2001
Distinction between signaling mechanisms in lipid rafts vs. caveolae
Sowa G, Pypaert M, Sessa W. Distinction between signaling mechanisms in lipid rafts vs. caveolae. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 14072-14077. PMID: 11707586, PMCID: PMC61169, DOI: 10.1073/pnas.241409998.Peer-Reviewed Original ResearchConceptsCav-1Raft domainsLipid raftsCholesterol-rich lipid raft domainsLipid raft domainsCaveolae assemblyEndothelial nitric oxide synthaseCaveolae biogenesisAcylated proteinsSignal transductionSpatial regulationPlasma membraneNegative regulationCaveolin-1CaveolaeFirst clear exampleRaftsPhysical interactionProteinCellsRegulationENOS functionBiogenesisDomainClear exampleCan modulation of endothelial nitric oxide synthase explain the vasculoprotective actions of statins?
Sessa W. Can modulation of endothelial nitric oxide synthase explain the vasculoprotective actions of statins? Trends In Molecular Medicine 2001, 7: 189-191. PMID: 11325618, DOI: 10.1016/s1471-4914(01)01985-2.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseMainstay of therapyCoronary artery diseaseLipid-lowering effectsNitric oxide synthaseNitric oxide synthesisClass of drugsNitric oxide releaseArtery diseaseVasculoprotective actionsOxide synthaseBeneficial actionsOxide synthesisOxide releaseCellular mechanismsStatinsDrugsRecent insightsTherapyMainstayDisease
2000
Direct Interaction between Endothelial Nitric-oxide Synthase and Dynamin-2 IMPLICATIONS FOR NITRIC-OXIDE SYNTHASE FUNCTION*
Cao S, Yao J, McCabe T, Yao Q, Katusic Z, Sessa W, Shah V. Direct Interaction between Endothelial Nitric-oxide Synthase and Dynamin-2 IMPLICATIONS FOR NITRIC-OXIDE SYNTHASE FUNCTION*. Journal Of Biological Chemistry 2000, 276: 14249-14256. PMID: 11120737, DOI: 10.1074/jbc.m006258200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaBlotting, WesternCalcimycinCattleCell LineDose-Response Relationship, DrugDynamin IDynaminsEndothelium, VascularGlutathione TransferaseGolgi ApparatusGTP PhosphohydrolasesIonophoresKineticsMicroscopy, ConfocalMicroscopy, FluorescenceNitric Oxide SynthaseNitric Oxide Synthase Type IIIPrecipitin TestsProtein BindingProtein BiosynthesisRatsRecombinant Fusion ProteinsTransfectionConceptsDynamin 2Bovine aortic endothelial cellsRecombinant eNOS proteinDirect protein-protein interactionDouble-label confocal immunofluorescenceProtein-protein interactionsSpecific protein interactionsDirect interactionClone 9 cellsEndothelial nitric oxide synthaseMembrane compartmentsLarge GTPaseGlutathione S-transferaseProtein interactionsNovel functionDynaminECV-304 cellsSynthase functionIntracellular signalsMembrane distributionConfocal immunofluorescenceFluorescent proteinENOS regulationGreen fluorescentProtein
1999
Molecular control of nitric oxide synthases in the cardiovascular system
Papapetropoulos A, Rudic R, Sessa W. Molecular control of nitric oxide synthases in the cardiovascular system. Cardiovascular Research 1999, 43: 509-520. PMID: 10690323, DOI: 10.1016/s0008-6363(99)00161-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood PressureCardiovascular DiseasesCardiovascular SystemCentral Nervous SystemGene Expression RegulationHomeostasisHumansImmune SystemMiceMice, KnockoutNeovascularization, PathologicNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type INitric Oxide Synthase Type IINitric Oxide Synthase Type IIIRatsVasomotor SystemHsp90 regulation of endothelial nitric oxide synthase contributes to vascular control in portal hypertension
