2000
Simvastatin upregulates coronary vascular endothelial nitric oxide production in conscious dogs
Mital S, Zhang X, Zhao G, Bernstein R, Smith C, Fulton D, Sessa W, Liao J, Hintze T. Simvastatin upregulates coronary vascular endothelial nitric oxide production in conscious dogs. AJP Heart And Circulatory Physiology 2000, 279: h2649-h2657. PMID: 11087217, DOI: 10.1152/ajpheart.2000.279.6.h2649.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenosineAnimalsAnticholesteremic AgentsConsciousnessCoronary CirculationDogsEndothelium, VascularEnzyme InhibitorsGene Expression Regulation, EnzymologicHeart RateIn Vitro TechniquesMicrocirculationMyocardiumNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIINitritesNitroglycerinOxygen ConsumptionRNA, MessengerSimvastatinVasodilationVasodilator AgentsVeratrineConceptsEndothelial nitric oxide synthaseCoronary blood flowCoronary vasodilationConscious dogsSimvastatin administrationVascular endothelial nitric oxide productionVascular endothelial nitric oxide synthaseNO productionEndothelial nitric oxide productionEndothelium-independent vasodilatorCoronary vascular endotheliumShort-term administrationLipid-lowering effectsNitric oxide synthaseEndothelial NO productionMyocardial oxygen consumptionNitric oxide productionNO-dependent regulationPlasma nitrateGeneral anesthesiaENOS proteinCoronary microvesselsOxide synthaseMongrel dogsENOS mRNAAcute modulation of endothelial Akt/PKB activity alters nitric oxide–dependent vasomotor activity in vivo
Luo Z, Fujio Y, Kureishi Y, Rudic R, Daumerie G, Fulton D, Sessa W, Walsh K. Acute modulation of endothelial Akt/PKB activity alters nitric oxide–dependent vasomotor activity in vivo. Journal Of Clinical Investigation 2000, 106: 493-499. PMID: 10953024, PMCID: PMC380252, DOI: 10.1172/jci9419.Peer-Reviewed Original ResearchConceptsDN-AktEndothelial cell nitric oxide synthaseMyr-AktSerine/threonine protein kinase AktProtein kinase AktDominant-negative AktNitric oxideVasomotor toneFemoral arteryAkt functionReplication-defective adenoviral constructKinase AktActive AktEndothelium-dependent vasodilatationKey regulatorEndothelium-independent vasodilatorEndothelium-dependent vasomotionRabbit femoral artery modelNitric oxide synthaseAorta ex vivoImportant regulatorGene transferDoppler flow measurementsAktENOS inhibitorTemporal Events Underlying Arterial Remodeling After Chronic Flow Reduction in Mice
Rudic R, Bucci M, Fulton D, Segal S, Sessa W. Temporal Events Underlying Arterial Remodeling After Chronic Flow Reduction in Mice. Circulation Research 2000, 86: 1160-1166. PMID: 10850968, DOI: 10.1161/01.res.86.11.1160.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarotid Artery, CommonCell DeathDrug CombinationsIn Vitro TechniquesMaleMiceMice, Inbred C57BLMuscle, Smooth, VascularNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIRegional Blood FlowTime FactorsTunica MediaVasodilator AgentsVasomotor SystemConceptsLeft common carotid arteryRight common carotid arteryCommon carotid arteryCarotid arteryBlood flowLeft external carotid arteryEndothelial NO synthase (eNOS) functionEndothelial NO synthase (eNOS) mRNAExternal carotid arteryNO synthase mRNANitrovasodilator sodium nitroprussideAcute ligationEndothelial dysfunctionArterial remodelingControl arteriesVascular remodelingAdult miceSodium nitroprussideDay 7Structural remodelingArteryLuminal remodelingMarked reductionProtein levelsMice
1999
Hsp90 regulation of endothelial nitric oxide synthase contributes to vascular control in portal hypertension
Shah V, Wiest R, Garcia-Cardena G, Cadelina G, Groszmann R, Sessa W. Hsp90 regulation of endothelial nitric oxide synthase contributes to vascular control in portal hypertension. American Journal Of Physiology 1999, 277: g463-g468. PMID: 10444461, DOI: 10.1152/ajpgi.1999.277.2.g463.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsBenzoquinonesBlood VesselsHSP90 Heat-Shock ProteinsHypertension, PortalIn Vitro TechniquesLactams, MacrocyclicMaleMethoxamineMicrocirculationNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIQuinonesRatsRats, Sprague-DawleySplanchnic CirculationTissue DistributionVasoconstrictor AgentsVasodilationVasodilator AgentsConceptsEndothelial nitric oxide synthasePortal vein ligationNitric oxide synthasePortal hypertensionMesenteric vasculatureOxide synthaseNormal animalsACh-dependent vasodilationExperimental portal hypertensionExcessive NO productionNO-dependent responsesNOS catalytic activityDependent vasodilationVein ligationVascular controlMesenteric circulationPVL animalsMesenteric vesselsHeat shock protein 90Sodium nitroprussideNO productionEndothelial liningHypertensionShock protein 90Methoxamine
1993
20-Hydroxyeicosatetraenoic acid is an endothelium-dependent vasoconstrictor in rabbit arteries
