2006
P013. Nitric oxide synthase establishes a local nitric oxide gradient regulating S-nitrosylation and protein secretion
Iwakiri Y, Satoh A, Chatterjee S, Shah V, Toomre D, Chalouni C, Fulton D, Groszmann R, Sessa W. P013. Nitric oxide synthase establishes a local nitric oxide gradient regulating S-nitrosylation and protein secretion. Nitric Oxide 2006, 14: 21. DOI: 10.1016/j.niox.2006.04.072.Peer-Reviewed Original ResearchO08. Identification of Golgi localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase
Fernández C, Fukata M, Bernatchez P, Fukata Y, Lin M, Bredt D, Sessa W. O08. Identification of Golgi localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase. Nitric Oxide 2006, 14: 3. DOI: 10.1016/j.niox.2006.04.012.Peer-Reviewed Original Research
2001
Dynamic regulation of metabolism and respiration by endogenously produced nitric oxide protects against oxidative stress
Paxinou E, Weisse M, Chen Q, Souza J, Hertkorn C, Selak M, Daikhin E, Yudkoff M, Sowa G, Sessa W, Ischiropoulos H. Dynamic regulation of metabolism and respiration by endogenously produced nitric oxide protects against oxidative stress. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 11575-11580. PMID: 11562476, PMCID: PMC58771, DOI: 10.1073/pnas.201293198.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseNitric oxideOxidative stressProtective effectNitric oxide protectsNitric oxide synthaseNitric oxide synthesisECV304 cellsSteady-state levelsMechanism of protectionOxide synthaseOxide synthesisENOS cDNAHuman ECV304 cellsMitochondria respirationDeathMitochondrial respirationExposureSame extentCellsCell metabolismDynamic regulationMetabolismLow steady-state levelsGlycolytic pathwayCan modulation of endothelial nitric oxide synthase explain the vasculoprotective actions of statins?
Sessa W. Can modulation of endothelial nitric oxide synthase explain the vasculoprotective actions of statins? Trends In Molecular Medicine 2001, 7: 189-191. PMID: 11325618, DOI: 10.1016/s1471-4914(01)01985-2.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseMainstay of therapyCoronary artery diseaseLipid-lowering effectsNitric oxide synthaseNitric oxide synthesisClass of drugsNitric oxide releaseArtery diseaseVasculoprotective actionsOxide synthaseBeneficial actionsOxide synthesisOxide releaseCellular mechanismsStatinsDrugsRecent insightsTherapyMainstayDiseaseThe phosphorylation of endothelial nitric oxide synthase by Akt increases nitric oxide production in early portal hypertension
Iwakiri Y, Tsai M, Sessa W, Groszmann R. The phosphorylation of endothelial nitric oxide synthase by Akt increases nitric oxide production in early portal hypertension. Gastroenterology 2001, 120: a9. DOI: 10.1016/s0016-5085(08)80044-3.Peer-Reviewed Original ResearchThe phosphorylation of endothelial nitric oxide synthase by Akt increases nitric oxide production in early portal hypertension
IWAKIRI Y, TSAI M, SESSA W, GROSZMANN R. The phosphorylation of endothelial nitric oxide synthase by Akt increases nitric oxide production in early portal hypertension. Gastroenterology 2001, 120: a9-a9. DOI: 10.1016/s0016-5085(01)80044-5.Peer-Reviewed Original Research
2000
Simvastatin upregulates coronary vascular endothelial nitric oxide production in conscious dogs
