PMID: 26018534, PMCID: PMC4519715, DOI: 10.1128/cvi.00170-15.Peer-Reviewed Original ResearchConceptsInducible nitric oxide synthaseDendritic cellsHSV-1 infectionCav-1HSV-1Nitric oxideHerpes simplex virus 1 (HSV-1) infectionLung dendritic cellsSimplex virus 1 infectionLung pathological changesCav-1 knockout miceNitric oxide synthaseVirus-1 infectionWild-type miceHost antiviral responseHerpes simplex virus 1Cav-1 deficiencySimplex virus 1Adoptive transferCaveolin-1Cav-1 knockdownINOS inhibitorOxide synthasePathological changesTherapeutic approaches