1999
NO overproduction by eNOS precedes hyperdynamic splanchnic circulation in portal hypertensive rats
Wiest R, Shah V, Sessa W, Groszmann R. NO overproduction by eNOS precedes hyperdynamic splanchnic circulation in portal hypertensive rats. American Journal Of Physiology 1999, 276: g1043-g1051. PMID: 10198349, DOI: 10.1152/ajpgi.1999.276.4.g1043.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Flow VelocityBlood PressureEndothelium, VascularHypertension, PortalKineticsLuminescent MeasurementsMaleMesenteric Artery, SuperiorMethoxamineNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIINitroarginineRatsRats, Sprague-DawleySplanchnic CirculationStress, MechanicalConceptsHyperdynamic splanchnic circulationSuperior mesenteric arterySplanchnic circulationSham ratsNitric oxide synthase upregulationHyperdynamic circulatory syndromeNO metabolites concentrationPortal hypertensive ratsENOS protein levelsHigh blood flowSignificant hyporesponsivenessArterial vasodilatationL-NNAPortal hypertensionPVL ratsAgonist methoxamineCirculatory syndromeENOS upregulationHypertensive ratsMesenteric arteryNomega-nitroNO inhibitorBlood flowDay 3L-arginineInduction of Nitric Oxide Synthase mRNA by Shear Stress Requires Intracellular Calcium and G-protein Signals and Is Modulated by PI 3 Kinase
Malek A, Jiang L, Lee I, Sessa W, Izumo S, Alper S. Induction of Nitric Oxide Synthase mRNA by Shear Stress Requires Intracellular Calcium and G-protein Signals and Is Modulated by PI 3 Kinase. Biochemical And Biophysical Research Communications 1999, 254: 231-242. PMID: 9920763, DOI: 10.1006/bbrc.1998.9921.Peer-Reviewed Original ResearchConceptsNitric oxide synthase mRNAPTX-sensitive G proteinsENOS mRNA levelsENOS mRNABovine aortic endothelial cellsIntracellular calciumPertussis toxinMRNA upregulationEndothelial nitric oxide synthase (eNOS) mRNAMRNA levelsEndothelin-1 mRNACalmodulin inhibitor WENOS gene promoterG proteinsSynthase mRNAAortic endothelial cellsTime-dependent increaseTyrosine kinase inhibitor herbimycin ACalcium entryBAPTA-AMInhibitor WEndothelial cellsTyrosine kinase activityMicrotubule integrityLaminar fluid shear stress
1998
Dynamic activation of endothelial nitric oxide synthase by Hsp90
García-Cardeña G, Fan R, Shah V, Sorrentino R, Cirino G, Papapetropoulos A, Sessa W. Dynamic activation of endothelial nitric oxide synthase by Hsp90. Nature 1998, 392: 821-824. PMID: 9580552, DOI: 10.1038/33934.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibiotics, AntineoplasticAortaBenzoquinonesCattleCell LineCOS CellsEndothelial Growth FactorsEndothelium, VascularEnzyme ActivationHistamineHSP90 Heat-Shock ProteinsHumansLactams, MacrocyclicLymphokinesMuscle RelaxationNitric OxideNitric Oxide SynthasePrecipitin TestsQuinonesRatsSignal TransductionStress, MechanicalTransfectionVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsActivation of eNOSCellular targetsHeat shock protein 90Binding of HSP90Specific cellular targetsEndothelial nitric oxide synthaseMolecular chaperonesHsp90 associatesSignaling proteinsProtein foldingProtein 90Mechanotransduction pathwaysENOS complexG proteinsFluid shear stressHsp90Activation statePrecise roleGrowth factorDynamic activationVascular endothelial growth factorSynthaseNitric oxide synthaseEndothelial growth factorActivation