1996
Characterization of Bovine Endothelial Nitric Oxide Synthase Expressed inE. coli
Martasek P, Liu Q, Liu J, Roman L, Gross S, Sessa W, Masters B. Characterization of Bovine Endothelial Nitric Oxide Synthase Expressed inE. coli. Biochemical And Biophysical Research Communications 1996, 219: 359-365. PMID: 8604992, DOI: 10.1006/bbrc.1996.0238.Peer-Reviewed Original ResearchConceptsE. coliBaculovirus expression systemBovine endothelial nitric oxide synthaseSDS/PAGEHEK-293 cellsChaperonin GroELHeterologous expressionRecombinant enzymeExpression systemExpression vectorActive enzymeFormation assaysEnzymatic activityColiEnzymeTissue sourcesEndothelial constitutive nitric oxide synthaseProteinSingle bandSynthaseNitric oxide synthaseAbsorbance maximumEndothelial nitric oxide synthasePalmitoylation of Endothelial Nitric Oxide Synthase Is Necessary for Optimal Stimulated Release of Nitric Oxide: Implications for Caveolae Localization †
Liu J, García-Cardeña G, Sessa W. Palmitoylation of Endothelial Nitric Oxide Synthase Is Necessary for Optimal Stimulated Release of Nitric Oxide: Implications for Caveolae Localization †. Biochemistry 1996, 35: 13277-13281. PMID: 8873592, DOI: 10.1021/bi961720e.Peer-Reviewed Original ResearchConceptsPalmitoylation-deficient mutantMembrane associationN-myristoylationWT enzymeLocalization of eNOSActivation of eNOSEndothelial nitric oxide synthaseCaveolae membranesCaveolar localizationPalmitoylationCysteine palmitoylationWT proteinGolgi complexMutantsIntact cellsCaveolaeVivo significanceHigh saltEnzymeCellsSynthaseNitric oxide synthaseTriton XLocalizationImportant role
1995
The Golgi Association of Endothelial Nitric Oxide Synthase Is Necessary for the Efficient Synthesis of Nitric Oxide(∗)
Sessa W, Garca-Cardea G, Liu J, Keh A, Pollock J, Bradley J, Thiru S, Braverman I, Desai K. The Golgi Association of Endothelial Nitric Oxide Synthase Is Necessary for the Efficient Synthesis of Nitric Oxide(∗). Journal Of Biological Chemistry 1995, 270: 17641-17644. PMID: 7543089, DOI: 10.1074/jbc.270.30.17641.Peer-Reviewed Original ResearchConceptsHEK-293 cellsHeterologous expression systemEndothelial nitric oxide synthaseNovel GolgiGolgi associationExpression systemIntracellular signalsCultured endothelial cellsGolgiIntact blood vesselsEnzymeParticulate enzymeProteinEndothelial cellsCellsSynthaseNitric oxide synthaseOxide synthaseBlood vesselsCompartmentalizationNitric oxideTarget