1999
Trafficking of Endothelial Nitric-oxide Synthase in Living Cells QUANTITATIVE EVIDENCE SUPPORTING THE ROLE OF PALMITOYLATION AS A KINETIC TRAPPING MECHANISM LIMITING MEMBRANE DIFFUSION*
Sowa G, Liu J, Papapetropoulos A, Rex-Haffner M, Hughes T, Sessa W. Trafficking of Endothelial Nitric-oxide Synthase in Living Cells QUANTITATIVE EVIDENCE SUPPORTING THE ROLE OF PALMITOYLATION AS A KINETIC TRAPPING MECHANISM LIMITING MEMBRANE DIFFUSION*. Journal Of Biological Chemistry 1999, 274: 22524-22531. PMID: 10428829, DOI: 10.1074/jbc.274.32.22524.Peer-Reviewed Original ResearchConceptsPlasma membraneENOS-GFPFluorescent protein fusion constructsProtein-protein interactionsRole of palmitoylationProtein fusion constructsLipid bilayersRate of traffickingEndothelial cell line ECV304Endothelial nitric oxide synthasePalmitoylation stateCellular domainsFusion constructsPerinuclear regionLiving cellsProtein diffusionFluorescence recoveryRegulation of eNOSMembrane markersPalmitoylationMutantsGolgiTraffickingMembrane diffusionEndothelial cells
1997
The First 35 Amino Acids and Fatty Acylation Sites Determine the Molecular Targeting of Endothelial Nitric Oxide Synthase into the Golgi Region of Cells: A Green Fluorescent Protein Study
Liu J, Hughes T, Sessa W. The First 35 Amino Acids and Fatty Acylation Sites Determine the Molecular Targeting of Endothelial Nitric Oxide Synthase into the Golgi Region of Cells: A Green Fluorescent Protein Study. Journal Of Cell Biology 1997, 137: 1525-1535. PMID: 9199168, PMCID: PMC2137822, DOI: 10.1083/jcb.137.7.1525.Peer-Reviewed Original ResearchConceptsPalmitoylation-deficient mutantN-myristoylationNIH 3T3 cellsMembrane associationGolgi regionPalmitoylation sitesAmino acidsGreen fluorescent protein studiesGolgi complexFatty acylation sitesGFP fusion proteinBiochemical studiesFluorescent protein chimerasDiffuse fluorescence patternEndothelial cellsEndothelial nitric oxide synthaseCytosolic natureGFP tagFatty acylationAcylated proteinsGolgi markersProtein chimerasIntracellular membranesAcylation siteIntracellular targeting