XBP1 Controls Maturation of Gastric Zymogenic Cells by Induction of MIST1 and Expansion of the Rough Endoplasmic Reticulum
Huh WJ, Esen E, Geahlen JH, Bredemeyer AJ, Lee A, Shi G, Konieczny SF, Glimcher LH, Mills JC. XBP1 Controls Maturation of Gastric Zymogenic Cells by Induction of MIST1 and Expansion of the Rough Endoplasmic Reticulum. Gastroenterology 2010, 139: 2038-2049. PMID: 20816838, PMCID: PMC2997137, DOI: 10.1053/j.gastro.2010.08.050.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsCell DifferentiationCell LineChief Cells, GastricDNA-Binding ProteinsEndoplasmic Reticulum, RoughIntegrasesMiceMice, KnockoutMicroscopy, Electron, TransmissionPromoter Regions, GeneticRegulatory Factor X Transcription FactorsSecretory VesiclesStem CellsTranscription FactorsX-Box Binding Protein 1ConceptsEndoplasmic reticulumZymogenic cellsRough endoplasmic reticulumNC markersGastric zymogenic cellsTranscription factor XBP1Large secretory vesiclesLamellar rough endoplasmic reticulumAbsence of XBP1Transcription factor MIST1Cell shape abnormalitiesCre-loxP systemTranscriptional regulationChromatin immunoprecipitationTranscriptional activationSecretory vesiclesGastric cell linesMist1Neck cellsXBP1Cell typesImmunoblot analysisCell linesQuantitative reverse transcriptase-polymerase chain reactionMIST1 expression