Shah V, Wiest R, Garcia-Cardena G, Cadelina G, Groszmann R, Sessa W. Hsp90 regulation of endothelial nitric oxide synthase contributes to vascular control in portal hypertension. American Journal Of Physiology 1999, 277: g463-g468. PMID: 10444461, DOI: 10.1152/ajpgi.1999.277.2.g463.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsBenzoquinonesBlood VesselsHSP90 Heat-Shock ProteinsHypertension, PortalIn Vitro TechniquesLactams, MacrocyclicMaleMethoxamineMicrocirculationNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIQuinonesRatsRats, Sprague-DawleySplanchnic CirculationTissue DistributionVasoconstrictor AgentsVasodilationVasodilator AgentsConceptsEndothelial nitric oxide synthasePortal vein ligationNitric oxide synthasePortal hypertensionMesenteric vasculatureOxide synthaseNormal animalsACh-dependent vasodilationExperimental portal hypertensionExcessive NO productionNO-dependent responsesNOS catalytic activityDependent vasodilationVein ligationVascular controlMesenteric circulationPVL animalsMesenteric vesselsHeat shock protein 90Sodium nitroprussideNO productionEndothelial liningHypertensionShock protein 90MethoxamineRegulation of endothelium-derived nitric oxide production by the protein kinase Akt
Fulton D, Gratton J, McCabe T, Fontana J, Fujio Y, Walsh K, Franke T, Papapetropoulos A, Sessa W. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 1999, 399: 597-601. PMID: 10376602, PMCID: PMC3637917, DOI: 10.1038/21218.Peer-Reviewed Original ResearchConceptsProtein kinase AktKinase AktSerine/threonine protein kinase AktMutant eNOSRole of phosphorylationEndothelial nitric oxide synthaseSerine 1179Akt substrateSignal transductionGene transferAktAdenovirus-mediated gene transferPhosphorylationGrowth factorVascular endothelial growth factorEndothelial cellsRegulationSynthase isoformsEndothelial growth factorNitric oxide productionTransductionVascular remodellingOxide productionIsoformsProductionNO overproduction by eNOS precedes hyperdynamic splanchnic circulation in portal hypertensive rats
Wiest R, Shah V, Sessa W, Groszmann R. NO overproduction by eNOS precedes hyperdynamic splanchnic circulation in portal hypertensive rats. American Journal Of Physiology 1999, 276: g1043-g1051. PMID: 10198349, DOI: 10.1152/ajpgi.1999.276.4.g1043.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Flow VelocityBlood PressureEndothelium, VascularHypertension, PortalKineticsLuminescent MeasurementsMaleMesenteric Artery, SuperiorMethoxamineNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIINitroarginineRatsRats, Sprague-DawleySplanchnic CirculationStress, MechanicalConceptsHyperdynamic splanchnic circulationSuperior mesenteric arterySplanchnic circulationSham ratsNitric oxide synthase upregulationHyperdynamic circulatory syndromeNO metabolites concentrationPortal hypertensive ratsENOS protein levelsHigh blood flowSignificant hyporesponsivenessArterial vasodilatationL-NNAPortal hypertensionPVL ratsAgonist methoxamineCirculatory syndromeENOS upregulationHypertensive ratsMesenteric arteryNomega-nitroNO inhibitorBlood flowDay 3L-arginineNitric Oxide in Endothelial Dysfunction and Vascular Remodeling: Clinical Correlates and Experimental Links
Rudic R, Sessa W. Nitric Oxide in Endothelial Dysfunction and Vascular Remodeling: Clinical Correlates and Experimental Links. American Journal Of Human Genetics 1999, 64: 673-677. PMID: 10052999, PMCID: PMC1377782, DOI: 10.1086/302304.Peer-Reviewed Original Research
1998
Biological Action of Leptin as an Angiogenic Factor
