2024
Competition and synergy of Arp2/3 and formins in nucleating actin waves
Le Chua X, San Tong C, Su M, Xǔ X, Xiao S, Wu X, Wu M. Competition and synergy of Arp2/3 and formins in nucleating actin waves. Cell Reports 2024, 43: 114423. PMID: 38968072, PMCID: PMC11378572, DOI: 10.1016/j.celrep.2024.114423.Peer-Reviewed Original ResearchActin wavesActin regulatory proteinsActin cytoskeletal networkConstitutively active mutantArp2/3 complexActin assemblyMonomeric actinActive Cdc42Cdc42-interactingCytoskeletal networkArp2/3Active mutantCellular processesRegulatory proteinsUpstream regulatorCdc42ActinPlasma membraneFMNL1Complex feedback loopsForminPhysiological stateDelayed recruitmentFeedback loopGTPaseNonlinear dynamics in phosphoinositide metabolism
Fung S, Xǔ X, Wu M. Nonlinear dynamics in phosphoinositide metabolism. Current Opinion In Cell Biology 2024, 88: 102373. PMID: 38797149, PMCID: PMC11186694, DOI: 10.1016/j.ceb.2024.102373.Peer-Reviewed Original ResearchMetabolic networksPhosphoinositide metabolismNegative feedback loopCellular physiologyEnzymatic functionSignaling complexSignal transductionMembrane dynamicsNetwork motifsMetabolic pathwaysLipid fluxCellular behaviorMolecular circuitsPhosphoinositideFeedforward loopProducts of metabolismMetabolismFeedback loopExperimental challengeNonlinear dynamicsMotifFramework of nonlinear dynamicsTransductionExcited statesComprehensive understandingCollective dynamics of actin and microtubule and its crosstalk mediated by FHDC1
San Tong C, Su M, Sun H, Le Chua X, Xiong D, Guo S, Raj R, Ong N, Lee A, Miao Y, Wu M. Collective dynamics of actin and microtubule and its crosstalk mediated by FHDC1. Frontiers In Cell And Developmental Biology 2024, 11: 1261117. PMID: 38567385, PMCID: PMC10985548, DOI: 10.3389/fcell.2023.1261117.Peer-Reviewed Original ResearchActin wavesActin polymerizationFormin-mediated actin polymerizationDynamics of actinMultiple cellular processesActin nucleationCell cortexCytoskeletal networkCellular processesMicrotubule networkCell divisionMicrotubule depolymerizationActinAntagonistic interplayMicrotubulesMolecular insightsProtein 1CellsCdc42Mast cellsConcurrent releaseDepolymerizationInteractionInhibition
2023
Competition and Synergy of Arp2/3 and Formins in Nucleating Actin Waves.
Le Chua X, Tong CS, Xǔ XJ, Su M, Xiao S, Wu X, Wu M. Competition and Synergy of Arp2/3 and Formins in Nucleating Actin Waves. BioRxiv 2023 PMID: 37745345, DOI: 10.1101/2023.09.13.557508.Peer-Reviewed Original Research In PressPeriodicity, mixed-mode oscillations, and multiple timescales in a phosphoinositide-Rho GTPase network
San Tong C, Xǔ X, Wu M. Periodicity, mixed-mode oscillations, and multiple timescales in a phosphoinositide-Rho GTPase network. Cell Reports 2023, 42: 112857. PMID: 37494180, DOI: 10.1016/j.celrep.2023.112857.Peer-Reviewed Original Research
2021
A kinetic view of clathrin assembly and endocytic cargo sorting
Wu M, Wu X. A kinetic view of clathrin assembly and endocytic cargo sorting. Current Opinion In Cell Biology 2021, 71: 130-138. PMID: 33865229, DOI: 10.1016/j.ceb.2021.02.010.Peer-Reviewed Original Research
2020
Comparative Study of Curvature Sensing Mediated by F-BAR and an Intrinsically Disordered Region of FBP17
Su M, Zhuang Y, Miao X, Zeng Y, Gao W, Zhao W, Wu M. Comparative Study of Curvature Sensing Mediated by F-BAR and an Intrinsically Disordered Region of FBP17. IScience 2020, 23: 101712. PMID: 33205024, PMCID: PMC7649350, DOI: 10.1016/j.isci.2020.101712.Peer-Reviewed Original ResearchMechanobiology in cortical waves and oscillations
