A Common Regulatory Signature Associated with Barrier Insulators in Human Primary Erythroid Cells
Steiner L, Maksimova Y, Schulz V, Gallagher P. A Common Regulatory Signature Associated with Barrier Insulators in Human Primary Erythroid Cells. Blood 2010, 116: 3868. DOI: 10.1182/blood.v116.21.3868.3868.Peer-Reviewed Original ResearchGenome-wide mapsPrimary erythroid cellsErythroid cellsProximal promoterChromatin domainsBarrier insulatorsChIP-seqRegulatory signaturesMammalian cellsGene promoterEnhancer-blocking insulator activityTissue-specific gene expressionUpstream stimulatory factor proteinsHeterochromatin-mediated geneGenome-wide occupancyRole of CTCFRecruitment of enzymesΒ-globin locusHistone methyltransferase activityHuman primary erythroid cellsSpecific transcription factorsChicken β-globin locusProximal promoter regionProtein CTCFChromatin boundariesDynamic CO-Localization of GATA1, NFE2, and EKLF and Changes in Gene Expression During Hematopoiesis
Steiner L, Schulz V, Maksimova Y, Mahajan M, Bodine D, Gallagher P. Dynamic CO-Localization of GATA1, NFE2, and EKLF and Changes in Gene Expression During Hematopoiesis. Blood 2010, 116: 741. DOI: 10.1182/blood.v116.21.741.741.Peer-Reviewed Original ResearchErythroid cellsGene expressionTranscription factorsProximal promoterCell type-specific gene expressionHuman beta-globin gene locusBeta-globin gene locusErythrocyte membrane protein genesErythroid-expressed genesGenome-wide scaleGlobin gene locusDNA-protein interactionsHuman primary erythroid cellsNF-E2Primary erythroid cellsMembrane protein geneSpecific gene expressionCritical regulatory elementsPrimary hematopoietic stemCommon regulatory siteUltrahigh-throughput sequencingLineage choiceChromatin immunoprecipitationCoordinated bindingEKLF