2001
The Sonic Hedgehog Receptor Patched Associates with Caveolin-1 in Cholesterol-rich Microdomains of the Plasma Membrane* 210
Karpen H, Bukowski J, Hughes T, Gratton J, Sessa W, Gailani M. The Sonic Hedgehog Receptor Patched Associates with Caveolin-1 in Cholesterol-rich Microdomains of the Plasma Membrane* 210. Journal Of Biological Chemistry 2001, 276: 19503-19511. PMID: 11278759, DOI: 10.1074/jbc.m010832200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesBlotting, WesternCaveolin 1CaveolinsCell MembraneCholesterolCOS CellsDNA, ComplementaryDrosophila ProteinsElectrophoresis, Polyacrylamide GelGlutathione TransferaseHumansImmunohistochemistryMembrane MicrodomainsMembrane ProteinsMicroscopy, ConfocalModels, BiologicalMolecular Sequence DataMutationPatched ReceptorsPrecipitin TestsProtein BindingProtein Structure, TertiaryProtein TransportReceptors, Cell SurfaceReceptors, G-Protein-CoupledRecombinant Fusion ProteinsSignal TransductionSmoothened ReceptorSubcellular FractionsTime FactorsConceptsCholesterol-rich microdomainsRaft microdomainsCaveolin-1Receptor complexEarly embryonic patterningFractionation studiesHedgehog receptor complexCaveolin-enriched microdomainsBuoyant density fractionsEmbryonic patterningHh proteinsLipid raftsSubcellular localizationPlasma membranePatchedPlasmalemmal cholesterolProtein experimentsImmunoprecipitation studiesSmoothenedMicrodomainsConfocal microscopyImmunocytochemistry dataComplexesMembraneDrosophila
1999
Caveolins, Liquid-Ordered Domains, and Signal Transduction
Smart E, Graf G, McNiven M, Sessa W, Engelman J, Scherer P, Okamoto T, Lisanti M. Caveolins, Liquid-Ordered Domains, and Signal Transduction. Molecular And Cellular Biology 1999, 19: 7289-7304. PMID: 10523618, PMCID: PMC84723, DOI: 10.1128/mcb.19.11.7289.Peer-Reviewed Original Research
1998
Biological Action of Leptin as an Angiogenic Factor
Sierra-Honigmann M, Nath A, Murakami C, Garcı́a-Cardeña G, Papapetropoulos A, Sessa W, Madge L, Schechner J, Schwabb M, Polverini P, Flores-Riveros J. Biological Action of Leptin as an Angiogenic Factor. Science 1998, 281: 1683-1686. PMID: 9733517, DOI: 10.1126/science.281.5383.1683.Peer-Reviewed Original ResearchMeSH KeywordsAdipocytesAmino Acid SequenceAnimalsCarrier ProteinsCells, CulturedCorneal NeovascularizationDNA-Binding ProteinsEndothelial Growth FactorsEndothelium, VascularEnergy MetabolismHumansLeptinLipid MetabolismLymphokinesMolecular Sequence DataNeovascularization, PhysiologicPhosphorylationProteinsRatsRats, ZuckerReceptors, Cell SurfaceReceptors, LeptinSTAT3 Transcription FactorTrans-ActivatorsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsFa/fa Zucker ratFunctional leptin receptorHuman endothelial cellsNormal ratsFood intakeLeptin receptorVascular endotheliumZucker ratsLeptinOb-RbAngiogenic factorsEndothelial cellsAngiogenic activityPrimary culturesEnergy expenditureBiological actionsHuman vasculatureVivo assaysRatsCorneaReceptorsPhysiological mechanisms
1997
Dissecting the Interaction between Nitric Oxide Synthase (NOS) and Caveolin FUNCTIONAL SIGNIFICANCE OF THE NOS CAVEOLIN BINDING DOMAININ VIVO *
Garcı́a-Cardeña G, Martasek P, Masters B, Skidd P, Couet J, Li S, Lisanti M, Sessa W. Dissecting the Interaction between Nitric Oxide Synthase (NOS) and Caveolin FUNCTIONAL SIGNIFICANCE OF THE NOS CAVEOLIN BINDING DOMAININ VIVO *. Journal Of Biological Chemistry 1997, 272: 25437-25440. PMID: 9325253, DOI: 10.1074/jbc.272.41.25437.Peer-Reviewed Original ResearchConceptsCaveolin-1Peripheral membrane proteinsInteraction of eNOSC-terminal tailAmino acids 310Direct interactionCo-transfection experimentsSite-directed mutagenesisNovel functional roleEndothelial nitric oxide synthaseMolecular chaperonesCytoplasmic domainCaveolin isoformsDeletion mutantsMammalian cellsEndothelial cell lysatesGlutathione S-transferaseMembrane proteinsCaveolin-2Coat proteinNegative regulationCaveolin-3Endothelial cellsDirect bindingGolgi region
1994
The Nitric Oxide Synthase Family of Proteins
Sessa W. The Nitric Oxide Synthase Family of Proteins. Journal Of Vascular Research 1994, 31: 131-143. PMID: 7511942, DOI: 10.1159/000159039.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid OxidoreductasesAmino Acid SequenceAnimalsArginineCardiovascular SystemCloning, MolecularConsensus SequenceDNAGene Expression Regulation, EnzymologicHumansImmune SystemMolecular Sequence DataNervous SystemNitric OxideNitric Oxide SynthasePhosphorylationSequence AlignmentSequence Homology, Amino AcidConceptsCytochrome P450 reductase domainNitric oxide synthaseP450 reductase domainNitric oxide synthase (NOS) familySynthase familyMammalian enzymeSeparate genesReductase domainMolecular biologyNovel familyIsoforms of NOSImportant familyNitric oxideProteinEndothelial nitric oxide synthaseEnzyme nitric oxide synthaseGuanidino nitrogenImportant bio-regulatory moleculeOxide synthaseFamilyL-arginineCardiovascular systemRecent advancesGenesEnzymology