2021
A kinetic view of clathrin assembly and endocytic cargo sorting
Wu M, Wu X. A kinetic view of clathrin assembly and endocytic cargo sorting. Current Opinion In Cell Biology 2021, 71: 130-138. PMID: 33865229, DOI: 10.1016/j.ceb.2021.02.010.Peer-Reviewed Original Research
2019
Dynamic instability of clathrin assembly provides proofreading control for endocytosis
Chen Y, Yong J, Martínez-Sánchez A, Yang Y, Wu Y, De Camilli P, Fernández-Busnadiego R, Wu M. Dynamic instability of clathrin assembly provides proofreading control for endocytosis. Journal Of Cell Biology 2019, 218: 3200-3211. PMID: 31451612, PMCID: PMC6781453, DOI: 10.1083/jcb.201804136.Peer-Reviewed Original ResearchConceptsClathrin-coated pitsCell-free reconstitution systemClathrin coat formationSingle-molecule imagingAssembly machineryCargo sortingATPase Hsc70Hsc70 functionClathrin assemblyCoat formationCargo incorporationReconstitution systemDynamic exchangeProofreading mechanismClathrinEndocytosisAssembly rateCargoCheckpoint
2018
Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Yong J, Chen Y, Wu M. Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System. Methods In Molecular Biology 2018, 1847: 177-187. PMID: 30129017, DOI: 10.1007/978-1-4939-8719-1_13.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Membrane StructuresClathrinEndocytosisHumansKineticsMicroscopy, FluorescenceExtracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs
Chen M, Qiu T, Wu J, Yang Y, Wright GD, Wu M, Ge R. Extracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs. Cell Death & Differentiation 2018, 25: 1905-1920. PMID: 29523874, PMCID: PMC6219483, DOI: 10.1038/s41418-018-0092-9.Peer-Reviewed Original ResearchMeSH KeywordsAngiostatinsApoptosisCell MembraneEndocytosisEndoplasmic Reticulum Chaperone BiPEndosomesFibronectinsFluorescence Recovery After PhotobleachingHeat-Shock ProteinsHuman Umbilical Vein Endothelial CellsHumansMicroscopy, FluorescenceMitochondriaNeovascularization, PhysiologicPhosphoproteinsProtein TransportRecombinant ProteinsRNA InterferenceRNA, Small InterferingSodium-Hydrogen ExchangersSynaptosomal-Associated Protein 25ThrombospondinsConceptsLate endosomesEndosomal proteinExtracellular proteinsT-SNARE proteinsCytosolic scaffold proteinsTotal internal reflection fluorescent microscopyPro-apoptotic functionFluorescent microscopyRegulatory factor 1Recycling endosomesRNAi knockdownCargo degradationScaffold proteinSuper-resolution fluorescent microscopyMitochondrial traffickingCell fractionationPlasma membraneIsthminProximity ligationMitochondriaProtein 25ProteinBiochemical analysisAnti-angiogenic proteinFactor 1
2017
Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning
Yang Y, Xiong D, Pipathsouk A, Weiner OD, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Developmental Cell 2017, 43: 507-521.e4. PMID: 29161594, PMCID: PMC5826602, DOI: 10.1016/j.devcel.2017.10.028.Peer-Reviewed Original ResearchConceptsPlasma membraneClathrin assemblyClathrin functionEndocytic proteinsMembrane traffickingEndocytic machineryN-WASPEndocytic eventsSignal transductionDownstream factorsCell surfaceCortical patterningPattern formationAssemblyMembraneImportant insightsActive processSingle-cell patternFBP17Cdc42ClathrinEndocytosisTransductionCortical pattern formationPhosphatidylinositol
2012
Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding
Wu M, De Camilli P. Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding. Methods In Cell Biology 2012, 108: 1-18. PMID: 22325595, DOI: 10.1016/b978-0-12-386487-1.00001-8.Peer-Reviewed Original ResearchConceptsPlasma membranePlasma membrane sheetsNative plasma membraneEndocytic budsCell-free assayMembrane buddingEndocytic eventsMolecular dissectionElucidation of mechanismsCytosolic extractsBiological membranesMembrane sheetsEndocytosisExtracellular materialRobust formationMembraneSubsequent incubationVariety of manipulationsReconstitutionCellsExperimental systemPowerful toolFissionBuddingBuds
2010
Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nature Cell Biology 2010, 12: 902-908. PMID: 20729836, PMCID: PMC3338250, DOI: 10.1038/ncb2094.Peer-Reviewed Original ResearchActinsAcyltransferasesAdenosine TriphosphateAnimalsAntibodiesBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCattleCell LineCell MembraneCell Membrane StructuresCell-Free SystemClathrinCoated Pits, Cell-MembraneCytosolDynaminsEndocytosisFatty Acid-Binding ProteinsFibroblastsGuanosine 5'-O-(3-Thiotriphosphate)Guanosine TriphosphateHumansImaging, Three-DimensionalMiceMicroscopy, Electron, TransmissionMicroscopy, FluorescenceModels, BiologicalPotoroidaeRatsReceptors, TransferrinThiazolidines