2016
Hepatocyte nuclear factor 4α is required for cell differentiation and homeostasis in the adult mouse gastric epithelium
Moore BD, Khurana SS, Huh WJ, Mills JC. Hepatocyte nuclear factor 4α is required for cell differentiation and homeostasis in the adult mouse gastric epithelium. AJP Gastrointestinal And Liver Physiology 2016, 311: g267-g275. PMID: 27340127, PMCID: PMC5007292, DOI: 10.1152/ajpgi.00195.2016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsBinding SitesCell DifferentiationCell LineCell ProliferationEpithelial CellsGastric MucosaGene Expression RegulationGenotypeHepatocyte Nuclear Factor 4HomeostasisHumansMice, KnockoutPhenotypePromoter Regions, GeneticSignal TransductionTransfectionX-Box Binding Protein 1ConceptsZymogenic chief cellsAdult mouse stomachX-box binding protein 1Gastric unitsRole of HNF4αStem cell zoneHepatocyte nuclear factor 4 alphaLoss of HNF4αBinding protein 1Nuclear factor 4 alphaHepatocyte nuclear factor 4αHuman gastric cancer cellsMouse stomachTranscriptional regulatorsChromatin immunoprecipitationNuclear factor 4αGastric cancer cellsXBP1 mRNAHNF4α proteinTranscription factorsUpstream regulationMouse gastric epitheliumHuman gastric cellsCell differentiationAdditional phenotypes
2010
Inducible activation of Cre recombinase in adult mice causes gastric epithelial atrophy, metaplasia, and regenerative changes in the absence of “floxed” alleles
Huh WJ, Mysorekar IU, Mills JC. Inducible activation of Cre recombinase in adult mice causes gastric epithelial atrophy, metaplasia, and regenerative changes in the absence of “floxed” alleles. AJP Gastrointestinal And Liver Physiology 2010, 299: g368-g380. PMID: 20413717, PMCID: PMC3774481, DOI: 10.1152/ajpgi.00021.2010.Peer-Reviewed Original ResearchConceptsInduction of CreGastric epithelial stem cellsSpasmolytic polypeptide-expressing metaplasiaFoci of hyperplasiaSubset of miceTdT-mediated dUTP nick end labelingRegenerative capacityDUTP nick end labelingNick end labelingStem cellsAntral polypsDNA damage markerChicken actin promoterEpithelial atrophyStandard dosesGastric bodyPositive apoptosisEpithelial stem cellsComplete healingProfound atrophyGastric mucosaDamage markersAdult miceLoxP-flanked allelesSevere injuries