Mital S, Zhang X, Zhao G, Bernstein R, Smith C, Fulton D, Sessa W, Liao J, Hintze T. Simvastatin upregulates coronary vascular endothelial nitric oxide production in conscious dogs. AJP Heart And Circulatory Physiology 2000, 279: h2649-h2657. PMID: 11087217, DOI: 10.1152/ajpheart.2000.279.6.h2649.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenosineAnimalsAnticholesteremic AgentsConsciousnessCoronary CirculationDogsEndothelium, VascularEnzyme InhibitorsGene Expression Regulation, EnzymologicHeart RateIn Vitro TechniquesMicrocirculationMyocardiumNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIINitritesNitroglycerinOxygen ConsumptionRNA, MessengerSimvastatinVasodilationVasodilator AgentsVeratrineConceptsEndothelial nitric oxide synthaseCoronary blood flowCoronary vasodilationConscious dogsSimvastatin administrationVascular endothelial nitric oxide productionVascular endothelial nitric oxide synthaseNO productionEndothelial nitric oxide productionEndothelium-independent vasodilatorCoronary vascular endotheliumShort-term administrationLipid-lowering effectsNitric oxide synthaseEndothelial NO productionMyocardial oxygen consumptionNitric oxide productionNO-dependent regulationPlasma nitrateGeneral anesthesiaENOS proteinCoronary microvesselsOxide synthaseMongrel dogsENOS mRNAGeldanamycin, an inhibitor of heat shock protein 90 (Hsp90) mediated signal transduction has anti‐inflammatory effects and interacts with glucocorticoid receptor in vivo
Bucci M, Roviezzo F, Cicala C, Sessa W, Cirino G. Geldanamycin, an inhibitor of heat shock protein 90 (Hsp90) mediated signal transduction has anti‐inflammatory effects and interacts with glucocorticoid receptor in vivo. British Journal Of Pharmacology 2000, 131: 13-16. PMID: 10960063, PMCID: PMC1572305, DOI: 10.1038/sj.bjp.0703549.Peer-Reviewed Original ResearchConceptsAnti-inflammatory effectsAnti-inflammatory actionEdema formationRU 486Heat shock protein 90Shock protein 90Endothelial nitric oxide synthaseEndothelium-dependent relaxationAnti-inflammatory dosePotential anti-inflammatory drugsVascular endothelial growth factorNitric oxide synthaseAnti-inflammatory drugsEndothelial growth factorDose-dependent mannerProtein 90Specific inhibitorIntact blood vesselsIntraplantar administrationPaw edemaMiddle arteryOxide synthaseRat aortaTherapeutic rationaleGlucocorticoid receptorEstrogen Stimulates Heat Shock Protein 90 Binding to Endothelial Nitric Oxide Synthase in Human Vascular Endothelial Cells EFFECTS ON CALCIUM SENSITIVITY AND NO RELEASE*
Russell K, Haynes M, Caulin-Glaser T, Rosneck J, Sessa W, Bender J. Estrogen Stimulates Heat Shock Protein 90 Binding to Endothelial Nitric Oxide Synthase in Human Vascular Endothelial Cells EFFECTS ON CALCIUM SENSITIVITY AND NO RELEASE*. Journal Of Biological Chemistry 2000, 275: 5026-5030. PMID: 10671543, DOI: 10.1074/jbc.275.7.5026.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseNitric oxide synthaseHuman umbilical vein endothelial cellsENOS activationOxide synthaseEstrogen receptor antagonist ICINO releaseEndothelium-dependent vasodilationReceptor-mediated modulationReceptor antagonist ICINitric oxide releaseUmbilical vein endothelial cellsVein endothelial cellsAntagonist ICIHeat shock protein 90CGMP productionShock protein 90Oxide releaseEndothelial cellsEndothelial cell effectsCalcium sensitivityCalcium dependenceCell effectsEstrogenProtein 90
1999
Hsp90 regulation of endothelial nitric oxide synthase contributes to vascular control in portal hypertension
