2020
Mechanobiology in cortical waves and oscillations
Wu M, Liu J. Mechanobiology in cortical waves and oscillations. Current Opinion In Cell Biology 2020, 68: 45-54. PMID: 33039945, DOI: 10.1016/j.ceb.2020.08.017.Peer-Reviewed Original Research
2019
Dynamic instability of clathrin assembly provides proofreading control for endocytosis
Chen Y, Yong J, Martínez-Sánchez A, Yang Y, Wu Y, De Camilli P, Fernández-Busnadiego R, Wu M. Dynamic instability of clathrin assembly provides proofreading control for endocytosis. Journal Of Cell Biology 2019, 218: 3200-3211. PMID: 31451612, PMCID: PMC6781453, DOI: 10.1083/jcb.201804136.Peer-Reviewed Original ResearchConceptsClathrin-coated pitsCell-free reconstitution systemClathrin coat formationSingle-molecule imagingAssembly machineryCargo sortingATPase Hsc70Hsc70 functionClathrin assemblyCoat formationCargo incorporationReconstitution systemDynamic exchangeProofreading mechanismClathrinEndocytosisAssembly rateCargoCheckpoint
2018
Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Yong J, Chen Y, Wu M. Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System. Methods In Molecular Biology 2018, 1847: 177-187. PMID: 30129017, DOI: 10.1007/978-1-4939-8719-1_13.Peer-Reviewed Original ResearchMembrane shape-mediated wave propagation of cortical protein dynamics
Wu Z, Su M, Tong C, Wu M, Liu J. Membrane shape-mediated wave propagation of cortical protein dynamics. Nature Communications 2018, 9: 136. PMID: 29321558, PMCID: PMC5762918, DOI: 10.1038/s41467-017-02469-1.Peer-Reviewed Original ResearchConceptsMembrane shape changesF-BAR proteinsDomain-containing proteinsPeripheral membrane proteinsProtein lateral diffusionActin machineryProtein recruitmentMembrane proteinsProtein dynamicsCortical proteinsShape changesProtein waveActin wavesUnderappreciated roleProteinMembrane undulationsLateral diffusionSpatial gradientsMachineryCytoplasmImmune cellsRecruitmentAssemblyCells
2017
Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning
Yang Y, Xiong D, Pipathsouk A, Weiner OD, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Developmental Cell 2017, 43: 507-521.e4. PMID: 29161594, PMCID: PMC5826602, DOI: 10.1016/j.devcel.2017.10.028.Peer-Reviewed Original ResearchConceptsPlasma membraneClathrin assemblyClathrin functionEndocytic proteinsMembrane traffickingEndocytic machineryN-WASPEndocytic eventsSignal transductionDownstream factorsCell surfaceCortical patterningPattern formationAssemblyMembraneImportant insightsActive processSingle-cell patternFBP17Cdc42ClathrinEndocytosisTransductionCortical pattern formationPhosphatidylinositolProbing Mammalian Cell Size Homeostasis by Channel-Assisted Cell Reshaping
Varsano G, Wang Y, Wu M. Probing Mammalian Cell Size Homeostasis by Channel-Assisted Cell Reshaping. Cell Reports 2017, 20: 397-410. PMID: 28700941, DOI: 10.1016/j.celrep.2017.06.057.Peer-Reviewed Original ResearchConceptsCell size homeostasisSize homeostasisSize checkpointCell cycle progressionMammalian cell growthG1/SCell sizeCell reshapingMammalian cellsHomeostasis mechanismsCell growthRat basophilic leukemia cellsCell lengthBasophilic leukemia cellsCheckpointHomeostasisCell size measurementsLeukemia cellsAlternative mechanismCellsRAW 264.7 macrophages
2016
Frequency and amplitude control of cortical oscillations by phosphoinositide waves
Xiong D, Xiao S, Guo S, Lin Q, Nakatsu F, Wu M. Frequency and amplitude control of cortical oscillations by phosphoinositide waves. Nature Chemical Biology 2016, 12: 159-166. PMID: 26751515, DOI: 10.1038/nchembio.2000.Peer-Reviewed Original ResearchActinsAnimalsCell Line, TumorCerebral CortexCytoskeletonInositol Polyphosphate 5-PhosphatasesKineticsLeukemia, Basophilic, AcuteLipid MetabolismMast CellsNerve Tissue ProteinsPhosphatidylinositol 3-KinasesPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol PhosphatesPhosphatidylinositolsPhosphoric Monoester HydrolasesRats
2015
Shaping Developing Tissues with Light
Wu M. Shaping Developing Tissues with Light. Developmental Cell 2015, 35: 533-534. PMID: 26651289, DOI: 10.1016/j.devcel.2015.11.020.Peer-Reviewed Original Research
2014
Intracellular Tracking of Single Native Molecules with Electroporation-Delivered Quantum Dots
Sun C, Cao Z, Wu M, Lu C. Intracellular Tracking of Single Native Molecules with Electroporation-Delivered Quantum Dots. Analytical Chemistry 2014, 86: 11403-11409. PMID: 25341054, DOI: 10.1021/ac503363m.Peer-Reviewed Original Research
2013
Calcium oscillations-coupled conversion of actin travelling waves to standing oscillations