Escalante B, Omata K, Sessa W, Lee S, Falck J, Schwartzman M. 20-Hydroxyeicosatetraenoic acid is an endothelium-dependent vasoconstrictor in rabbit arteries. European Journal Of Pharmacology 1993, 235: 1-7. PMID: 8519270, DOI: 10.1016/0014-2999(93)90812-v.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicArachidonic AcidCarotid ArteriesCattleCells, CulturedDose-Response Relationship, DrugEndothelium, VascularHydroxyeicosatetraenoic AcidsIn Vitro TechniquesMaleMesenteric ArteriesProstaglandin-Endoperoxide SynthasesRabbitsRenal ArteryVasoconstrictionVasoconstrictor AgentsConceptsBlood vesselsEndothelium-dependent vasoconstrictorEndothelial cyclooxygenaseRegulation of hemostasisVasoconstrictor metabolitesContractile potencyContractile effectAortic ringsVascular tonePlatelet functionMuscular arteriesRabbit arteriesArachidonate metabolitesPlatelet aggregationCyclooxygenaseArachidonic acidArteryBlood cellsRabbit kidneySeminal vesiclesEicosatetraenoic acidMajor arachidonate metabolitePotencyRam seminal vesiclesPresent study
1991
Human Polymorphonuclear Leukocytes Generate and Degrade Endothelin-1 by Two Distinct Neutral Proteases
Sessa W, Kaw S, Zembowicz A, Änggård E, Hecker M, Vane J. Human Polymorphonuclear Leukocytes Generate and Degrade Endothelin-1 by Two Distinct Neutral Proteases. Journal Of Cardiovascular Pharmacology 1991, 17: s34-38. PMID: 1725374, DOI: 10.1097/00005344-199100177-00010.Peer-Reviewed Original ResearchConceptsET-1Big endothelinEndothelin-1Important pathophysiologic implicationsHuman polymorphonuclear leukocytesFMLP-activated PMNHuman big endothelinContractile factorsPMN infiltrationLeukocyte cathepsin GContractile activityPathophysiologic implicationsActivated PMNsPolymorphonuclear leukocytesNeutral proteaseDisease statesCell-free supernatantCathepsin GPMNPhosphoramidonMetabolismThe biosynthesis of endothelin-1 by human polymorphonuclear leukocytes
Sessa W, Kaw S, Hecker M, Vane J. The biosynthesis of endothelin-1 by human polymorphonuclear leukocytes. Biochemical And Biophysical Research Communications 1991, 174: 613-618. PMID: 1993057, DOI: 10.1016/0006-291x(91)91461-k.Peer-Reviewed Original Research
1990
L-Glutamine inhibits the release of endothelium-derived relaxing factor from the rabbit aorta
Swierkosz T, Mitchell J, Sessa W, Hecker M, Vane J. L-Glutamine inhibits the release of endothelium-derived relaxing factor from the rabbit aorta. Biochemical And Biophysical Research Communications 1990, 172: 143-148. PMID: 2222463, DOI: 10.1016/s0006-291x(05)80184-6.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsAortaEndothelium, VascularGlutamineIn Vitro TechniquesIsomerismKineticsMaleMuscle, Smooth, VascularNitric OxideRabbitsConceptsRelease of EDRFRabbit aortic stripsAortic stripsRabbit aortaL-arginineL-ArgACh-induced relaxationEndothelium-dependent relaxationL-GlnL-Arg levelsIntact blood vesselsEDRF biosynthesisAortic tissueConsecutive infusionsEDRFL-glutamineAortaEndothelial cellsInitial equilibration periodBlood vesselsD-ArgInhibitory effectInfusionPresent studyAmino acid L-glutamineCytochrome P450-Dependent Arachidonic Acid Metabolites, 19− and 20-Hydroxyeicosatetraenoic Acids, Enhance Sodium-Potassium ATPase Activity in Vascular Smooth Muscle
Escalante B, Sessa W, Falck JR, Yadagiri P, Schwartzman M. Cytochrome P450-Dependent Arachidonic Acid Metabolites, 19− and 20-Hydroxyeicosatetraenoic Acids, Enhance Sodium-Potassium ATPase Activity in Vascular Smooth Muscle. Journal Of Cardiovascular Pharmacology 1990, 16: 438-443. PMID: 1700215, DOI: 10.1097/00005344-199009000-00013.Peer-Reviewed Original ResearchConceptsCytochrome P450-dependent arachidonic acid metabolitesPotassium-induced relaxationArachidonic acid metabolitesAcid metabolitesOuabain-sensitive 86Rb uptakeSodium-potassium ATPase activityRat aortic ringsProstaglandin-like materialVascular smooth muscleDose-dependent fashionEffect of omegaAortic ringsSmooth muscleStimulatory effectInhibitory effectATPase activityPresent studyMetabolitesCyclooxygenaseIndomethacinRelease of a neutrophil‐derived vascoconstrictor agent which augments platelet‐induced contractions of blood vessels in vitro
Sessa W, Mullane K. Release of a neutrophil‐derived vascoconstrictor agent which augments platelet‐induced contractions of blood vessels in vitro. British Journal Of Pharmacology 1990, 99: 553-559. PMID: 2110018, PMCID: PMC1917347, DOI: 10.1111/j.1476-5381.1990.tb12967.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicBlood PlateletsCalcimycinIn Vitro TechniquesLipoxygenase InhibitorsMaleMolecular WeightMuscle ContractionMuscle TonusMuscle, Smooth, VascularNeutrophilsN-Formylmethionine Leucyl-PhenylalanineRabbitsSerotoninSerotonin AntagonistsTetradecanoylphorbol AcetateVasoconstrictor AgentsConceptsContractile factorsVascular toneBlood vesselsEnd-organ antagonistNeutrophil-derived productsPlatelet-induced contractionsConcentration-dependent contractionsInhibition of contractionRat stomach stripCyclo-oxygenase enzymeTimes more plateletsRabbit thoracic aortaPhorbol myristate acetateFree radical scavengerContractile responseInhibitors of lipoxygenaseOrgan bathAngiotensin IIReceptor antagonistStomach stripIntact endotheliumNeutrophil activationThoracic aortaActivated neutrophilsDesArg9-bradykinin