2021
RSK1 SUMOylation is required for KSHV lytic replication
Liu Z, Liu C, Wang X, Li W, Zhou J, Dong P, Xiao M, Wang C, Zhang Y, Fu J, Zhu F, Liang Q. RSK1 SUMOylation is required for KSHV lytic replication. PLOS Pathogens 2021, 17: e1010123. PMID: 34871326, PMCID: PMC8675914, DOI: 10.1371/journal.ppat.1010123.Peer-Reviewed Original ResearchConceptsSUMO-interacting motifKSHV lytic replicationKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusEfficient KSHV lytic replicationLytic replicationDownstream substrate phosphorylationAmino acid position 166SUMO-SIM interactionsOverall phosphorylation levelMultiple cellular processesPost-translational modificationsKSHV ORF45Substrate phosphorylationSUMO modificationCellular processesDownstream substratesRSK1 activationDownstream kinasesMAPK pathwaySequence analysisRSK1SUMOylationPhosphorylation levelsPosition 166
2015
Phosphoproteomic Analysis of KSHV-Infected Cells Reveals Roles of ORF45-Activated RSK during Lytic Replication
Avey D, Tepper S, Li W, Turpin Z, Zhu F. Phosphoproteomic Analysis of KSHV-Infected Cells Reveals Roles of ORF45-Activated RSK during Lytic Replication. PLOS Pathogens 2015, 11: e1004993. PMID: 26133373, PMCID: PMC4489790, DOI: 10.1371/journal.ppat.1004993.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCell LineGene Expression Regulation, ViralGene Knockout TechniquesHerpesviridae InfectionsHerpesvirus 8, HumanHost-Parasite InteractionsHumansImmediate-Early ProteinsImmunoprecipitationMass SpectrometryPhosphorylationProteomicsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRibosomal Protein S6 Kinases, 90-kDaTransfectionVirus ReplicationConceptsKaposi's Sarcoma-Associated HerpesvirusKSHV lytic replicationProgeny virion productionKSHV ORF45Putative substratesRSK activityExtracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) pathwayGene expressionEfficient KSHV lytic replicationLytic replicationP90 ribosomal S6 kinaseMitogen-activated protein kinase pathwayRSK-dependent phosphorylationHost cell machineryRibosomal S6 kinaseProtein kinase pathwayVirion productionActivation of RSKComplex secondary structureKSHV lytic gene expressionTranscriptional regulatorsDifferential phosphorylationLytic gene expressionCellular genesCell machinery