Wu M, Wu X, De Camilli P. Calcium oscillations-coupled conversion of actin travelling waves to standing oscillations. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 1339-1344. PMID: 23297209, PMCID: PMC3557052, DOI: 10.1073/pnas.1221538110.Peer-Reviewed Original ResearchConceptsPlasma membraneActin regulatory proteinsActin cytoskeletonActin dynamicsActive Cdc42Cell cortexCell physiologyRegulatory proteinsActin polymerizationDynamic spatial patternsMacromolecular assembliesCalcium oscillationsSpatial patternsActinMembraneFBP17Cdc42CellsCytoskeletonFeedback mechanismProteinPhysiologyAntigen stimulationGlobal fluctuationsMechanism
2012
Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding
Wu M, De Camilli P. Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding. Methods In Cell Biology 2012, 108: 1-18. PMID: 22325595, DOI: 10.1016/b978-0-12-386487-1.00001-8.Peer-Reviewed Original ResearchConceptsPlasma membranePlasma membrane sheetsNative plasma membraneEndocytic budsCell-free assayMembrane buddingEndocytic eventsMolecular dissectionElucidation of mechanismsCytosolic extractsBiological membranesMembrane sheetsEndocytosisExtracellular materialRobust formationMembraneSubsequent incubationVariety of manipulationsReconstitutionCellsExperimental systemPowerful toolFissionBuddingBuds
2010
Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nature Cell Biology 2010, 12: 902-908. PMID: 20729836, PMCID: PMC3338250, DOI: 10.1038/ncb2094.Peer-Reviewed Original ResearchActinsAcyltransferasesAdenosine TriphosphateAnimalsAntibodiesBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCattleCell LineCell MembraneCell Membrane StructuresCell-Free SystemClathrinCoated Pits, Cell-MembraneCytosolDynaminsEndocytosisFatty Acid-Binding ProteinsFibroblastsGuanosine 5'-O-(3-Thiotriphosphate)Guanosine TriphosphateHumansImaging, Three-DimensionalMiceMicroscopy, Electron, TransmissionMicroscopy, FluorescenceModels, BiologicalPotoroidaeRatsReceptors, TransferrinThiazolidines
2007
Differential targeting of secretory lysosomes and recycling endosomes in mast cells revealed by patterned antigen arrays
Wu M, Baumgart T, Hammond S, Holowka D, Baird B. Differential targeting of secretory lysosomes and recycling endosomes in mast cells revealed by patterned antigen arrays. Journal Of Cell Science 2007, 120: 3147-3154. PMID: 17698921, DOI: 10.1242/jcs.007260.Peer-Reviewed Original Research
2005
Lipid segregation and IgE receptor signaling: A decade of progress
Holowka D, Gosse J, Hammond A, Han X, Sengupta P, Smith N, Wagenknecht-Wiesner A, Wu M, Young R, Baird B. Lipid segregation and IgE receptor signaling: A decade of progress. Biochimica Et Biophysica Acta 2005, 1746: 252-259. PMID: 16054713, DOI: 10.1016/j.bbamcr.2005.06.007.Peer-Reviewed Original ResearchConceptsLipid raftsReceptor phosphorylationSrc family kinase LynTransmembrane tyrosine phosphataseLipid segregationPlasma membrane resultsIgE receptor signalingCell surface receptorsActive LynKinase LynTyrosine phosphataseCytoskeletal interactionsSignal transductionRaft environmentMembrane skeletonDisordered regionsReceptor signalingSurface receptorsSegregation of liquidIgE receptorMembrane structureLynPhosphorylationMembrane resultsComplex roleHigh Spatial Resolution Observation of Single-Molecule Dynamics in Living Cell Membranes
Edel J, Wu M, Baird B, Craighead H. High Spatial Resolution Observation of Single-Molecule Dynamics in Living Cell Membranes. Biophysical Journal 2005, 88: l43-l45. PMID: 15821167, PMCID: PMC1305672, DOI: 10.1529/biophysj.105.061937.Peer-Reviewed Original ResearchConceptsZero-mode waveguidesHigh spatial resolution observationsSpatial resolution observationsCell membraneSingle-molecule dynamicsOptical confinementBiological membrane functionResolution observationsCell membrane componentsFluorescent excitationCellular organellesCytosolic poolCellular membranesMembrane componentsSingle moleculesMembrane functionLipid diffusionBiological membranesRat basophilic leukemia cellsLipid bilayersConfocal microscopyConfinementBasophilic leukemia cellsIndividual moleculesEssential building blocks
2004
Visualization of plasma membrane compartmentalization with patterned lipid bilayers
Wu M, Holowka D, Craighead HG, Baird B. Visualization of plasma membrane compartmentalization with patterned lipid bilayers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13798-13803. PMID: 15356342, PMCID: PMC518836, DOI: 10.1073/pnas.0403835101.Peer-Reviewed Original ResearchConceptsLipid raftsLeaflet componentsPlasma membrane compartmentalizationReceptor clustersMembrane structural organizationLipid bilayersFcepsilon receptor ITyrosine phosphorylation activityMembrane compartmentalizationLyn kinaseInner leafletPhosphorylation activityActin polymerizationCellular componentsStructural organizationCell receptorRaftsCell activationReceptor IMast cell receptorsStructural reorganizationKinaseUnique insightsBilayersProtein