Sierra-Honigmann M, Nath A, Murakami C, Garcı́a-Cardeña G, Papapetropoulos A, Sessa W, Madge L, Schechner J, Schwabb M, Polverini P, Flores-Riveros J. Biological Action of Leptin as an Angiogenic Factor. Science 1998, 281: 1683-1686. PMID: 9733517, DOI: 10.1126/science.281.5383.1683.Peer-Reviewed Original ResearchMeSH KeywordsAdipocytesAmino Acid SequenceAnimalsCarrier ProteinsCells, CulturedCorneal NeovascularizationDNA-Binding ProteinsEndothelial Growth FactorsEndothelium, VascularEnergy MetabolismHumansLeptinLipid MetabolismLymphokinesMolecular Sequence DataNeovascularization, PhysiologicPhosphorylationProteinsRatsRats, ZuckerReceptors, Cell SurfaceReceptors, LeptinSTAT3 Transcription FactorTrans-ActivatorsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsFa/fa Zucker ratFunctional leptin receptorHuman endothelial cellsNormal ratsFood intakeLeptin receptorVascular endotheliumZucker ratsLeptinOb-RbAngiogenic factorsEndothelial cellsAngiogenic activityPrimary culturesEnergy expenditureBiological actionsHuman vasculatureVivo assaysRatsCorneaReceptorsPhysiological mechanismsNeuronal nitric oxide synthase is expressed in principal cell of collecting duct
Wang X, Lu M, Gao Y, Papapetropoulos A, Sessa W, Wang W. Neuronal nitric oxide synthase is expressed in principal cell of collecting duct. American Journal Of Physiology 1998, 275: f395-f399. PMID: 9729512, DOI: 10.1152/ajprenal.1998.275.3.f395.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseEndothelial nitric oxide synthasePresence of nNOSNitric oxide synthaseOxide synthasePrincipal cellsImmunocytochemical studyPresence of mRNART-PCR techniqueRat neuronal nitric oxide synthaseNegative immunostainingNNOS antibodyRat CCDRat kidneyImmunocytochemical methodsRT-PCRImmunoreactivityRatsDietCellsLight microscopyDuctSynthaseGene-specific primersKidneyHypotension and inflammatory cytokine gene expression triggered by factor Xa–nitric oxide signaling
Papapetropoulos A, Piccardoni P, Cirino G, Bucci M, Sorrentino R, Cicala C, Johnson K, Zachariou V, Sessa W, Altieri D. Hypotension and inflammatory cytokine gene expression triggered by factor Xa–nitric oxide signaling. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 4738-4742. PMID: 9539808, PMCID: PMC22560, DOI: 10.1073/pnas.95.8.4738.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCytokinesEndothelium, VascularFactor XaFibrinogenGene Expression RegulationHistamineHumansHypotensionInflammationInterleukin-6MaleNG-Nitroarginine Methyl EsterNitric OxidePolymerase Chain ReactionRatsRats, WistarSplanchnic CirculationStereoisomerismTumor Necrosis Factor-alphaUmbilical VeinsConceptsEffector cell protease receptor-1Cytokine gene expressionFactor XaReceptor 1NO synthase inhibitor LNitric oxideInflammatory cytokine gene expressionActivation of coagulationNitroarginine methyl esterEndothelial cell releaseTissue factor pathway inhibitorAcute phase responseEndothelial cell ligandsThrombin inhibitor hirudinInterleukin-6 mRNAFactor pathway inhibitorDose-dependent mannerVascular endothelial cellsL-NAMED-NAMEGrowth factor peptidesInterleukin-6Dose-dependent reactionCell ligandsPathway inhibitorDynamic activation of endothelial nitric oxide synthase by Hsp90
