2022
The flagellar motor protein FliL forms a scaffold of circumferentially positioned rings required for stator activation
Tachiyama S, Chan KL, Liu X, Hathroubi S, Li W, Peterson B, Khan M, Ottemann K, Liu J, Roujeinikova A. The flagellar motor protein FliL forms a scaffold of circumferentially positioned rings required for stator activation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2118401119. PMID: 35046042, PMCID: PMC8794807, DOI: 10.1073/pnas.2118401119.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacterial Physiological PhenomenaBacterial ProteinsFlagellaHelicobacter pyloriMembrane ProteinsModels, MolecularMolecular Motor ProteinsMultiprotein ComplexesProtein BindingProtein ConformationProtein Interaction Domains and MotifsProtein TransportStructure-Activity RelationshipConceptsStator unitsStomatin/prohibitin/flotillin/HflK/C (SPFH) domainWild-type cellsSignificant structural similarityPeriplasmic domainAssembly factorsFlagellar motorAccessory proteinsFliLLinker regionActive conformationFlagellar baseC-domainMotBStructural similarityStator assemblyProteinPutative mechanismsElectron tomography reconstructionsIntact motorCellsActivationDomainMotAHelix
2020
Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles
Lu M, Uchil PD, Li W, Zheng D, Terry DS, Gorman J, Shi W, Zhang B, Zhou T, Ding S, Gasser R, Prévost J, Beaudoin-Bussières G, Anand SP, Laumaea A, Grover JR, Liu L, Ho DD, Mascola JR, Finzi A, Kwong PD, Blanchard SC, Mothes W. Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles. Cell Host & Microbe 2020, 28: 880-891.e8. PMID: 33242391, PMCID: PMC7664471, DOI: 10.1016/j.chom.2020.11.001.Peer-Reviewed Original ResearchConceptsSingle-molecule fluorescence resonance energy transferReceptor-binding domainSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Respiratory syndrome coronavirus 2Conformational dynamicsSyndrome coronavirus 2SARS-CoV-2 spikeCoronavirus disease 2019Distinct conformational statesMechanism of neutralizationHuman receptor angiotensinVirus particlesConvalescent plasmaCoronavirus 2Disease 2019Enzyme 2Fluorescence resonance energy transferReceptor angiotensinVaccine developmentImmunogen designViral entryConformational changesDistinct conformationsS recognition
2015
ORF33 and ORF38 of Kaposi's Sarcoma-Associated Herpesvirus Interact and Are Required for Optimal Production of Infectious Progeny Viruses
Wu J, Avey D, Li W, Gillen J, Fu B, Miley W, Whitby D, Zhu F. ORF33 and ORF38 of Kaposi's Sarcoma-Associated Herpesvirus Interact and Are Required for Optimal Production of Infectious Progeny Viruses. Journal Of Virology 2015, 90: 1741-1756. PMID: 26637455, PMCID: PMC4734004, DOI: 10.1128/jvi.02738-15.Peer-Reviewed Original ResearchMeSH KeywordsCapsid ProteinsGene DeletionGene Expression ProfilingHerpesvirus 8, HumanHumansProtein BindingVirus ReplicationConceptsProgeny virionsCytoplasmic vesiclesKaposi's Sarcoma-Associated Herpesvirus InteractsDouble null mutantsEarly endosome membraneVirion-containing vesiclesMembranes of vesiclesLytic replicationViral gene expressionProgeny virion productionViral lytic replicationEndosome membraneHerpesvirus assemblyGolgi membranesComponent proteinsIdentical phenotypesGene expressionDelicate regulationVirion proteinsVirus assemblyVirus propagationExact functionInfectious progeny virusMature virionsViral assembly
2014
Activation of p90 Ribosomal S6 Kinases by ORF45 of Kaposi's Sarcoma-Associated Herpesvirus Is Critical for Optimal Production of Infectious Viruses
Fu B, Kuang E, Li W, Avey D, Li X, Turpin Z, Valdes A, Brulois K, Myoung J, Zhu F. Activation of p90 Ribosomal S6 Kinases by ORF45 of Kaposi's Sarcoma-Associated Herpesvirus Is Critical for Optimal Production of Infectious Viruses. Journal Of Virology 2014, 89: 195-207. PMID: 25320298, PMCID: PMC4301102, DOI: 10.1128/jvi.01937-14.Peer-Reviewed Original ResearchConceptsRibosomal S6 kinaseP90 ribosomal S6 kinaseKaposi's sarcoma-associated herpesvirusViral gene expressionKSHV lytic replicationSarcoma-associated herpesvirusRSK activationS6 kinaseGene expressionVirus-infected cellsLytic reactivationWild-type virus-infected cellsMutant virus-infected cellsBacterial artificial chromosomeAlanine-scanning mutagenesisLytic replicationCritical rolePhosphoproteomic profilesArtificial chromosomesKinase ERKScanning mutagenesisProgeny viral particlesKSHV genomeInfectious progeny virionsORF45