Shah V, Wiest R, Garcia-Cardena G, Cadelina G, Groszmann R, Sessa W. Hsp90 regulation of endothelial nitric oxide synthase contributes to vascular control in portal hypertension. American Journal Of Physiology 1999, 277: g463-g468. PMID: 10444461, DOI: 10.1152/ajpgi.1999.277.2.g463.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsBenzoquinonesBlood VesselsHSP90 Heat-Shock ProteinsHypertension, PortalIn Vitro TechniquesLactams, MacrocyclicMaleMethoxamineMicrocirculationNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIQuinonesRatsRats, Sprague-DawleySplanchnic CirculationTissue DistributionVasoconstrictor AgentsVasodilationVasodilator AgentsConceptsEndothelial nitric oxide synthasePortal vein ligationNitric oxide synthasePortal hypertensionMesenteric vasculatureOxide synthaseNormal animalsACh-dependent vasodilationExperimental portal hypertensionExcessive NO productionNO-dependent responsesNOS catalytic activityDependent vasodilationVein ligationVascular controlMesenteric circulationPVL animalsMesenteric vesselsHeat shock protein 90Sodium nitroprussideNO productionEndothelial liningHypertensionShock protein 90MethoxamineA vascular bed–specific pathway regulates cardiac expression of endothelial nitric oxide synthase
Guillot P, Guan J, Liu L, Kuivenhoven J, Rosenberg R, Sessa W, Aird W. A vascular bed–specific pathway regulates cardiac expression of endothelial nitric oxide synthase. Journal Of Clinical Investigation 1999, 103: 799-805. PMID: 10079100, PMCID: PMC408151, DOI: 10.1172/jci6017.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthase geneEndothelial nitric oxide synthaseMurine endothelial progenitor cellsGrowth factor antibodyNitric oxide synthase geneNitric oxide synthaseEndothelial progenitor cellsOxide synthase geneFactor antibodyOxide synthaseVascular bedResponse elementCardiac expressionTransgenic micePDGF-ABCardiac endotheliumEno expressionCardiac myocytesSkeletal muscleProgenitor cellsSkeletal myocytesEndotheliumMiceBrainBeta-galactosidase activity
1998
Neuronal nitric oxide synthase is expressed in principal cell of collecting duct
Wang X, Lu M, Gao Y, Papapetropoulos A, Sessa W, Wang W. Neuronal nitric oxide synthase is expressed in principal cell of collecting duct. American Journal Of Physiology 1998, 275: f395-f399. PMID: 9729512, DOI: 10.1152/ajprenal.1998.275.3.f395.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseEndothelial nitric oxide synthasePresence of nNOSNitric oxide synthaseOxide synthasePrincipal cellsImmunocytochemical studyPresence of mRNART-PCR techniqueRat neuronal nitric oxide synthaseNegative immunostainingNNOS antibodyRat CCDRat kidneyImmunocytochemical methodsRT-PCRImmunoreactivityRatsDietCellsLight microscopyDuctSynthaseGene-specific primersKidney
1997
Regulation of Endothelial Nitric Oxide Synthase by Estrogen
Caulin-Glaser T, Sessa W, Bender J. Regulation of Endothelial Nitric Oxide Synthase by Estrogen. Ernst Schering Foundation Symposium Proceedings 1997, 217-232. DOI: 10.1007/978-3-662-03503-0_9.Peer-Reviewed Original ResearchNitric oxideL-arginineAbnormal endothelial cell functionImportant endothelium-derived vasodilatorsEndothelial nitric oxide synthaseVascular smooth muscle cell proliferationVascular smooth muscle relaxationEndothelium-derived vasodilatorsSmooth muscle cell proliferationInhibits neointima formationNitric oxide synthaseSmooth muscle relaxationDifferent NOS isoformsNitric oxide synthase regulationMuscle cell proliferationSoluble guanylate cyclaseEndothelial cell functionNitric oxide synthase (NOS) familyVascular relaxationPlatelet aggregabilityVascular diseaseEndothelial NOSOxide synthaseAntiatherogenic propertiesMuscle relaxation
1996
Nitric oxide synthase induction with renal transplant rejection or infection