García-Cardeña G, Fan R, Shah V, Sorrentino R, Cirino G, Papapetropoulos A, Sessa W. Dynamic activation of endothelial nitric oxide synthase by Hsp90. Nature 1998, 392: 821-824. PMID: 9580552, DOI: 10.1038/33934.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibiotics, AntineoplasticAortaBenzoquinonesCattleCell LineCOS CellsEndothelial Growth FactorsEndothelium, VascularEnzyme ActivationHistamineHSP90 Heat-Shock ProteinsHumansLactams, MacrocyclicLymphokinesMuscle RelaxationNitric OxideNitric Oxide SynthasePrecipitin TestsQuinonesRatsSignal TransductionStress, MechanicalTransfectionVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsActivation of eNOSCellular targetsHeat shock protein 90Binding of HSP90Specific cellular targetsEndothelial nitric oxide synthaseMolecular chaperonesHsp90 associatesSignaling proteinsProtein foldingProtein 90Mechanotransduction pathwaysENOS complexG proteinsFluid shear stressHsp90Activation statePrecise roleGrowth factorDynamic activationVascular endothelial growth factorSynthaseNitric oxide synthaseEndothelial growth factorActivation
1990
Cytochrome P450-Dependent Arachidonic Acid Metabolites, 19− and 20-Hydroxyeicosatetraenoic Acids, Enhance Sodium-Potassium ATPase Activity in Vascular Smooth Muscle
Escalante B, Sessa W, Falck JR, Yadagiri P, Schwartzman M. Cytochrome P450-Dependent Arachidonic Acid Metabolites, 19− and 20-Hydroxyeicosatetraenoic Acids, Enhance Sodium-Potassium ATPase Activity in Vascular Smooth Muscle. Journal Of Cardiovascular Pharmacology 1990, 16: 438-443. PMID: 1700215, DOI: 10.1097/00005344-199009000-00013.Peer-Reviewed Original ResearchConceptsCytochrome P450-dependent arachidonic acid metabolitesPotassium-induced relaxationArachidonic acid metabolitesAcid metabolitesOuabain-sensitive 86Rb uptakeSodium-potassium ATPase activityRat aortic ringsProstaglandin-like materialVascular smooth muscleDose-dependent fashionEffect of omegaAortic ringsSmooth muscleStimulatory effectInhibitory effectATPase activityPresent studyMetabolitesCyclooxygenaseIndomethacinStz-Induced Diabetes in Shr and Renovascular Hypertensive Rats: Dissociation Between Changes in Arterial Pressure and Vascular Collagen Synthesis
Mariani M, Sessa W, Chichester C, Rodgers R. Stz-Induced Diabetes in Shr and Renovascular Hypertensive Rats: Dissociation Between Changes in Arterial Pressure and Vascular Collagen Synthesis. Clinical And Experimental Hypertension 1990, 12: 1003-1019. PMID: 2245511, DOI: 10.3109/10641969009073514.Peer-Reviewed Original ResearchConceptsRenovascular hypertensive ratsVascular collagen synthesisArterial pressureHypertensive ratsCollagen synthesisSTZ induced diabetesLeft renal arteryNormotensive WKY ratsSTZ-induced diabetesWistar-Kyoto ratsNondiabetic SHRRVH ratsRenovascular hypertensionWKY controlsNormotensive controlsRenal arteryWKY ratsProlyl hydroxylase activitySHRDiabetesRatsHydroxylase activityProline incorporationHypertensionWKY
1989
Manipulation of cytochrome P-450 dependent renal thromboxane synthase activity in spontaneously hypertensive rats
Sessa W, Abraham N, Escalante B, Schwartzman M. Manipulation of cytochrome P-450 dependent renal thromboxane synthase activity in spontaneously hypertensive rats. Journal Of Hypertension 1989, 7: 37-42. PMID: 2708811, DOI: 10.1097/00004872-198901000-00006.Peer-Reviewed Original ResearchConceptsHeme oxygenase activityThromboxane synthase activityHypertensive ratsRenal heme oxygenase activitySeven-week-old SHRRenal cortical microsomesSystolic blood pressureMicrosomal heme oxygenase activityRenal cytochrome PCytochrome PSynthase activityThromboxane A2 formationCortical microsomesBlood pressureOxygenase activityBody weightA2 formationHemoprotein synthesisConsecutive daysControl levelsThromboxane synthaseSHRRatsHeme contentHeme availability