2021
Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps
Chen B, Scurrah CR, McKinley ET, Simmons AJ, Ramirez-Solano MA, Zhu X, Markham NO, Heiser CN, Vega PN, Rolong A, Kim H, Sheng Q, Drewes JL, Zhou Y, Southard-Smith AN, Xu Y, Ro J, Jones AL, Revetta F, Berry LD, Niitsu H, Islam M, Pelka K, Hofree M, Chen JH, Sarkizova S, Ng K, Giannakis M, Boland GM, Aguirre AJ, Anderson AC, Rozenblatt-Rosen O, Regev A, Hacohen N, Kawasaki K, Sato T, Goettel JA, Grady WM, Zheng W, Washington MK, Cai Q, Sears CL, Goldenring JR, Franklin JL, Su T, Huh WJ, Vandekar S, Roland JT, Liu Q, Coffey RJ, Shrubsole MJ, Lau KS. Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell 2021, 184: 6262-6280.e26. PMID: 34910928, PMCID: PMC8941949, DOI: 10.1016/j.cell.2021.11.031.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAdenomaAdultAgedAnimalsCarcinogenesisCell DeathCell DifferentiationColonic PolypsColorectal NeoplasmsDisease ProgressionFemaleGene Expression Regulation, NeoplasticGene Regulatory NetworksGenetic HeterogeneityHumansMaleMiceMiddle AgedMutationNeoplastic Stem CellsReproducibility of ResultsRNA-SeqSingle-Cell AnalysisTumor MicroenvironmentConceptsHuman colorectal polypsColorectal cancerColorectal polypsPrevention of CRCMicrosatellite-unstable colorectal cancersUnstable colorectal cancersGastric metaplasiaImmune microenvironmentTumor cell differentiation statusImmune cellsPrecision surveillancePrecursor polypsSerrated polypsConventional adenomasStem cell propertiesMalignant progressionImmunogenic potentialPolypsTumor cellsTherapeutic insightsCell differentiation statusCellular originMetaplasiaAdenomasMolecular heterogeneity
2020
A smooth muscle‐derived, Braf‐driven mouse model of gastrointestinal stromal tumor (GIST): evidence for an alternative GIST cell‐of‐origin
Kondo J, Huh WJ, Franklin JL, Heinrich MC, Rubin BP, Coffey RJ. A smooth muscle‐derived, Braf‐driven mouse model of gastrointestinal stromal tumor (GIST): evidence for an alternative GIST cell‐of‐origin. The Journal Of Pathology 2020, 252: 441-450. PMID: 32944951, PMCID: PMC7802691, DOI: 10.1002/path.5552.Peer-Reviewed Original ResearchConceptsGastrointestinal stromal tumorsSmooth muscle cellsICC hyperplasiaMuscle cellsTyrosine kinase inhibitor imatinibFrequent driver eventsCommon mesenchymal tumorsSmooth muscle cell progenitorsDevelopment of GISTsKinase inhibitor imatinibLoss of Trp53ICC-DMPGut motilityStromal tumorsMesenchymal tumorsMouse modelInhibitor imatinibInterstitial cellsMutant BRAFBRAF expressionTumorsBRAFHyperplasiaCell progenitorsDriver events
2013
Evolution of the human gastrokine locus and confounding factors regarding the pseudogenicity of GKN3
Geahlen JH, Lapid C, Thorell K, Nikolskiy I, Huh WJ, Oates EL, Lennerz JK, Tian X, Weis VG, Khurana SS, Lundin SB, Templeton AR, Mills JC. Evolution of the human gastrokine locus and confounding factors regarding the pseudogenicity of GKN3. Physiological Genomics 2013, 45: 667-683. PMID: 23715263, PMCID: PMC3742967, DOI: 10.1152/physiolgenomics.00169.2012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsComputational BiologyDNA PrimersEvolution, MolecularFluorescent Antibody TechniqueGastric MucosaGenetic LociGenetics, PopulationGenotypeHaplotypesHumansLikelihood FunctionsMacaca mulattaMembrane ProteinsMiceMice, Inbred C57BLMicroarray AnalysisMicroscopy, ConfocalModels, GeneticMutationPhylogenyPolymorphism, Single NucleotidePseudogenesRacial GroupsSelection, GeneticSpecies SpecificitySus scrofaConceptsSelective sweepsHuman genetic locusGenome evolutionGenomic neighborsSplice donor siteDirectional selectionHuman genomeGenetic lociBp downstreamEuropean genesGKN3Gastric mucous neck cellsStop codon mutationThird memberHuman populationGenesCodon mutationLociGKN2Haplotype analysisMucous neck cellsMutationsAfrican populationsNeck cellsGKN1