2004
Investigations of Photolysis and Rebinding Kinetics in Myoglobin Using Proximal Ligand Replacements †
Cao W, Ye X, Sjodin T, Christian J, Demidov A, Berezhna S, Wang W, Barrick D, Sage J, Champion P. Investigations of Photolysis and Rebinding Kinetics in Myoglobin Using Proximal Ligand Replacements †. Biochemistry 2004, 43: 11109-11117. PMID: 15323570, DOI: 10.1021/bi049077g.Peer-Reviewed Original ResearchConceptsCO rebinding kineticsRebinding kineticsDiatomic ligandsRaman spectraH93G myoglobinLigand vibrational modesLaser flash photolysisResonance Raman spectraBind exogenous ligandsWild-type MbCOCO rebinding ratesTime-resolved Raman spectroscopyProximal ligandFlash photolysisGeminate phaseVibrational modesProximal linkageLigandRebinding rateKinetic resultsExogenous ligandsPhotolysisKineticsHemeMbCO
2001
Water Penetration and Binding to Ferric Myoglobin †
Cao W, Christian J, Champion P, Rosca F, Sage J. Water Penetration and Binding to Ferric Myoglobin †. Biochemistry 2001, 40: 5728-5737. PMID: 11341838, DOI: 10.1021/bi010067e.Peer-Reviewed Original ResearchConceptsH2O bindingHeme pocketHydrogen bondsHis-64Heme ironFlash photolysis investigationsPhotodissociation of NOFerric heme proteinsH2O ligandsWater moleculesNO photolysisHorse heart metmyoglobinHeme proteinsCO escapeBound waterRebinding rateSmall moleculesH2OPhotolysisDissociation constantBondsHydrogenHemeMoleculesPhysiological NO concentrations
2000
Femtosecond Multicolor Pump−Probe Spectroscopy of Ferrous Cytochrome c †
Wang W, Ye X, Demidov A, Rosca F, Sjodin T, Cao W, Sheeran M, Champion P. Femtosecond Multicolor Pump−Probe Spectroscopy of Ferrous Cytochrome c †. The Journal Of Physical Chemistry B 2000, 104: 10789-10801. DOI: 10.1021/jp0008602.Peer-Reviewed Original ResearchFemtosecond coherence spectroscopyLigand photolysisQuantum yieldAbsorption spectraTransient absorption spectraFerrous cytochrome cHigh quantum yieldUltrafast laser spectroscopyTi:sapphire laserInduce coherent oscillationsLigand recombinationMethionine ligandGeminate rebindingPhotolysis timeQ-bandCm-1Pulsed photoexcitationVibrational modesStructural relaxationCoherence spectroscopyHeme ironMP-8PhotolysisCoherent oscillationsLaser spectroscopy