Wu M, Liu J. Mechanobiology in cortical waves and oscillations. Current Opinion In Cell Biology 2020, 68: 45-54. PMID: 33039945, DOI: 10.1016/j.ceb.2020.08.017.Peer-Reviewed Original Research
2019
Dynamic instability of clathrin assembly provides proofreading control for endocytosis
Chen Y, Yong J, Martínez-Sánchez A, Yang Y, Wu Y, De Camilli P, Fernández-Busnadiego R, Wu M. Dynamic instability of clathrin assembly provides proofreading control for endocytosis. Journal Of Cell Biology 2019, 218: 3200-3211. PMID: 31451612, PMCID: PMC6781453, DOI: 10.1083/jcb.201804136.Peer-Reviewed Original ResearchConceptsClathrin-coated pitsCell-free reconstitution systemClathrin coat formationSingle-molecule imagingAssembly machineryCargo sortingATPase Hsc70Hsc70 functionClathrin assemblyCoat formationCargo incorporationReconstitution systemDynamic exchangeProofreading mechanismClathrinEndocytosisAssembly rateCargoCheckpointDeconstructing Actin Waves
Wu M. Deconstructing Actin Waves. Structure 2019, 27: 1187-1189. PMID: 31390543, DOI: 10.1016/j.str.2019.07.010.Peer-Reviewed Original Research
2018
Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Yong J, Chen Y, Wu M. Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System. Methods In Molecular Biology 2018, 1847: 177-187. PMID: 30129017, DOI: 10.1007/978-1-4939-8719-1_13.Peer-Reviewed Original ResearchRhythmicity and waves in the cortex of single cells
Yang Y, Wu M. Rhythmicity and waves in the cortex of single cells. Philosophical Transactions Of The Royal Society B Biological Sciences 2018, 373: 20170116. PMID: 29632268, PMCID: PMC5904302, DOI: 10.1098/rstb.2017.0116.Peer-Reviewed Original ResearchConceptsDifferent dynamical statesDynamical stateDynamic systemsForm of oscillationsBasic propertiesSpatial couplingNetwork-centric viewSingle cellsPattern formationWavesCellular functionsTheme issueCell biologyMore mechanistic studiesCellular organizationFunctional importanceDynamic patternsEnigmatic phenomenonMolecular definitionKey parametersOscillationsLight, space, and time in cancer signaling
McPherson PS, Wu M. Light, space, and time in cancer signaling. Molecular Biology Of The Cell 2018, 29: 688-688. PMID: 29535172, PMCID: PMC6003231, DOI: 10.1091/mbc.e17-11-0675.Peer-Reviewed Original ResearchExtracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs
Chen M, Qiu T, Wu J, Yang Y, Wright GD, Wu M, Ge R. Extracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs. Cell Death & Differentiation 2018, 25: 1905-1920. PMID: 29523874, PMCID: PMC6219483, DOI: 10.1038/s41418-018-0092-9.Peer-Reviewed Original ResearchMeSH KeywordsAngiostatinsApoptosisCell MembraneEndocytosisEndoplasmic Reticulum Chaperone BiPEndosomesFibronectinsFluorescence Recovery After PhotobleachingHeat-Shock ProteinsHuman Umbilical Vein Endothelial CellsHumansMicroscopy, FluorescenceMitochondriaNeovascularization, PhysiologicPhosphoproteinsProtein TransportRecombinant ProteinsRNA InterferenceRNA, Small InterferingSodium-Hydrogen ExchangersSynaptosomal-Associated Protein 25ThrombospondinsConceptsLate endosomesEndosomal proteinExtracellular proteinsT-SNARE proteinsCytosolic scaffold proteinsTotal internal reflection fluorescent microscopyPro-apoptotic functionFluorescent microscopyRegulatory factor 1Recycling endosomesRNAi knockdownCargo degradationScaffold proteinSuper-resolution fluorescent microscopyMitochondrial traffickingCell fractionationPlasma membraneIsthminProximity ligationMitochondriaProtein 25ProteinBiochemical analysisAnti-angiogenic proteinFactor 1Membrane shape-mediated wave propagation of cortical protein dynamics