Smith S, Wheeler M, Zhang R, Weiss E, Lorber M, Sessa W, Weiss R. Nitric oxide synthase induction with renal transplant rejection or infection. Kidney International 1996, 50: 2088-2093. PMID: 8943494, DOI: 10.1038/ki.1996.533.Peer-Reviewed Original ResearchConceptsUrinary tract infectionRenal transplant patientsNitric oxide synthaseCyclic GMP levelsTransplant patientsTract infectionsNOS activityPost-renal transplantation patientsGMP levelsInducible nitric oxide synthaseNitric oxideRenal allograft rejectionRenal transplant rejectionNitric oxide synthase inductionInducible NOS (iNOS) mRNAUrine of patientsRenal transplantationTransplantation patientsAllograft rejectionTransplant rejectionInducible NOSOxide synthaseNOS mRNAPatientsSynthase inductionElevated blood pressures in mice lacking endothelial nitric oxide synthase
Shesely E, Maeda N, Kim H, Desai K, Krege J, Laubach V, Sherman P, Sessa W, Smithies O. Elevated blood pressures in mice lacking endothelial nitric oxide synthase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 13176-13181. PMID: 8917564, PMCID: PMC24066, DOI: 10.1073/pnas.93.23.13176.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsBlood PressureCattleChimeraDNA PrimersEndothelium, VascularFemaleGenotypeHeterozygoteHypertensionIsoenzymesKidneyLipopolysaccharidesMaleMiceMice, Inbred C57BLMice, KnockoutNitric Oxide SynthasePolymerase Chain ReactionReninRNA, MessengerStem CellsTranscription, GeneticConceptsEndothelial nitric oxide synthaseBlood pressureNitric oxide synthaseHeart rateENOS proteinOxide synthaseENOS mutant miceLipopolysaccharide-induced deathElevated blood pressureNormal blood pressurePlasma renin concentrationBlood pressure regulationLower body weightKidney renin mRNAAnti-eNOS antibodiesAppropriate genetic controlsENOS locusENOS genotypesRenin concentrationVascular toneFemale miceRenin mRNAENOS geneENOS mutationBody weight
1995
The Golgi Association of Endothelial Nitric Oxide Synthase Is Necessary for the Efficient Synthesis of Nitric Oxide(∗)
Sessa W, Garca-Cardea G, Liu J, Keh A, Pollock J, Bradley J, Thiru S, Braverman I, Desai K. The Golgi Association of Endothelial Nitric Oxide Synthase Is Necessary for the Efficient Synthesis of Nitric Oxide(∗). Journal Of Biological Chemistry 1995, 270: 17641-17644. PMID: 7543089, DOI: 10.1074/jbc.270.30.17641.Peer-Reviewed Original ResearchConceptsHEK-293 cellsHeterologous expression systemEndothelial nitric oxide synthaseNovel GolgiGolgi associationExpression systemIntracellular signalsCultured endothelial cellsGolgiIntact blood vesselsEnzymeParticulate enzymeProteinEndothelial cellsCellsSynthaseNitric oxide synthaseOxide synthaseBlood vesselsCompartmentalizationNitric oxideTarget
1994
The Nitric Oxide Synthase Family of Proteins
Sessa W. The Nitric Oxide Synthase Family of Proteins. Journal Of Vascular Research 1994, 31: 131-143. PMID: 7511942, DOI: 10.1159/000159039.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid OxidoreductasesAmino Acid SequenceAnimalsArginineCardiovascular SystemCloning, MolecularConsensus SequenceDNAGene Expression Regulation, EnzymologicHumansImmune SystemMolecular Sequence DataNervous SystemNitric OxideNitric Oxide SynthasePhosphorylationSequence AlignmentSequence Homology, Amino AcidConceptsCytochrome P450 reductase domainNitric oxide synthaseP450 reductase domainNitric oxide synthase (NOS) familySynthase familyMammalian enzymeSeparate genesReductase domainMolecular biologyNovel familyIsoforms of NOSImportant familyNitric oxideProteinEndothelial nitric oxide synthaseEnzyme nitric oxide synthaseGuanidino nitrogenImportant bio-regulatory moleculeOxide synthaseFamilyL-arginineCardiovascular systemRecent advancesGenesEnzymology