2017
A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo
Yang YP, Ma H, Starchenko A, Huh WJ, Li W, Hickman FE, Zhang Q, Franklin JL, Mortlock DP, Fuhrmann S, Carter BD, Ihrie RA, Coffey RJ. A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo. Cell Reports 2017, 19: 1257-1267. PMID: 28494873, PMCID: PMC5517093, DOI: 10.1016/j.celrep.2017.04.048.Peer-Reviewed Original ResearchConceptsEGF receptorEGFR localizationEndogenous regulatory elementsDistinct cell populationsReporter miceGenome editingEGFR internalizationRegulatory elementsCRISPR/C-terminusEndogenous EGFRFluorescent reportersDifferentiated compartmentVillus compartmentTraffickingCell populationsReporterExpressionEGFR expressionDisease statesDistinct patternsCompartmentsAdjacent epitheliumIntestinal tumorsAdult mice
2010
Inducible activation of Cre recombinase in adult mice causes gastric epithelial atrophy, metaplasia, and regenerative changes in the absence of “floxed” alleles
Huh WJ, Mysorekar IU, Mills JC. Inducible activation of Cre recombinase in adult mice causes gastric epithelial atrophy, metaplasia, and regenerative changes in the absence of “floxed” alleles. AJP Gastrointestinal And Liver Physiology 2010, 299: g368-g380. PMID: 20413717, PMCID: PMC3774481, DOI: 10.1152/ajpgi.00021.2010.Peer-Reviewed Original ResearchConceptsInduction of CreGastric epithelial stem cellsSpasmolytic polypeptide-expressing metaplasiaFoci of hyperplasiaSubset of miceTdT-mediated dUTP nick end labelingRegenerative capacityDUTP nick end labelingNick end labelingStem cellsAntral polypsDNA damage markerChicken actin promoterEpithelial atrophyStandard dosesGastric bodyPositive apoptosisEpithelial stem cellsComplete healingProfound atrophyGastric mucosaDamage markersAdult miceLoxP-flanked allelesSevere injuries