Wu Z, Su M, Tong C, Wu M, Liu J. Membrane shape-mediated wave propagation of cortical protein dynamics. Nature Communications 2018, 9: 136. PMID: 29321558, PMCID: PMC5762918, DOI: 10.1038/s41467-017-02469-1.Peer-Reviewed Original ResearchConceptsMembrane shape changesF-BAR proteinsDomain-containing proteinsPeripheral membrane proteinsProtein lateral diffusionActin machineryProtein recruitmentMembrane proteinsProtein dynamicsCortical proteinsShape changesProtein waveActin wavesUnderappreciated roleProteinMembrane undulationsLateral diffusionSpatial gradientsMachineryCytoplasmImmune cellsRecruitmentAssemblyCells
2017
Pulses and waves of contractility
Wu M. Pulses and waves of contractility. Journal Of Cell Biology 2017, 216: 3899-3901. PMID: 29138250, PMCID: PMC5716294, DOI: 10.1083/jcb.201710079.Peer-Reviewed Original ResearchMitotic Cortical Waves Predict Future Division Sites by Encoding Positional and Size Information
Xiao S, Tong C, Yang Y, Wu M. Mitotic Cortical Waves Predict Future Division Sites by Encoding Positional and Size Information. Developmental Cell 2017, 43: 493-506.e3. PMID: 29161593, DOI: 10.1016/j.devcel.2017.10.023.Peer-Reviewed Original ResearchClathrin Assembly Defines the Onset and Geometry of Cortical Patterning
Yang Y, Xiong D, Pipathsouk A, Weiner OD, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Developmental Cell 2017, 43: 507-521.e4. PMID: 29161594, PMCID: PMC5826602, DOI: 10.1016/j.devcel.2017.10.028.Peer-Reviewed Original ResearchConceptsPlasma membraneClathrin assemblyClathrin functionEndocytic proteinsMembrane traffickingEndocytic machineryN-WASPEndocytic eventsSignal transductionDownstream factorsCell surfaceCortical patterningPattern formationAssemblyMembraneImportant insightsActive processSingle-cell patternFBP17Cdc42ClathrinEndocytosisTransductionCortical pattern formationPhosphatidylinositolFFAR2‐FFAR3 receptor heteromerization modulates short‐chain fatty acid sensing
Ang Z, Xiong D, Wu M, Ding JL. FFAR2‐FFAR3 receptor heteromerization modulates short‐chain fatty acid sensing. The FASEB Journal 2017, 32: 289-303. PMID: 28883043, PMCID: PMC5731126, DOI: 10.1096/fj.201700252rr.Peer-Reviewed Original ResearchMeSH KeywordsBeta-Arrestin 2Calcium SignalingColitisCyclic AMPFatty Acids, VolatileFluorescence Resonance Energy TransferHEK293 CellsHumansMacrophagesObesityP38 Mitogen-Activated Protein KinasesPhosphorylationProtein MultimerizationReceptors, Cell SurfaceReceptors, G-Protein-CoupledRecombinant ProteinsConceptsShort-chain fatty acidsBimolecular fluorescence complementationReceptor heteromerizationGut microbiota-derived short-chain fatty acidsMicrobiota-derived short-chain fatty acidsFluorescence complementationFluorescence resonance energy transferHeterologous expressionFree fatty acid receptor 2Mammalian receptorsFatty acidsFatty acid sensingProximity ligationDrug targetsΒ-arrestinHEK293 cellsPrimary human monocytesResonance energy transferNovel pathwayCytosolic CaP38 phosphorylationHeteromersInflammatory monocytesReceptor 2Receptor heteromersProbing Mammalian Cell Size Homeostasis by Channel-Assisted Cell Reshaping
Varsano G, Wang Y, Wu M. Probing Mammalian Cell Size Homeostasis by Channel-Assisted Cell Reshaping. Cell Reports 2017, 20: 397-410. PMID: 28700941, DOI: 10.1016/j.celrep.2017.06.057.Peer-Reviewed Original ResearchConceptsCell size homeostasisSize homeostasisSize checkpointCell cycle progressionMammalian cell growthG1/SCell sizeCell reshapingMammalian cellsHomeostasis mechanismsCell growthRat basophilic leukemia cellsCell lengthBasophilic leukemia cellsCheckpointHomeostasisCell size measurementsLeukemia cellsAlternative mechanismCellsRAW 264.7 macrophages