A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo
Yang YP, Ma H, Starchenko A, Huh WJ, Li W, Hickman FE, Zhang Q, Franklin JL, Mortlock DP, Fuhrmann S, Carter BD, Ihrie RA, Coffey RJ. A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo. Cell Reports 2017, 19: 1257-1267. PMID: 28494873, PMCID: PMC5517093, DOI: 10.1016/j.celrep.2017.04.048.Peer-Reviewed Original ResearchConceptsEGF receptorEGFR localizationEndogenous regulatory elementsDistinct cell populationsReporter miceGenome editingEGFR internalizationRegulatory elementsCRISPR/C-terminusEndogenous EGFRFluorescent reportersDifferentiated compartmentVillus compartmentTraffickingCell populationsReporterExpressionEGFR expressionDisease statesDistinct patternsCompartmentsAdjacent epitheliumIntestinal tumorsAdult mice