2019
Chapter Eight Illuminating the virus life cycle with single-molecule FRET imaging
Lu M, Ma X, Mothes W. Chapter Eight Illuminating the virus life cycle with single-molecule FRET imaging. Advances In Virus Research 2019, 105: 239-273. PMID: 31522706, PMCID: PMC7246055, DOI: 10.1016/bs.aivir.2019.07.004.Peer-Reviewed Original ResearchConceptsVirus life cycleMolecular mechanismsViral proteinsSingle-molecule FRETBiological moleculesPrecise molecular mechanismsFörster resonance energy transfer (FRET) imagingLife cycleConformational dynamicsReal-time imagingEnergetics of transitionsNovel antiviral therapiesStructural intermediatesConformational changesConformational statesNative stateSmFRETRelevant conditionsElegant experimental designsAsymmetric intermediatesIntermediatesViral fusionViral glycoproteinsStructural methodsIntact virions
1997
Molecular Mechanism of Membrane Protein Integration into the Endoplasmic Reticulum
Mothes W, Heinrich S, Graf R, Nilsson I, von Heijne G, Brunner J, Rapoport T. Molecular Mechanism of Membrane Protein Integration into the Endoplasmic Reticulum. Cell 1997, 89: 523-533. PMID: 9160744, DOI: 10.1016/s0092-8674(00)80234-2.Peer-Reviewed Original ResearchConceptsTranslocation channelCytosolic domainTransmembrane sequenceMembrane proteinsMolecular mechanismsEndoplasmic reticulumHydrophobic transmembrane sequenceMembrane protein integrationHydrophilic polypeptide segmentsProtein integrationLipid environmentPolypeptide segmentsProteinReticulumLipid phaseSequenceMembraneRibosomesCytosolFoldingDomainMechanismAssemblySignificant implicationsTranslation